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International Journal of Molecular Medicine
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July-2018 Volume 42 Issue 1

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Article

Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos

  • Authors:
    • Caiyun Ma
    • Tengfei Lu
    • Hebao Wen
    • Yanjie Zheng
    • Xiao Han
    • Xongda Ji
    • Weijun Guan
  • View Affiliations / Copyright

    Affiliations: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China, Mudanjiang Normal University, Mudanjiang, Heilongjiang 157011, P.R. China
  • Pages: 525-533
    |
    Published online on: April 17, 2018
       https://doi.org/10.3892/ijmm.2018.3629
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Abstract

Cartilage stem/progenitor cells (CSPCs) are a novel stem cell population and function as promising therapeutic candidates for cell‑based cartilage repair. Until now, numerous existing research materials have been obtained from humans, horses, cows and other mammals, but rarely from sheep. In the present study, CSPCs with potential applications in repairing tissue damage and cell‑based therapy were isolated from 45‑day‑old Small‑tailed Han Sheep embryos, and examined at the cellular and molecular level. The expression level of characteristic surface markers of the fetal sheep CSPCs were also evaluated by immunofluorescence, reverse transcription‑polymerase chain reaction analysis and flow cytometric assays. Biological growth curves were drawn in accordance with cell numbers. Additionally, karyotype analysis showed no marked differences in the in vitro cultured CSPCs and they were genetically stable among different passages. The CSPCs were also capable of adipogenic, osteogenic and chondrogenic lineage progression under the appropriate induction medium in vitro. Together, these findings provide a theoretical basis and experimental evidence for cellular transplant therapy in tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Ma C, Lu T, Wen H, Zheng Y, Han X, Ji X and Guan W: Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos. Int J Mol Med 42: 525-533, 2018.
APA
Ma, C., Lu, T., Wen, H., Zheng, Y., Han, X., Ji, X., & Guan, W. (2018). Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos. International Journal of Molecular Medicine, 42, 525-533. https://doi.org/10.3892/ijmm.2018.3629
MLA
Ma, C., Lu, T., Wen, H., Zheng, Y., Han, X., Ji, X., Guan, W."Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos". International Journal of Molecular Medicine 42.1 (2018): 525-533.
Chicago
Ma, C., Lu, T., Wen, H., Zheng, Y., Han, X., Ji, X., Guan, W."Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos". International Journal of Molecular Medicine 42, no. 1 (2018): 525-533. https://doi.org/10.3892/ijmm.2018.3629
Copy and paste a formatted citation
x
Spandidos Publications style
Ma C, Lu T, Wen H, Zheng Y, Han X, Ji X and Guan W: Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos. Int J Mol Med 42: 525-533, 2018.
APA
Ma, C., Lu, T., Wen, H., Zheng, Y., Han, X., Ji, X., & Guan, W. (2018). Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos. International Journal of Molecular Medicine, 42, 525-533. https://doi.org/10.3892/ijmm.2018.3629
MLA
Ma, C., Lu, T., Wen, H., Zheng, Y., Han, X., Ji, X., Guan, W."Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos". International Journal of Molecular Medicine 42.1 (2018): 525-533.
Chicago
Ma, C., Lu, T., Wen, H., Zheng, Y., Han, X., Ji, X., Guan, W."Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos". International Journal of Molecular Medicine 42, no. 1 (2018): 525-533. https://doi.org/10.3892/ijmm.2018.3629
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