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Article Open Access

Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg

Corrigendum in: /10.3892/ijmm.2019.4055
  • Authors:
    • Tong Wang
    • Yuzhu Dai
    • Meng Zhang
    • Dawei Cui
    • Xujian Xu
    • Changgui Sun
    • Jun Cheng
  • View Affiliations / Copyright

    Affiliations: Faculty of Graduate Studies, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Clinical Laboratory, The 117th Hospital of PLA, Hangzhou, Zhejiang 310013, P.R. China, Department of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Biotechnology, The University of Tokyo, Tokyo 1138656, Japan
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2689-2699
    |
    Published online on: August 17, 2018
       https://doi.org/10.3892/ijmm.2018.3831
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Abstract

In a hepatitis B virus (HBV)‑infected population, persistently low expression levels of serum HBV serum antigen (HBsAg) are present, particularly in chronic asymptomatic HBV carriers (ASCs). The present study sequenced the HBV Pre‑S gene, and aimed to elucidate its features in ASCs with low HBsAg expression compared with in the established HBV Pre‑S reference gene sequences from ASCs with high HBsAg expression. A total of 1,308 ASCs were grouped according to HBsAg serum levels (cut‑off value, 10 IU/ml), and clinical characteristics were analyzed in detail. The HBV Pre‑S gene was sequenced in 276 ASCs with low‑level HBsAg; in addition, 100 of the remaining 1,032 ASCs with high‑level HBsAg were randomly selected for HBV Pre‑S gene sequencing on the basis of age matching with the low‑level HBsAg group. Comparative analysis of the gene sequences from these groups was subsequently conducted. The major clinical features of the population with low‑level HBsAg were as follows: Most were ASCs with chronic HBV infection; 97.1% were HBsAg/anti‑HBe/anti‑HBc‑positive; 82.54% carried the B genotype; and 84.13% displayed the adw serotype. The results indicated that there were novel and meaningful mutations, including co‑mutations, at numerous loci and sites in the Pre‑S gene, as well as deletion mutations in the Pre‑S2 gene. These mutations in the Pre‑S1 and Pre‑S2 gene fragments accounted for 65.38% (68/104) of the 104 B genotype cases in the low‑level HBsAg group and 90.91% (20/22) of the 22 C genotype cases in the low‑level HBsAg group, respectively. In conclusion, Pre‑S gene mutations may be associated with HBV replication defects, which may be the cause of the observed low expression levels of HBsAg.
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View References

1 

Ott JJ, Horn J, Krause G and Mikolajczyk RT: Time trends of chronic HBV infection over prior decades-A global analysis. J Hepatol. 66:48–54. 2017. View Article : Google Scholar

2 

Xiang KH, Michailidis E, Ding H, Peng YQ, Su MZ, Li Y, Liu XE, Dao Thi VL, Wu XF, Schneider WM, et al: Effects of amino acid substitutions in hepatitis B virus surface protein on virion secretion, antigenicity, HBsAg and viral DNA. J Hepatol. 66:288–296. 2017. View Article : Google Scholar :

3 

Pollicino T, Amaddeo G, Restuccia A, Raffa G, Alibrandi A, Cutroneo G, Favaloro A, Maimone S, Squadrito G and Raimondo G: Impact of hepatitis B virus (HBV) preS/S genomic variability on HBV surface antigen and HBV DNA serum levels. Hepatology. 56:434–443. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Zhang ZH, Wu CC, Chen XW, Li X, Li J and Lu MJ: Genetic variation of hepatitis B virus and its significance for pathogenesis. World J Gastroenterol. 22:126–144. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Chen CH, Changchien CS, Lee CM, Tung WC, Hung CH, Hu TH, Wang JH, Wang JC and Lu SN: A study on sequence variations in pre-S/surface, X and enhancer II/core promoter/precore regions of occult hepatitis B virus in non-B, non-C hepatocellular carcinoma patients in Taiwan. Int J Cancer. 125:621–629. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Cento V, Van Hemert F, Neumann-Fraune M, Mirabelli C, Di Maio VC, Salpini R, Bertoli A, Micheli V, Gubertini G, Romano S, et al: Anti-HBV treatment induces novel reverse transcriptase mutations with reflective effect on HBV S antigen. J Infect. 67:303–312. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Kim H, Lee SA, Won YS, Lee H and Kim BJ: Occult infection related hepatitis B surface antigen variants showing lowered secretion capacity. World J Gastroenterol. 21:1794–1803. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Kim BK, Choi SH, Ahn SH, Chung AR, Park YK, Han KH, Kim S, Kim HS, Park JH, Kim KS, et al: Pre-S mutations of hepatitis B virus affect genome replication and expression of surface antigens. J Gastroenterol Hepatol. 29:843–850. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Chinese Society of Hepatology, Chinese Medical Association; Chinese Society of Infectious Diseases, Chinese Medical Association; Hou JL and lai W: The guideline of prevention and treatment for chronic hepatitis B: A 2015 update. Zhonghua Gan Zang Bing Za Zhi. 23:888–905. 2015.In Chinese.

