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(-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity

  • Authors:
    • Tetsu Kawabata
    • Takanobu Otsuka
    • Kazuhiko Fujita
    • Go Sakai
    • Rie Matsushima‑Nishiwaki
    • Osamu Kozawa
    • Haruhiko Tokuda
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8601, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan
    Copyright: © Kawabata et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3149-3156
    |
    Published online on: September 18, 2018
       https://doi.org/10.3892/ijmm.2018.3884
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Abstract

Phenolic compounds provide health benefits in humans. A previous study by our group has indicated that the epidermal growth factor (EGF)‑induced migration of osteoblast‑like MC3T3‑E1 cells is mediated by the phosphorylation of p44/p42 mitogen‑activated protein (MAPK), p38 MAPK, stress‑activated protein kinase (SAPK)/c‑Jun N‑terminal kinase (JNK) and Akt, and that resveratrol, a major polyphenol in grape skin, suppresses the EGF‑induced migration by attenuating Akt and SAPK/JNK activation. In the present study, the effects of chlorogenic acid, a major phenolic acid in coffee, and (‑)‑epigallocatechin gallate (EGCG), a major flavonoid in green tea, on the EGF‑induced migration of MC3T3‑E1 cells were investigated. EGCG significantly reduced the EGF‑induced migration as evaluated by a Transwell migration assay and by a wound healing assay. However, chlorogenic acid failed to affect the EGF‑induced migration. The phosphorylation of p38 MAPK induced by EGF was significantly suppressed by EGCG; however, the EGF‑induced phosphorylation of p44/p42 MAP kinase, SAPK/JNK or Akt was not affected by EGCG. These results suggest that EGCG, but not chlorogenic acid, suppresses EGF‑induced osteoblast migration through inhibiting p38 MAPK activation.
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Copy and paste a formatted citation
Spandidos Publications style
Kawabata T, Otsuka T, Fujita K, Sakai G, Matsushima‑Nishiwaki R, Kozawa O and Tokuda H: (-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity. Int J Mol Med 42: 3149-3156, 2018.
APA
Kawabata, T., Otsuka, T., Fujita, K., Sakai, G., Matsushima‑Nishiwaki, R., Kozawa, O., & Tokuda, H. (2018). (-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity. International Journal of Molecular Medicine, 42, 3149-3156. https://doi.org/10.3892/ijmm.2018.3884
MLA
Kawabata, T., Otsuka, T., Fujita, K., Sakai, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity". International Journal of Molecular Medicine 42.6 (2018): 3149-3156.
Chicago
Kawabata, T., Otsuka, T., Fujita, K., Sakai, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity". International Journal of Molecular Medicine 42, no. 6 (2018): 3149-3156. https://doi.org/10.3892/ijmm.2018.3884
Copy and paste a formatted citation
x
Spandidos Publications style
Kawabata T, Otsuka T, Fujita K, Sakai G, Matsushima‑Nishiwaki R, Kozawa O and Tokuda H: (-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity. Int J Mol Med 42: 3149-3156, 2018.
APA
Kawabata, T., Otsuka, T., Fujita, K., Sakai, G., Matsushima‑Nishiwaki, R., Kozawa, O., & Tokuda, H. (2018). (-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity. International Journal of Molecular Medicine, 42, 3149-3156. https://doi.org/10.3892/ijmm.2018.3884
MLA
Kawabata, T., Otsuka, T., Fujita, K., Sakai, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity". International Journal of Molecular Medicine 42.6 (2018): 3149-3156.
Chicago
Kawabata, T., Otsuka, T., Fujita, K., Sakai, G., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."(-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity". International Journal of Molecular Medicine 42, no. 6 (2018): 3149-3156. https://doi.org/10.3892/ijmm.2018.3884
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