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Article Open Access

Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro

  • Authors:
    • Yu‑Wei Ge
    • Zhi‑Qing Liu
    • Zhen‑Yu Sun
    • De‑Gang Yu
    • Kai Feng
    • Zhen‑An Zhu
    • Yuan‑Qing Mao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
    Copyright: © Ge et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2031-2041
    |
    Published online on: July 17, 2018
       https://doi.org/10.3892/ijmm.2018.3780
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Abstract

The present study investigated the role of bidirectional ephrin‑B2/erythropoietin‑producing human hepatocellular receptor 4 (ephB4) signaling in the regulation of wear particle‑mediated osteoclastogenesis in vitro. Mouse bone marrow macrophages (BMMs) were induced into osteoclasts by receptor activator of nuclear factor‑κB ligand (RANKL, 50 ng/ml). EphB4‑Fc, an osteoblast membrane surface receptor (4 µg/ml), was used to stimulate the ephrin‑B2 ligand of osteoclasts in the presence and absence of titanium (Ti). Tartrate‑resistant acid phosphatase (TRAP) staining was used to detect the number of osteoclasts, and phalloidin staining was used to examine the cytoskeletons of the osteoclasts. A bone pit absorption experiment was used to measure osteoclast function. Reverse transcription quantitative polymerase chain reaction and western blot analysis were used to examine osteoclastogenesis. ELISAs were used to detect the production of inflammatory factors. The data demonstrated that Ti significantly promoted the differentiation of BMMs into mature osteoclasts in the presence of RANKL and significantly promoted expression of the ephrin‑B2, nuclear factor of activated T‑cells 1 (NFATc1), TRAP, Fos proto‑oncogene, AP‑1 transcription factor subunit (C‑FOS), and matrix metalloproteinase 9 (MMP9) genes. Phalloidin and TRAP staining revealed that following the addition of ephB4‑Fc, the number, size and cytoskeletal elements of osteoclasts were significantly decreased compared with those in the titanium particle group without ephB4‑Fc. Compared with the titanium particle group, the bone pit absorption experiment revealed significantly decreased absorption pit areas in the titanium particle+ephB4‑Fc group. The expression of the NFATc1, TRAP, C‑FOS and MMP9 genes was markedly decreased in the ephB4‑Fc group; however, the expression of the ephrin‑B2 gene was increased compared with the Ti particle group without ephB4‑Fc after 5 days. Production of inflammatory cytokines was inhibited by Ti particles through bidirectional signals. Addition of ephB4‑Fc inhibited the osteoclast‑mediated formation of Ti particles via bidirectional ephrin‑B2/ephB4 signaling. Activation of this bidirectional signaling pathway may be a potential clinical treatment for osteolysis surrounding prostheses.
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Copy and paste a formatted citation
Spandidos Publications style
Ge YW, Liu ZQ, Sun ZY, Yu DG, Feng K, Zhu ZA and Mao YQ: Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro. Int J Mol Med 42: 2031-2041, 2018.
APA
Ge, Y., Liu, Z., Sun, Z., Yu, D., Feng, K., Zhu, Z., & Mao, Y. (2018). Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro. International Journal of Molecular Medicine, 42, 2031-2041. https://doi.org/10.3892/ijmm.2018.3780
MLA
Ge, Y., Liu, Z., Sun, Z., Yu, D., Feng, K., Zhu, Z., Mao, Y."Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro". International Journal of Molecular Medicine 42.4 (2018): 2031-2041.
Chicago
Ge, Y., Liu, Z., Sun, Z., Yu, D., Feng, K., Zhu, Z., Mao, Y."Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro". International Journal of Molecular Medicine 42, no. 4 (2018): 2031-2041. https://doi.org/10.3892/ijmm.2018.3780
Copy and paste a formatted citation
x
Spandidos Publications style
Ge YW, Liu ZQ, Sun ZY, Yu DG, Feng K, Zhu ZA and Mao YQ: Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro. Int J Mol Med 42: 2031-2041, 2018.
APA
Ge, Y., Liu, Z., Sun, Z., Yu, D., Feng, K., Zhu, Z., & Mao, Y. (2018). Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro. International Journal of Molecular Medicine, 42, 2031-2041. https://doi.org/10.3892/ijmm.2018.3780
MLA
Ge, Y., Liu, Z., Sun, Z., Yu, D., Feng, K., Zhu, Z., Mao, Y."Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro". International Journal of Molecular Medicine 42.4 (2018): 2031-2041.
Chicago
Ge, Y., Liu, Z., Sun, Z., Yu, D., Feng, K., Zhu, Z., Mao, Y."Titanium particle‑mediated osteoclastogenesis may be attenuated via bidirectional ephrin‑B2/eph‑B4 signaling in vitro". International Journal of Molecular Medicine 42, no. 4 (2018): 2031-2041. https://doi.org/10.3892/ijmm.2018.3780
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