Open Access

miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2

  • Authors:
    • Zhen Yang
    • Rui Li
    • Jun Ao
    • Qing‑De Wa
    • Yi Zhang
    • Long Chen
    • Jing Wen
    • Biao Chen
    • Wei Pan
    • Bo Li
    • Xiao‑Bin Tian
  • View Affiliations

  • Published online on: September 21, 2018     https://doi.org/10.3892/ijmm.2018.3891
  • Pages: 3115-3124
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRs) are involved in several physiological processes, including chondrogenic differentiation, however, their expression and roles in the chondrogenic differentiation of human adipose‑derived stem cells (hADSCs) remain to be fully elucidated to date. Our previous study showed that miR‑1307‑3p was significantly downregulated during chondrogenic differentiation by microarray and northern blot analysis. The present study aimed to investigate the effects of miR‑1307‑3p on chondrogenic differentiation and the underlying mechanisms. First, the decreased expression of miR‑1307‑3p was confirmed by reverse transcription‑quantitative polymerase chain reaction analysis. Subsequently, gain‑ and loss‑of‑function of miR‑1307‑3p experiments showed that the overexpression of miR‑1307‑3p suppressed the deposition of cartilage matrix proteoglycans and decreased the expression of cartilage‑related markers, including sex determining region Y‑box 9, collagen type II α1 chain and aggrecan, whereas the knockdown of miR‑1307‑3p had the opposite effect. In addition, bone morphogenetic protein receptor type 2 (BMPR2) was identified as a target of miR‑1307‑3p. Further mechanistic investigations showed that miR‑1307‑3p attenuated the chondrogenic differentiation of hADSCs at least partly by inhibiting BMPR2‑mothers against decapentaplegic signaling pathways. In conclusion, the findings revealed that miR‑1307‑3p inhibited the chondrogenic differentiation of hADSCs by targeting BMPR2 and its downstream signaling pathway, which may provide novel therapeutic clues for the treatment of cartilage injury.
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December-2018
Volume 42 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang Z, Li R, Ao J, Wa QD, Zhang Y, Chen L, Wen J, Chen B, Pan W, Li B, Li B, et al: miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. Int J Mol Med 42: 3115-3124, 2018.
APA
Yang, Z., Li, R., Ao, J., Wa, Q., Zhang, Y., Chen, L. ... Tian, X. (2018). miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. International Journal of Molecular Medicine, 42, 3115-3124. https://doi.org/10.3892/ijmm.2018.3891
MLA
Yang, Z., Li, R., Ao, J., Wa, Q., Zhang, Y., Chen, L., Wen, J., Chen, B., Pan, W., Li, B., Tian, X."miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2". International Journal of Molecular Medicine 42.6 (2018): 3115-3124.
Chicago
Yang, Z., Li, R., Ao, J., Wa, Q., Zhang, Y., Chen, L., Wen, J., Chen, B., Pan, W., Li, B., Tian, X."miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2". International Journal of Molecular Medicine 42, no. 6 (2018): 3115-3124. https://doi.org/10.3892/ijmm.2018.3891