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International Journal of Molecular Medicine
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Article

Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway

  • Authors:
    • Lingling Tang
    • Qingge Chen
    • Li Sun
    • Linyun Zhu
    • Jinjin Liu
    • Ziyu Meng
    • Zhenhua Ni
    • Xiongbiao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China, Central Laboratory, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China
  • Pages: 497-504
    |
    Published online on: April 2, 2018
       https://doi.org/10.3892/ijmm.2018.3609
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Abstract

Excessive mucin production in the airway may contribute to airway inflammatory diseases. Curcumin has been reported to prevent mucin 5AC (MUC5AC) production in human airway epithelial cells; however, the molecular targets of curcumin involved in regulating MUC5AC expression have remained elusive. The present study aimed to elucidate the molecular mechanisms by which curcumin regulates MUC5AC production, utilizing the NCI‑H292 human airway epithelial cell line featuring MUC5AC hypersecretion. Curcumin was able to counteract the endothelial growth factor (EGF)‑stimulated mRNA and protein expression of MUC5AC. In addition, curcumin treatment prevented EGF‑induced phosphorylation of EGF receptor (EGFR) as well as the downstream AKT and signal transducer and activator of transcription 3 (STAT3), while inhibition of PI3K and STAT3 signaling significantly attenuated the expression of MUC5AC that was induced by EGF. Furthermore, EGF‑induced increases in the levels of phosphorylated STAT3 in the nuclear fraction were inhibited by curcumin and PI3K inhibitors. In addition, treatment with curcumin significantly decreased MUC5AC and EGFR expression in a time‑dependent manner under basal conditions. These results demonstrated that curcumin inhibited MUC5AC protein expression in NCI‑H292 cells under basal conditions as well under EGF stimulation. This inhibition was accompanied by decreased activation of the EGFR/AKT/STAT3 pathway and reduced EGFR expression, which indicated that curcumin may have a dual role in interfering with the EGFR signaling pathway and inhibiting mucin expression in human airway epithelial cells.
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Copy and paste a formatted citation
Spandidos Publications style
Tang L, Chen Q, Sun L, Zhu L, Liu J, Meng Z, Ni Z and Wang X: Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway. Int J Mol Med 42: 497-504, 2018.
APA
Tang, L., Chen, Q., Sun, L., Zhu, L., Liu, J., Meng, Z. ... Wang, X. (2018). Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway. International Journal of Molecular Medicine, 42, 497-504. https://doi.org/10.3892/ijmm.2018.3609
MLA
Tang, L., Chen, Q., Sun, L., Zhu, L., Liu, J., Meng, Z., Ni, Z., Wang, X."Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway". International Journal of Molecular Medicine 42.1 (2018): 497-504.
Chicago
Tang, L., Chen, Q., Sun, L., Zhu, L., Liu, J., Meng, Z., Ni, Z., Wang, X."Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway". International Journal of Molecular Medicine 42, no. 1 (2018): 497-504. https://doi.org/10.3892/ijmm.2018.3609
Copy and paste a formatted citation
x
Spandidos Publications style
Tang L, Chen Q, Sun L, Zhu L, Liu J, Meng Z, Ni Z and Wang X: Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway. Int J Mol Med 42: 497-504, 2018.
APA
Tang, L., Chen, Q., Sun, L., Zhu, L., Liu, J., Meng, Z. ... Wang, X. (2018). Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway. International Journal of Molecular Medicine, 42, 497-504. https://doi.org/10.3892/ijmm.2018.3609
MLA
Tang, L., Chen, Q., Sun, L., Zhu, L., Liu, J., Meng, Z., Ni, Z., Wang, X."Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway". International Journal of Molecular Medicine 42.1 (2018): 497-504.
Chicago
Tang, L., Chen, Q., Sun, L., Zhu, L., Liu, J., Meng, Z., Ni, Z., Wang, X."Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway". International Journal of Molecular Medicine 42, no. 1 (2018): 497-504. https://doi.org/10.3892/ijmm.2018.3609
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