Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
November-2018 Volume 42 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2018 Volume 42 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR

  • Authors:
    • Xiaofang Sui
    • Shuqian Yu
    • Lin Dou
    • Xiehui Chen
    • Xuejie Li
    • Jun Yang
    • Yanan Su
    • Shuyue Wang
    • Fengling Wang
    • Jian Li
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China, The Ministry of Health Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, P.R. China, Department of Geriatric Cardiovascular Medicine, Shenzhen Sun Yat‑Sen Cardiovascular Hospital, Shenzhen, Guangdong 518112, P.R. China, Department of Geriatrics, Clinical Medical School, Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China, National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, Jilin, P.R. China
    Copyright: © Sui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2383-2392
    |
    Published online on: August 10, 2018
       https://doi.org/10.3892/ijmm.2018.3821
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Endothelial dysfunction is an early marker of atherosclerosis. Previous studies have indicated that microRNA (miR)‑291b‑3p regulates the metabolism of lipids and glucose in the liver via targeting adenosine monophosphate‑activated kinase α1 and transcription factor p65. The present study investigated whether miR‑291b‑3p mediated H2O2‑mediated endothelial dysfunction. The level of apoptosis of EOMA mouse endothelial cells was analyzed by terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labelling staining. The mRNA levels of miR‑291b‑3p, intercellular adhesion molecule‑1 (ICAM‑1) and vascular adhesion molecule‑1 (VCAM‑1) were determined by quantitative polymerase chain reaction. The level of phosphorylated extracellular signal‑regulated kinase, and levels of B‑cell lymphoma 2 (Bcl‑2)‑associated X protein and Bcl‑2 protein were detected by western blot analysis. The treatment of H2O2 induced the apoptosis and increased the mRNA levels of miR‑291b‑3p, ICAM‑1 and VCAM‑1 in EOMA cells. It was also demonstrated that the overexpression of miR‑291b‑3p promoted EOMA cell apoptosis and dysfunction. In contrast, the downregulation of miR‑291b‑3p rescued the effect of H2O2 on EOMA cell dysfunction. In addition, Hu antigen R (HuR) was identified as a target gene of miR‑291b‑3p in EOMA cells. The overexpression of HuR reversed the endothelial dysfunction induced by miR‑291b‑3p mimics. The present study provides novel insight into the critical role of miR‑291b‑3p on the endothelial dysfunction induced by H2O2. miR‑291b‑3p may mediate H2O2‑induced endothelial dysfunction via targeting HuR.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Mannarino E and Pirro M: Molecular biology of atherosclerosis. Clin Cases Miner Bone Metab. 5:57–62. 2008.PubMed/NCBI

2 

Ghiadoni L, Taddei S and Virdis A: Hypertension and endothelial dysfunction: Therapeutic approach. Curr Vasc Pharmacol. 10:42–60. 2012. View Article : Google Scholar

3 

Yung LM, Leung FP, Yao X, Chen ZY and Huang Y: Reactive oxygen species in vascular wall. Cardiovasc Hematol Disord Drug Targets. 6:1–19. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Baumgartner R, Forteza MJ and Ketelhuth DFJ: The interplay between cytokines and the Kynurenine pathway in inflammation and atherosclerosis. Cytokine. Sep 9–2017.(Epub ahead of print). pii: S1043-4666(17)30259-4. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Marzolla V, Armani A, Mammi C, Moss ME, Pagliarini V, Pontecorvo L, Antelmi A, Fabbri A, Rosano G, Jaffe IZ and Caprio M: Essential role of ICAM-1 in aldosterone-induced atherosclerosis. Int J Cardiol. 232:233–242. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Jang YJ, Park B, Lee HW, Park HJ, Koo HJ, Kim BO, Sohn EH, Um SH and Pyo S: Sinigrin attenuates the progression of atherosclerosis in ApoE- mice fed a high-cholesterol diet potentially by inhibiting VCAM-1 expression. Chem Biol Interact. 272:28–36. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Menghini R, Stohr R and Federici M: MicroRNAs in vascular aging and atherosclerosis. Ageing Res Rev. 17:68–78. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Feinberg MW and Moore KJ: MicroRNA regulation of atherosclerosis. Circ Res. 118:703–720. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Karakas M, Schulte C, Appelbaum S, Ojeda F, Lackner KJ, Münzel T, Schnabel RB, Blankenberg S and Zeller T: Circulating microRNAs strongly predict cardiovascular death in patients with coronary artery disease-results from the large AtheroGene study. Eur Heart J. 38:516–523. 2017.

