Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
October-2018 Volume 42 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2018 Volume 42 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts

  • Authors:
    • Jungik Park
    • Grace Choi
    • Mi‑Jin Yim
    • Jeong Min Lee
    • Jong Su Yoo
    • Won Sun Park
    • Seong Kook Park
    • Saegwang Park
    • Su‑Kil Seo
    • Tae‑Geum Kim
    • Dae‑Sung Lee
    • Il‑Whan Choi
  • View Affiliations / Copyright

    Affiliations: Division of Endocrinology and Metabolism, Department of Internal Medicine, Dong‑Eui Medical Center, Dong‑Eui University, Busan 47227, Republic of Korea, Department of Applied Research, National Marine Biodiversity Institute of Korea, Seocheon, Chungcheongnam‑do 33662, Republic of Korea, Department of Physiology, Kangwon National University School of Medicine, Chuncheon, Gangwon‑do 24341, Republic of Korea , Department of Otorhinolaryngology Head and Neck Surgery, Busan Paik Hospital, Inje University, Busan 47392, Republic of Korea , Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 47392, Republic of Korea , Center for Jeongup Industry‑Academy‑Institute Cooperation, Chonbuk National University, Jeonju, Jeollabuk‑do 54896, Republic of Korea
  • Pages: 2213-2220
    |
    Published online on: July 12, 2018
       https://doi.org/10.3892/ijmm.2018.3770
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Phlorotannins (PTNs), a group of phenolic compounds from seaweeds, have diverse bioactivities. However, there has been no report on their antifibrotic effects during nasal polyp (NP) formation. In the present study, the effect of PTNs on transforming growth factor (TGF)‑β1‑induced profibrotic responses in nasal polyp‑derived fibroblasts (NPDFs) were determined and the relevant signaling pathways were investigated. The expression levels of collagen type‑1 (Col‑1) and fibronectin in NP tissues were measured by western blot analysis and immunohistochemistry. The NPDFs were treated with TGF‑β1 (1 ng/ml) in the presence or absence of PTNs (5‑30 µg/ml). The expression levels of α‑smooth muscle actin (α‑SMA), Col‑1, fibronectin, and phosphorylated‑small mothers against decapentaplegic (Smad)2/3 in NPDFs were measured by western blot analysis. The contractile activity of the NPDFs was determined by a collagen gel contraction assay. Col‑1 and fibronectin proteins were found to be expressed in NP tissues. PTNs had no significant cytotoxic effect on TGF‑β1‑induced NPDFs. TGF‑β1 induced the expression α‑SMA, Col‑1 and fibronectin, and stimulated fibroblast‑mediated contraction of collagen gel. However, pre‑treatment with PTNs inhibited the expression of these proteins. The inhibitory effects were mediated through the suppression of Smad2/3 signaling pathways in TGF‑β1‑induced NPDFs. These resulted suggested that PTNs may be important in inhibiting myofibroblast differentiation and extracellular matrix protein accumulation in NP formation through the Smad2/3 signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Cho JS, Kang JH, Shin JM, Park IH and Lee HM: Inhibitory effect of delphinidin on extracellular matrix production via the MAPK/NF-κB pathway in nasal polyp-derived fibroblasts. Allergy Asthma Immunol Res. 7:276–282. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Pawankar R: Nasal polyposis: An update: Editorial review. Curr Opin Allergy Clin Immunol. 3:1–6. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Jung JW, Park IH, Cho JS and Lee HM: Naringenin inhibits extracellular matrix production via extracellular signal-regulated kinase pathways in nasal polyp-derived fibroblasts. Phytother Res. 27:463–467. 2013. View Article : Google Scholar

4 

Cho JS, Moon YM, Um JY, Moon JH, Park IH and Lee HM: Inhibitory effect of ginsenoside Rg1 on extracellular matrix production via extracellular signal-regulated protein kinase/activator protein 1 pathway in nasal polyp-derived fibroblasts. Exp Biol Med (Maywood). 237:663–669. 2012. View Article : Google Scholar