10 

European Association for the Study of the Liver. Electronic address: simpleeasloffice@easloffice.eu; European Association for the Study of the Liver: EASL 2017 Clinical Practice Guidelines on the management of hepatitis B virus infection. J Hepatol. 67:370–398. 2017. View Article : Google Scholar

11 

Guidelines for the Prevention: Care and treatment of persons with chronic hepatitis B infection WHO guidelines approved by the guidelines review committee. World Health Organization; Geneva: 2015

12 

Kumar M, Sarin SK, Hissar S, Pande C, Sakhuja P, Sharma BC, Chauhan R and Bose S: Virologic and histologic features of chronic hepatitis B virus-infected asymptomatic patients with persistently normal ALT. Gastroenterology. 134:1376–1384. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Chen Y and Wu W: Determination of low level HBsAg in serum by microparticle enzyme immunoassay. Hepatobiliary Pancreat Dis Int. 1:262–264. 2002.

14 

Cheng J, Sun CG, Chen Y, Xu ZL, Dai YZ, Sun GZ and Li XJ: Relationship between the quantitative results of HBV DNA and HBV markers (HBV M) in population with low-level HBsAg. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 25:631–633. 6362009.In Chinese.

15 

Chook JB, Teo WL, Ngeow YF, Tee KK, Ng KP and Mohamed R: Universal primers for detection and sequencing of hepatitis B virus genomes across genotypes A to G. J Clin Microbiol. 53:1831–1835. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Tamura K, Stecher G, Peterson D, Filipski A and Kumar S: MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 30:2725–2729. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Liao H, Liu Y, Chen J, Ding W, Li X, Xu Z, Yang Y, Chen R, Si L, Xu X, et al: Characterization of hepatitis B virus (HBV) preS/S gene mutations in blood donors with occult HBV infection in the Baoji area of North China. Transfusion. 57:857–866. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Tong S and Revill P: Overview of hepatitis B viral replication and genetic variability. J Hepatol. 64(Suppl 1): S4–S16. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Norder H, Couroucé AM, Coursaget P, Echevarria JM, Lee SD, Mushahwar IK, Robertson BH, Locarnini S and Magnius LO: Genetic diversity of hepatitis B virus strains derived worldwide: Genotypes, subgenotypes, and HBsAg subtypes. Intervirology. 47:289–309. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Taylor SM, Parobek CM, DeConti DK, Kayentao K, Coulibaly SO, Greenwood BM, Tagbor H, Williams J, Bojang K, Njie F, et al: Absence of putative artemisinin resistance mutations among Plasmodium falciparum in Sub-Saharan Africa: A molecular epidemiologic study. J Infect Dis. 211:680–688. 2015. View Article : Google Scholar :

21 

Zhong YW, Di FL, Liu C, Zhang XC, Bi JF, Li YL, Wu SQ, Dong H, Liu LM, He J, et al: Hepatitis B virus basal core promoter/precore mutants and association with liver cirrhosis in children with chronic hepatitis B virus infection. Clin Microbiol Infect. 22:379.e1–379.e8. 2016. View Article : Google Scholar

22 

Yin J, Xie J, Zhang H, Shen Q, Han L, Lu W, Han Y, Li C, Ni W, Wang H and Cao G: Significant association of different preS mutations with hepatitis B-related cirrhosis or hepatocellular carcinoma. J Gastroenterol. 45:1063–1071. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Martinot-Peignoux M and Marcellin P: Virological and sero-logical tools to optimize the management of patients with chronic hepatitis B. Liver Int. 36(Suppl 1): S78–S84. 2016. View Article : Google Scholar

