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Article

Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma

  • Authors:
    • Jianping Zhang
    • Genjian Zheng
    • Lan Zhou
    • Pengcheng Li
    • Man Yun
    • Qi Shi
    • Tingli Wang
    • Xiaotong Wu
  • View Affiliations / Copyright

    Affiliations: School of Clinical Medicine, Hainan Medical University, Haikou, Hainan 571199, P.R. China, Department of Stomatology, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, P.R. China, School of Clinical Medicine, Hainan Medical University, Haikou, Hainan 571199, P.R. China
  • Pages: 2276-2284
    |
    Published online on: July 12, 2018
       https://doi.org/10.3892/ijmm.2018.3769
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Abstract

The activation of Notch signalling induces epithelial‑mesenchymal transition (EMT), but this signalling pathway and its association with EMT in the context of cell motility in oral squamous cell carcinoma (OSCC) remains unclear. The present study aimed to investigate the role of the Notch signalling pathway and EMT in the metastatic potential of OSCC using 2 cell lines, Tca8113 and CAL27. The data demonstrated that zinc finger domain SNAI1 (Snail) knockdown by small interfering RNA decreased the expression of vimentin and increased the expression of epithelial cadherin (E‑cadherin). In addition, silencing Snail also significantly inhibited cell migration in the 2 OSCC cell lines. It was also identified that blocking Notch signalling with the g‑secretase inhibitor DAPT decreased the expression of the EMT markers Snail and vimentin and increased E‑cadherin expression, accompanied by a significant inhibition of cell migration in the 2 OSCC cell lines. These data clearly indicate that Notch signalling mediates EMT to promote metastasis in OSCC cells. Therefore, targeting Notch signalling and its association with EMT may provide novel insights into the mechanism of invasion and metastasis in OSCC and potential therapeutic interventions.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Zheng G, Zhou L, Li P, Yun M, Shi Q, Wang T and Wu X: Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma. Int J Mol Med 42: 2276-2284, 2018.
APA
Zhang, J., Zheng, G., Zhou, L., Li, P., Yun, M., Shi, Q. ... Wu, X. (2018). Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma. International Journal of Molecular Medicine, 42, 2276-2284. https://doi.org/10.3892/ijmm.2018.3769
MLA
Zhang, J., Zheng, G., Zhou, L., Li, P., Yun, M., Shi, Q., Wang, T., Wu, X."Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma". International Journal of Molecular Medicine 42.4 (2018): 2276-2284.
Chicago
Zhang, J., Zheng, G., Zhou, L., Li, P., Yun, M., Shi, Q., Wang, T., Wu, X."Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma". International Journal of Molecular Medicine 42, no. 4 (2018): 2276-2284. https://doi.org/10.3892/ijmm.2018.3769
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Zheng G, Zhou L, Li P, Yun M, Shi Q, Wang T and Wu X: Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma. Int J Mol Med 42: 2276-2284, 2018.
APA
Zhang, J., Zheng, G., Zhou, L., Li, P., Yun, M., Shi, Q. ... Wu, X. (2018). Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma. International Journal of Molecular Medicine, 42, 2276-2284. https://doi.org/10.3892/ijmm.2018.3769
MLA
Zhang, J., Zheng, G., Zhou, L., Li, P., Yun, M., Shi, Q., Wang, T., Wu, X."Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma". International Journal of Molecular Medicine 42.4 (2018): 2276-2284.
Chicago
Zhang, J., Zheng, G., Zhou, L., Li, P., Yun, M., Shi, Q., Wang, T., Wu, X."Notch signalling induces epithelial‑mesenchymal transition to promote metastasis in oral squamous cell carcinoma". International Journal of Molecular Medicine 42, no. 4 (2018): 2276-2284. https://doi.org/10.3892/ijmm.2018.3769
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