Open Access

miR‑133b regulates proliferation and apoptosis in high‑glucose‑induced human retinal endothelial cells by targeting ras homolog family member A

  • Authors:
    • Jun Yao
    • Jihong Wang
    • Yong Yao
    • Kelei Wang
    • Qianqian Zhou
    • Ying Tang
  • View Affiliations

  • Published online on: May 18, 2018     https://doi.org/10.3892/ijmm.2018.3694
  • Pages: 839-850
  • Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the role of microRNA (miR)‑133b in high‑glucose‑induced human retinal endothelial cells (hRECs), particularly regarding its potential targeting of ras homolog family member A (RhoA). To establish the high‑glucose‑induced diabetic retinopathy (DR) model, hRECs were cultured in high‑glucose medium for 1, 2 and 3 days. An Annexin allophycocyanin (APC)/7‑aminoactinomycin D (7‑AAD) staining assay was performed to measure the apoptosis of hRECs. Next, the cells were transfected with miR‑133b inhibitors or mimics, and the cell proliferation and apoptosis were measured by MTT and Annexin‑APC/7‑AAD staining assays, respectively. In addition, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blotting and immunocytochemistry were used to detect the expression levels of RhoA, Rho‑associated protein kinase 1 (ROCK1), LIM domain kinase 1 (LIMK), myosin light chain (MLC) and phosphorylated (p)‑MLC. It was observed that high‑glucose or miR‑133b inhibitor treatment attenuated the apoptosis of hRECs, and upregulated the mRNA and protein expression levels of RhoA, ROCK1 and LIMK, as well as the p‑MLC protein level, in the hRECs. However, miR‑133b overexpression inhibited the cell proliferation, promoted apoptosis, and downregulated the mRNA and protein levels of RhoA, ROCK1 and LIMK, as well as p‑MLC protein, in high‑glucose‑induced hRECs. In conclusion, overexpression of miR‑133b inhibited the proliferation and promoted apoptosis in a DR cell model by downregulating RhoA expression.
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August-2018
Volume 42 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yao J, Wang J, Yao Y, Wang K, Zhou Q and Tang Y: miR‑133b regulates proliferation and apoptosis in high‑glucose‑induced human retinal endothelial cells by targeting ras homolog family member A. Int J Mol Med 42: 839-850, 2018
APA
Yao, J., Wang, J., Yao, Y., Wang, K., Zhou, Q., & Tang, Y. (2018). miR‑133b regulates proliferation and apoptosis in high‑glucose‑induced human retinal endothelial cells by targeting ras homolog family member A. International Journal of Molecular Medicine, 42, 839-850. https://doi.org/10.3892/ijmm.2018.3694
MLA
Yao, J., Wang, J., Yao, Y., Wang, K., Zhou, Q., Tang, Y."miR‑133b regulates proliferation and apoptosis in high‑glucose‑induced human retinal endothelial cells by targeting ras homolog family member A". International Journal of Molecular Medicine 42.2 (2018): 839-850.
Chicago
Yao, J., Wang, J., Yao, Y., Wang, K., Zhou, Q., Tang, Y."miR‑133b regulates proliferation and apoptosis in high‑glucose‑induced human retinal endothelial cells by targeting ras homolog family member A". International Journal of Molecular Medicine 42, no. 2 (2018): 839-850. https://doi.org/10.3892/ijmm.2018.3694