10 

Fichtlscherer S, De Rosa S, Fox H, Schwietz T, Fischer A, Liebetrau C, Weber M, Hamm CW, Röxe T, Müller-Ardogan M, et al: Circulating microRNAs in patients with coronary artery disease. Circ Res. 107:677–684. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Sun X, Icli B, Wara AK, Belkin N, He S, Kobzik L, Hunninghake GM, Vera MP, MICU Registry, Blackwell TS, Baron RM and Feinberg MW: MicroRNA-181b regulates NF-κB-mediated vascular inflammation. J Clin Invest. 122:1973–1990. 2012.PubMed/NCBI

12 

Sun X, He S, Wara AKM, Icli B, Shvartz E, Tesmenitsky Y, Belkin N, Li D, Blackwell TS, Sukhova GK, et al: Systemic delivery of microRNA-181b inhibits nuclear factor-κB activation, vascular inflammation, and atherosclerosis in apolipoprotein E-deficient mice. Circ Res. 114:32–40. 2014. View Article : Google Scholar

13 

Lin Y, Liu X, Cheng Y, Yang J, Huo Y and Zhang C: Involvement of MicroRNAs in hydrogen peroxide-mediated gene regulation and cellular injury response in vascular smooth muscle cells. J Biol Chem. 284:7903–7913. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Zhang T, Tian F, Wang J, Jing J, Zhou SS and Chen YD: Atherosclerosis-associated endothelial cell apoptosis by miR-429-mediated down regulation of Bcl-2. Cell Physiol Biochem. 37:1421–1430. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Guo J, Li M, Meng X, Sui J, Dou L, Tang W, Huang X, Man Y, Wang S and Li J: miR-291b-3p induces apoptosis in liver cell line NCTC1469 by reducing the level of RNA-binding protein HuR. Cell Physiol Biochem. 33:810–822. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Meng X, Guo J, Fang W, Dou L, Li M, Huang X, Zhou S, Man Y, Tang W, Yu L and Li J: Liver MicroRNA-291b-3p promotes hepatic lipogenesis through negative Regulation of Adenosine 5′-Monophosphate (AMP)-activated protein kinase α1. J Biol Chem. 291:10625–10634. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Guo J, Dou L, Meng X, Chen Z, Yang W, Fang W, Yang C, Huang X, Tang W, Yang J and Li J: Hepatic miR-291b-3p mediated glucose metabolism by directly targeting p65 to upregulate PTEN expression. Sci Rep. 7:398992017. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR ad the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

19 

Xu MC, Gao XF, Ruan C, Ge ZR, Lu JD, Zhang JJ, Zhang Y, Wang L and Shi HM: miR-103 regulates oxidative stress by targeting the BCL2/Adenovirus E1B 19 kDa interacting protein 3 in HUVECs. Oxid Med Cell Longev. 2015:4896472015. View Article : Google Scholar

20 

Yang B, Oo TN and Rizzo V: Lipid rafts mediate H2O2 prosurvival effects in cultured endothelial cells. FASEB J. 20:1501–1503. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Watkin RL, Fitzpatrick GG and Kerrigan SW: The evolving role of microRNAs in endothelial cell dysfunction in response to infection. Semin Thromb Hemost. 44:216–223. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Marson A, Levine SS, Cole MF, Frampton GM, Brambrink T, Johnstone S, Guenther MG, Johnston WK, Wernig M, Newman J, et al: Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells. Cell. 134:521–533. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Zheng GX, Ravi A, Calabrese JM, Medeiros LA, Kirak O, Dennis LM, Jaenisch R, Burge CB and Sharp PA: A latent pro-survival function for the mir-290-295 cluster in mouse embryonic stem cells. PLoS Genet. 7:e10020542011. View Article : Google Scholar : PubMed/NCBI

24 

Hoefen RJ and Berk BC: The role of MAP kinases in endothelial activation. Vasc Pharmacol. 38:271–273. 2002. View Article : Google Scholar

