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Aspirin promotes osteogenic differentiation of human dental pulp stem cells

  • Authors:
    • Mengtong Yuan
    • Yuanbo Zhan
    • Weiping Hu
    • Ying Li
    • Xiaohua Xie
    • Nan Miao
    • Han Jin
    • Bin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Prosthodontics, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Institute of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1967-1976
    |
    Published online on: August 2, 2018
       https://doi.org/10.3892/ijmm.2018.3801
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Abstract

Human dental pulp stem cells (hDPSCs) possess self‑renewal and osteogenic differentiation properties, and have been used for orofacial bone regeneration and periodontal treatment. Aspirin has been demonstrated to enhance the regeneration of bone marrow mesenchymal stem cells (MSCs); however, the impact of aspirin on the osteogenic differentiation of hDPSCs remains unknown. In the present study, hDPSCs were characterized by flow cytometry, while their clonogenic potential and multipotency were assessed using alizarin red, Oil red O and alcian blue staining. The effect of aspirin on hDPSC viability was assessed using Cell Counting Kit‑8 assay. Osteogenic capacity was examined by alkaline phosphatase activity, alizarin red staining, reverse transcription‑polymerase chain reaction and western blotting. Furthermore, in vivo cranial defects were established in Sprague‑Dawley rats to evaluate the effect of aspirin on hDPSC‑based bone regeneration. Anorganic bovine bone was used as a bone replacement material and as the carrier for hDPSCs. New bone formation was observed through radiographic and histological analysis. The study demonstrated that hDPSCs expressed MSC markers and possessed multipotency in vitro. Aspirin was non‑toxic to hDPSCs at a concentration of ≤100 µg/ml and enhanced the osteogenesis of hDPSCs in vitro. Aspirin significantly increased hDPSC‑based bone formation in the rat cranial defect model at 8 or 12 weeks post‑implantation (P<0.05). The data suggested that aspirin promotes the osteogenic potential of hDPSCs in vitro and in vivo. Overall, the present study indicated that aspirin improves the bone regeneration capacity of hDPSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan M, Zhan Y, Hu W, Li Y, Xie X, Miao N, Jin H and Zhang B: Aspirin promotes osteogenic differentiation of human dental pulp stem cells. Int J Mol Med 42: 1967-1976, 2018.
APA
Yuan, M., Zhan, Y., Hu, W., Li, Y., Xie, X., Miao, N. ... Zhang, B. (2018). Aspirin promotes osteogenic differentiation of human dental pulp stem cells. International Journal of Molecular Medicine, 42, 1967-1976. https://doi.org/10.3892/ijmm.2018.3801
MLA
Yuan, M., Zhan, Y., Hu, W., Li, Y., Xie, X., Miao, N., Jin, H., Zhang, B."Aspirin promotes osteogenic differentiation of human dental pulp stem cells". International Journal of Molecular Medicine 42.4 (2018): 1967-1976.
Chicago
Yuan, M., Zhan, Y., Hu, W., Li, Y., Xie, X., Miao, N., Jin, H., Zhang, B."Aspirin promotes osteogenic differentiation of human dental pulp stem cells". International Journal of Molecular Medicine 42, no. 4 (2018): 1967-1976. https://doi.org/10.3892/ijmm.2018.3801
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan M, Zhan Y, Hu W, Li Y, Xie X, Miao N, Jin H and Zhang B: Aspirin promotes osteogenic differentiation of human dental pulp stem cells. Int J Mol Med 42: 1967-1976, 2018.
APA
Yuan, M., Zhan, Y., Hu, W., Li, Y., Xie, X., Miao, N. ... Zhang, B. (2018). Aspirin promotes osteogenic differentiation of human dental pulp stem cells. International Journal of Molecular Medicine, 42, 1967-1976. https://doi.org/10.3892/ijmm.2018.3801
MLA
Yuan, M., Zhan, Y., Hu, W., Li, Y., Xie, X., Miao, N., Jin, H., Zhang, B."Aspirin promotes osteogenic differentiation of human dental pulp stem cells". International Journal of Molecular Medicine 42.4 (2018): 1967-1976.
Chicago
Yuan, M., Zhan, Y., Hu, W., Li, Y., Xie, X., Miao, N., Jin, H., Zhang, B."Aspirin promotes osteogenic differentiation of human dental pulp stem cells". International Journal of Molecular Medicine 42, no. 4 (2018): 1967-1976. https://doi.org/10.3892/ijmm.2018.3801
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