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Article

Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation

  • Authors:
    • Qipeng Zhao
    • Ailing Chen
    • Xiaobo Wang
    • Zhuanzhuan Zhang
    • Yunsheng Zhao
    • Yu Huang
    • Shuanglai Ren
    • Yafei Zhu
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Department of Pharmacology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Ningxia Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China
  • Pages: 1190-1198
    |
    Published online on: May 17, 2018
       https://doi.org/10.3892/ijmm.2018.3691
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Abstract

The present study aimed to investigate the protective effects of dehydrocostuslactone (DHL) against rat hippocampal slice injury caused by oxygen‑glucose deprivation/reoxygenation (OGD/R). Rat hippocampal slice injury was induced by OGD/R in vitro, and the degree of injury was evaluated through a lactate dehydrogenase (LDH) assay and 2,3,5‑triphenyltetrazolium chloride (TTC) staining. The protein expression levels of B‑cell lymphoma-2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), cytochrome c (cyt‑c), apoptotic protease activating factor 1 (apaf‑1), caspase‑9, caspase‑7, caspase‑3, sequestosome 1 (SQSTM1) and microtubule‑associated protein 1 light chain 3 (LC3) were analyzed through western blot analysis. The results showed that 1, 5 and 10 µM DHL decreased the levels of LDH (P<0.05) and increased the A490 value of TTC (P<0.05). Furthermore, the expression of Bcl‑2 was enhanced, and the protein expression levels of Bax, cyt‑c, apaf‑1, caspase‑9, caspase‑7, caspase‑3, SQSTM1 and LC3 were significantly inhibited (P<0.05), compared with those in the OGD/R group. These results suggested that DHL elicited protective effects against hippocampal OGD/R injury, and its underlying mechanism may be associated with inhibiting apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Q, Chen A, Wang X, Zhang Z, Zhao Y, Huang Y, Ren S and Zhu Y: Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation. Int J Mol Med 42: 1190-1198, 2018.
APA
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y. ... Zhu, Y. (2018). Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation. International Journal of Molecular Medicine, 42, 1190-1198. https://doi.org/10.3892/ijmm.2018.3691
MLA
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y., Ren, S., Zhu, Y."Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation". International Journal of Molecular Medicine 42.2 (2018): 1190-1198.
Chicago
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y., Ren, S., Zhu, Y."Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation". International Journal of Molecular Medicine 42, no. 2 (2018): 1190-1198. https://doi.org/10.3892/ijmm.2018.3691
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Q, Chen A, Wang X, Zhang Z, Zhao Y, Huang Y, Ren S and Zhu Y: Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation. Int J Mol Med 42: 1190-1198, 2018.
APA
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y. ... Zhu, Y. (2018). Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation. International Journal of Molecular Medicine, 42, 1190-1198. https://doi.org/10.3892/ijmm.2018.3691
MLA
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y., Ren, S., Zhu, Y."Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation". International Journal of Molecular Medicine 42.2 (2018): 1190-1198.
Chicago
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y., Ren, S., Zhu, Y."Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation". International Journal of Molecular Medicine 42, no. 2 (2018): 1190-1198. https://doi.org/10.3892/ijmm.2018.3691
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