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International Journal of Molecular Medicine
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Article

Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins

  • Authors:
    • Yanli Zeng
    • Zixi Li
    • Jia Shang
    • Yi Kang
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, Zhengzhou University People's Hospital, Henan University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Tongji Medical College of Huazhong University of Science and Technology Affiliated Hospital of Traditional Chinese and Western Medicine, Wuhan, Hubei 430030, P.R. China
  • Pages: 1181-1189
    |
    Published online on: May 16, 2018
       https://doi.org/10.3892/ijmm.2018.3681
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Abstract

Hepatitis B virus (HBV) infection and related liver complications remain severe public health problems worldwide. Previous investigations have shown that small interfering (si)RNAs can offer an effective strategy for the treatment of chronic hepatitis B. The present study aimed to develop a novel siRNA‑delivering system of therapeutic HBV nuclear localization sequence (NLS) siRNAs using the recombinant preS1‑truncated protamine (tP) proteins. The preS1 region of the LHB was used in place of scFv to construct the recombinant preS1‑tP proteins, which were applied to deliver siRNAs targeting the HBV NLS to inhibit HBV replication and infection in HepG2.2.15 cells overexpressing sodium taurocholate cotransporting polypeptide (NTCP). The results revealed that HepG2.2.15 cells with stable NTCP expression (HepG2.2.15‑NTCP cells) transfected with the recombinant lentivirus showed increased expression of NTCP genes. The HBV NLS siRNAs significantly suppressed HBV mRNA content and levels of HBsAg and HBeAg in the HepG2.2.15‑NTCP cells. Recombinant preS1‑tP proteins tagged with His and glutathione S‑transferase were found to enter into HepG2.2.15‑NTCP cells and bind with DNA. The HBV NLS siRNAs were delivered into HepG2.2.15‑NTCP cells by recombinant preS1‑tP proteins, which resulted in decreased expression of HBV mRNA, HBsAg and HBeAg, HBV DNA and covalently closed circular DNA in the HepG2.2.15‑NTCP cells. Therefore, the recombinant preS1‑tP proteins successfully delivered NLS siRNAs into HepG2.2.15 cells and repressed HBV infection and replication.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng Y, Li Z, Shang J and Kang Y: Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins. Int J Mol Med 42: 1181-1189, 2018.
APA
Zeng, Y., Li, Z., Shang, J., & Kang, Y. (2018). Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins. International Journal of Molecular Medicine, 42, 1181-1189. https://doi.org/10.3892/ijmm.2018.3681
MLA
Zeng, Y., Li, Z., Shang, J., Kang, Y."Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins". International Journal of Molecular Medicine 42.2 (2018): 1181-1189.
Chicago
Zeng, Y., Li, Z., Shang, J., Kang, Y."Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins". International Journal of Molecular Medicine 42, no. 2 (2018): 1181-1189. https://doi.org/10.3892/ijmm.2018.3681
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng Y, Li Z, Shang J and Kang Y: Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins. Int J Mol Med 42: 1181-1189, 2018.
APA
Zeng, Y., Li, Z., Shang, J., & Kang, Y. (2018). Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins. International Journal of Molecular Medicine, 42, 1181-1189. https://doi.org/10.3892/ijmm.2018.3681
MLA
Zeng, Y., Li, Z., Shang, J., Kang, Y."Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins". International Journal of Molecular Medicine 42.2 (2018): 1181-1189.
Chicago
Zeng, Y., Li, Z., Shang, J., Kang, Y."Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins". International Journal of Molecular Medicine 42, no. 2 (2018): 1181-1189. https://doi.org/10.3892/ijmm.2018.3681
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