5 

Kondo S, Kagami S, Urushihara M, Kitamura A, Shimizu M, Strutz F, Müller GA and Kuroda Y: Transforming growth factor-beta1 stimulates collagen matrix remodeling through increased adhesive and contractive potential by human renal fibroblasts. Biochim Biophys Acta. 1693:91–100. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Nakagawa T, Yamane H, Nakai Y, Shigeta T, Takashima T and Takeda Z: Comparative assessment of cell proliferation and accumulation of extracellular matrix in nasal polyps. Acta Otolaryngol Suppl. 538:205–208. 1998. View Article : Google Scholar

7 

Kendall RT and Feghali-Bostwick CA: Fibroblasts in fibrosis: Novel roles and mediators. Front Pharmacol. 5:1232014. View Article : Google Scholar : PubMed/NCBI

8 

Suleria HA, Osborne S, Masci P and Gobe G: Marine-based nutraceuticals: An innovative trend in the food and supplement industries. Mar Drugs. 13:6336–6351. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Lee JC, Hou MF, Huang HW, Chang FR, Yeh CC, Tang JY and Chang HW: Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell Int. 13:552013. View Article : Google Scholar : PubMed/NCBI

10 

Wijesinghe WA and Jeon YJ: Exploiting biological activities of brown seaweed Ecklonia cava for potential industrial applications: A review. Int J Food Sci Nutr. 63:225–235. 2012. View Article : Google Scholar

11 

Sekar D and Kolanjinathan K: Antibacterial activity of marine macroalgae Padina gymnospora and Turbinaria conoides collected from Mandapam Coast of Tamilnadu, India. Int J Adv Res Biol Sci. 2:146–152. 2015.

12 

Kolanjinathan K, Ganesh P and Saranraj P: Pharmacological importance of seaweeds: A Review. World J Fish and Marine Sci. 6:1–15. 2014.

13 

Wijesekara I and Kim SK: Angiotensin-I-converting enzyme (ACE) inhibitors from marine resources: Prospects in the pharmaceutical industry. Mar Drugs. 8:1080–1093. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Eom SH, Kim YM and Kim SK: Antimicrobial effect of phlorotannins from marine brown algae. Food Chem Toxicol. 50:3251–3255. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Vo TS, Ngo DH and Kim SK: Marine algae as a potential pharmaceutical source for anti-allergic therapeutics. Process Biochem. 47:386–394. 2012. View Article : Google Scholar

16 

Lee DS, Lee CM, Park SK, Yim MJ, Lee JM, Choi G, Yoo JS, Jung WK, Park S, Seo SK, et al: Anti-inhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts. Int J Mol Med. 40:1950–1956. 2017.PubMed/NCBI

17 

Jung HA, Jin SE, Ahn BR, Lee CM and Choi JS: Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW264.7 macrophages. Food Chem Toxicol. 59:199–206. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Yang YI, Woo JH, Seo YJ, Lee KT, Lim Y and Choi JH: Protective effect of brown alga phlorotannins against hyper-inflammatory responses in lipopolysaccharide-induced sepsis models. J Agric Food Chem. 64:570–578. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Massagué J, Blain SW and Lo RS: TGFbeta signaling in growth control, cancer, and heritable disorders. Cell. 103:295–309. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Li S, Gu X and Yi S: The regulatory effects of transforming growth factor-β on nerve regeneration. Cell Transplant. 26:381–394. 2017. View Article : Google Scholar :

21 

Pohlers D, Brenmoehl J, Löffler I, Müller CK, Leipner C, Schultze-Mosgau S, Stallmach A, Kinne RW and Wolf G: TGF-beta and fibrosis in different organs - molecular pathway imprints. Biochim Biophys Acta. 1792:746–756. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Chang CH, Chai CY, Ho KY, Kuo WR, Tai CF, Lin CS, Tsai SM, Wu SC and Juan KH: Expression of transforming growth factor-beta 1 and alpha-smooth muscle actin of myofibroblast in the pathogenesis of nasal polyps. Kaohsiung J Med Sci. 17:133–138. 2001.PubMed/NCBI

23 

Coste A, Lefaucheur JP, Wang QP, Lesprit E, Poron F, Peynegre R and Escudier E: Expression of the transforming growth factor beta isoforms in inflammatory cells of nasal polyps. Arch Otolaryngol Head Neck Surg. 124:1361–1366. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Shin JM, Park JH, Park IH and Lee HM: Pirfenidone inhibits transforming growth factor β1-induced extracellular matrix production in nasal polyp-derived fibroblasts. Am J Rhinol Allergy. 29:408–413. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Shin JM, Park JH, Park IH and Lee HM: Doxycycline inhibits TGF-β1-induced extracellular matrix production in nasal polyp-derived fibroblasts. Int Forum Allergy Rhinol. 6:256–263. 2016. View Article : Google Scholar