25 

Yaman I, Fernandez J, Sarkar B, Schneider RJ, Snider MD, Nagy LE and Hatzoglou M: Nutritional control of mRNA stability is mediated by a conserved AU-rich element that binds the cytoplasmic shuttling protein HuR. J Biol Chem. 277:41539–41546. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Sharma S, Verma S, Vasudevan M, Samanta S, Thakur JK and Kulshreshtha R: The interplay of HuR and miR-3134 in regulation of AU rich transcriptome. RNA Biol. 10:1283–1290. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Chen CY, Xu N and Shyu AB: Highly selective actions of HuR in antagonizing AU-rich element-mediated mRNA destabilization. Mol Cell Biol. 22:7268–7278. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Wang W, Furneaux H, Cheng H, Caldwell MC, Hutter D, Liu Y, Holbrook N and Gorospe M: HuR regulates p21 mRNA stabilization by UV light. Mol Cell Biol. 20:760–769. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Kim GY, Lim SJ and Kim YW: Expression of HuR, COX-2, and survivin in lung cancers; cytoplasmic HuR stabilizes cyclooxygenase-2 in squamous cell carcinomas. Mod Pathol. 24:1336–1347. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Yuan Z, Sanders AJ, Ye L, Wang Y and Jiang WG: Knockdown of human antigen R reduces the growth and invasion of breast cancer cells in vitro and affects expression of cyclin D1 and MMP-9. Oncol Rep. 26:237–245. 2011.PubMed/NCBI

31 

Nabors LB, Suswam E, Huang Y, Yang X, Johnson MJ and King PH: Tumor necrosis factor alpha induces angiogenic factor up-regulation in malignant glioma cells: A role for RNA stabilization and HuR. Cancer Res. 63:4181–4187. 2003.PubMed/NCBI

32 

Talwar S, Jin J, Carroll B, Liu A, Gillespie MB and Palanisamy V: Caspase-mediated cleavage of RNA-binding protein HuR regulates c-Myc protein expression after hypoxic stress. J Biol Chem. 286:32333–32343. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Kullmann M, Göpfert U, Siewe B and Hengst L: ELAV/Hu proteins inhibit p27 translation via an IRES element in the p27 5′UTR. Genes Dev. 16:3087–3099. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sui X, Yu S, Dou L, Chen X, Li X, Yang J, Su Y, Wang S, Wang F, Li J, Li J, et al: miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR. Int J Mol Med 42: 2383-2392, 2018.
APA
Sui, X., Yu, S., Dou, L., Chen, X., Li, X., Yang, J. ... Li, J. (2018). miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR. International Journal of Molecular Medicine, 42, 2383-2392. https://doi.org/10.3892/ijmm.2018.3821
MLA
Sui, X., Yu, S., Dou, L., Chen, X., Li, X., Yang, J., Su, Y., Wang, S., Wang, F., Li, J."miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR". International Journal of Molecular Medicine 42.5 (2018): 2383-2392.
Chicago
Sui, X., Yu, S., Dou, L., Chen, X., Li, X., Yang, J., Su, Y., Wang, S., Wang, F., Li, J."miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR". International Journal of Molecular Medicine 42, no. 5 (2018): 2383-2392. https://doi.org/10.3892/ijmm.2018.3821
Copy and paste a formatted citation
x
Spandidos Publications style
Sui X, Yu S, Dou L, Chen X, Li X, Yang J, Su Y, Wang S, Wang F, Li J, Li J, et al: miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR. Int J Mol Med 42: 2383-2392, 2018.
APA
Sui, X., Yu, S., Dou, L., Chen, X., Li, X., Yang, J. ... Li, J. (2018). miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR. International Journal of Molecular Medicine, 42, 2383-2392. https://doi.org/10.3892/ijmm.2018.3821
MLA
Sui, X., Yu, S., Dou, L., Chen, X., Li, X., Yang, J., Su, Y., Wang, S., Wang, F., Li, J."miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR". International Journal of Molecular Medicine 42.5 (2018): 2383-2392.
Chicago
Sui, X., Yu, S., Dou, L., Chen, X., Li, X., Yang, J., Su, Y., Wang, S., Wang, F., Li, J."miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR". International Journal of Molecular Medicine 42, no. 5 (2018): 2383-2392. https://doi.org/10.3892/ijmm.2018.3821
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team