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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October-2018 Volume 42 Issue 4

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Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway

  • Authors:
    • Nan Wang
    • Zhiping Feng
    • Wei Zhao
    • Zhiqiang Zhang
    • Lixin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Rehabilitation, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110022, P.R. China, The 4th Department of Orthopedic Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1909-1916
    |
    Published online on: July 19, 2018
       https://doi.org/10.3892/ijmm.2018.3786
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Abstract

Mitogen‑activated protein kinase‑activated protein kinase 2 (MK2) and its mediated inflammation are involved in various diseases, including spinal cord injury (SCI). Ultrashortwave (USW) radiation has previously been reported to exert a protective effect on SCI. In the present study, through a series of reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blot and immunofluorescence assay, it was found that MK2 and tumor necrosis factor (TNF)‑α/interleukin (IL)‑1β were elevated in patients with SCI and in H2O2‑treated C8‑D1A cells. Through gene level and protein level detection by using of RT‑qPCR, western blot, immunofluorescence assay and terminal deoxynucleotidyl transferase (TdT) dUTP nick‑end labeling assay, it was demonstrated that USW radiation inhibited the expression of MK2/TNF‑α/IL‑1β and suppressed the apoptosis of H2O2‑treated C8‑D1A cells. Furthermore, it was confirmed that the overexpression of MK2 reversed the protective effect of USW on C8‑D1A cells, which indicated that USW achieved its function via regulation of the MK2/TNF‑α/IL‑1β pathway. Finally, using a constructed in vivo model and a series of RT‑qPCR, western blot and IHC detection, it was confirmed that USW suppressed the expression of MK2 to promote functional recovery following SCI.
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Copy and paste a formatted citation
Spandidos Publications style
Wang N, Feng Z, Zhao W, Zhang Z and Zhang L: Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway. Int J Mol Med 42: 1909-1916, 2018.
APA
Wang, N., Feng, Z., Zhao, W., Zhang, Z., & Zhang, L. (2018). Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway. International Journal of Molecular Medicine, 42, 1909-1916. https://doi.org/10.3892/ijmm.2018.3786
MLA
Wang, N., Feng, Z., Zhao, W., Zhang, Z., Zhang, L."Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway". International Journal of Molecular Medicine 42.4 (2018): 1909-1916.
Chicago
Wang, N., Feng, Z., Zhao, W., Zhang, Z., Zhang, L."Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway". International Journal of Molecular Medicine 42, no. 4 (2018): 1909-1916. https://doi.org/10.3892/ijmm.2018.3786
Copy and paste a formatted citation
x
Spandidos Publications style
Wang N, Feng Z, Zhao W, Zhang Z and Zhang L: Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway. Int J Mol Med 42: 1909-1916, 2018.
APA
Wang, N., Feng, Z., Zhao, W., Zhang, Z., & Zhang, L. (2018). Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway. International Journal of Molecular Medicine, 42, 1909-1916. https://doi.org/10.3892/ijmm.2018.3786
MLA
Wang, N., Feng, Z., Zhao, W., Zhang, Z., Zhang, L."Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway". International Journal of Molecular Medicine 42.4 (2018): 1909-1916.
Chicago
Wang, N., Feng, Z., Zhao, W., Zhang, Z., Zhang, L."Ultrashortwave radiation promotes the recovery of spinal cord injury by inhibiting inflammation via suppression of the MK2/TNF‑α pathway". International Journal of Molecular Medicine 42, no. 4 (2018): 1909-1916. https://doi.org/10.3892/ijmm.2018.3786
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