26 

Park SK, Jin YD, Park YK, Yeon SH, Xu J, Han RN, Rha KS and Kim YM: IL-25-induced activation of nasal fibroblast and its association with the remodeling of chronic rhinosinusitis with nasal polyposis. PLoS One. 12:e01818062017. View Article : Google Scholar : PubMed/NCBI

27 

Sugiura H, Ichikawa T, Liu X, Kobayashi T, Wang XQ, Kawasaki S, Togo S, Kamio K, Mao L, Ann Y, et al: N-acetyl-L-cysteine inhibits TGF-β1-induced profibrotic responses in fibroblasts. Pulm Pharmacol Ther. 22:487–491. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Molet SM, Hamid QA and Hamilos DL: IL-11 and IL-17 expression in nasal polyps: Relationship to collagen deposition and suppression by intranasal fluticasone propionate. Laryngoscope. 113:1803–1812. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Ahn JY, Kim MH, Lim MJ, Park S, Lee SL, Yun YS and Song JY: The inhibitory effect of ginsan on TGF-β mediated fibrotic process. J Cell Physiol. 226:1241–1247. 2011. View Article : Google Scholar

30 

Zhou L, Dong X, Wang L, Shan L, Li T, Xu W, Ding Y, Lai M, Lin X, Dai M, et al: Casticin attenuates liver fibrosis and hepatic stellate cell activation by blocking TGF-β/Smad signaling pathway. Oncotarget. 8:56267–56280. 2017.PubMed/NCBI

31 

Wipff PJ, Rifkin DB, Meister JJ and Hinz B: Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix. J Cell Biol. 179:1311–1323. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Park J, Choi G, Yim MJ, Lee JM, Yoo JS, Park WS, Park SK, Park S, Seo SK, Kim G, Kim G, et al: Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts. Int J Mol Med 42: 2213-2220, 2018.
APA
Park, J., Choi, G., Yim, M., Lee, J.M., Yoo, J.S., Park, W.S. ... Choi, I. (2018). Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts. International Journal of Molecular Medicine, 42, 2213-2220. https://doi.org/10.3892/ijmm.2018.3770
MLA
Park, J., Choi, G., Yim, M., Lee, J. M., Yoo, J. S., Park, W. S., Park, S. K., Park, S., Seo, S., Kim, ., Lee, D., Choi, I."Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 42.4 (2018): 2213-2220.
Chicago
Park, J., Choi, G., Yim, M., Lee, J. M., Yoo, J. S., Park, W. S., Park, S. K., Park, S., Seo, S., Kim, ., Lee, D., Choi, I."Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 42, no. 4 (2018): 2213-2220. https://doi.org/10.3892/ijmm.2018.3770
Copy and paste a formatted citation
x
Spandidos Publications style
Park J, Choi G, Yim MJ, Lee JM, Yoo JS, Park WS, Park SK, Park S, Seo SK, Kim G, Kim G, et al: Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts. Int J Mol Med 42: 2213-2220, 2018.
APA
Park, J., Choi, G., Yim, M., Lee, J.M., Yoo, J.S., Park, W.S. ... Choi, I. (2018). Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts. International Journal of Molecular Medicine, 42, 2213-2220. https://doi.org/10.3892/ijmm.2018.3770
MLA
Park, J., Choi, G., Yim, M., Lee, J. M., Yoo, J. S., Park, W. S., Park, S. K., Park, S., Seo, S., Kim, ., Lee, D., Choi, I."Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 42.4 (2018): 2213-2220.
Chicago
Park, J., Choi, G., Yim, M., Lee, J. M., Yoo, J. S., Park, W. S., Park, S. K., Park, S., Seo, S., Kim, ., Lee, D., Choi, I."Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor β1‑induced nasal polyp‑derived fibroblasts". International Journal of Molecular Medicine 42, no. 4 (2018): 2213-2220. https://doi.org/10.3892/ijmm.2018.3770
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team