24 

Jaroszewicz J, Calle Serrano B, Wursthorn K, Deterding K, Schlue J, Raupach R, Flisiak R, Bock CT, Manns MP, Wedemeyer H and Cornberg M: Hepatitis B surface antigen (HBsAg) levels in the natural history of hepatitis B virus (HBV)-infection: A European perspective. J Hepatol. 52:514–522. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Nguyen T, Thompson AJ, Bowden S, Croagh C, Bell S, Desmond PV, Levy M and Locarnini SA: Hepatitis B surface antigen levels during the natural history of chronic hepatitis B: A perspective on Asia. J Hepatol. 52:508–513. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Karra VK, Chowdhury SJ, Ruttala R, Polipalli SK and Kar P: Clinical significance of quantitative HBsAg titres and its correlation with HBV DNA levels in the natural history of hepatitis B virus infection. J Clin Exp Hepatol. 6:209–215. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Chen J, Liu Y, Zhao J, Xu Z, Chen R, Si L, Lu S, Li X, Wang S, Zhang K, et al: Characterization of novel hepatitis B virus PreS/S-Gene mutations in a patient with occult hepatitis B virus infection. PLoS One. 11:e01556542016. View Article : Google Scholar : PubMed/NCBI

28 

Cai Q, Zhu H, Zhang Y, Li X and Zhang Z: Hepatitis B virus genotype A: Design of reference sequences for sub-genotypes. Virus Genes. 52:325–333. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Osiowy C: Detection of HBsAg mutants. J Med Virol. 78(Suppl 1): S48–S51. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Wang ML and Tang H: Nucleos(t)ide analogues causes HBV S gene mutations and carcinogenesis. Hepatobiliary Pancreat Dis Int. 15:579–586. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Salpini R, Colagrossi L, Bellocchi MC, Surdo M, Becker C, Alteri C, Aragri M, Ricciardi A, Armenia D, Pollicita M, et al: Hepatitis B surface antigen genetic elements critical for immune escape correlate with hepatitis B virus reactivation upon immu-nosuppression. Hepatology. 61:823–833. 2015. View Article : Google Scholar

32 

Graumann F, Churin Y, Tschuschner A, Reifenberg K, Glebe D, Roderfeld M and Roeb E: Genomic methylation inhibits expression of hepatitis B virus envelope protein in transgenic mice: A non-infectious mouse model to study silencing of HBV surface antigen genes. PLoS One. 10:e01460992015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang T, Dai Y, Zhang M, Cui D, Xu X, Sun C and Cheng J: Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055. Int J Mol Med 42: 2689-2699, 2018.
APA
Wang, T., Dai, Y., Zhang, M., Cui, D., Xu, X., Sun, C., & Cheng, J. (2018). Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055. International Journal of Molecular Medicine, 42, 2689-2699. https://doi.org/10.3892/ijmm.2018.3831
MLA
Wang, T., Dai, Y., Zhang, M., Cui, D., Xu, X., Sun, C., Cheng, J."Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055". International Journal of Molecular Medicine 42.5 (2018): 2689-2699.
Chicago
Wang, T., Dai, Y., Zhang, M., Cui, D., Xu, X., Sun, C., Cheng, J."Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055". International Journal of Molecular Medicine 42, no. 5 (2018): 2689-2699. https://doi.org/10.3892/ijmm.2018.3831
Copy and paste a formatted citation
x
Spandidos Publications style
Wang T, Dai Y, Zhang M, Cui D, Xu X, Sun C and Cheng J: Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055. Int J Mol Med 42: 2689-2699, 2018.
APA
Wang, T., Dai, Y., Zhang, M., Cui, D., Xu, X., Sun, C., & Cheng, J. (2018). Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055. International Journal of Molecular Medicine, 42, 2689-2699. https://doi.org/10.3892/ijmm.2018.3831
MLA
Wang, T., Dai, Y., Zhang, M., Cui, D., Xu, X., Sun, C., Cheng, J."Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055". International Journal of Molecular Medicine 42.5 (2018): 2689-2699.
Chicago
Wang, T., Dai, Y., Zhang, M., Cui, D., Xu, X., Sun, C., Cheng, J."Sequence analysis of the Pre-S gene in chronic asymptomatic HBV carriers with low-level HBsAg Corrigendum in /10.3892/ijmm.2019.4055". International Journal of Molecular Medicine 42, no. 5 (2018): 2689-2699. https://doi.org/10.3892/ijmm.2018.3831
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