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Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway

  • Authors:
    • Ping Dong
    • Xiyan Zhang
    • Jian Zhao
    • Dongliang Li
    • Liang Li
    • Bo Yang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Anesthesiology, The People's Hospital of Chiping, Chiping, Shandong 252100, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3238-3246
    |
    Published online on: September 25, 2018
       https://doi.org/10.3892/ijmm.2018.3895
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Abstract

In the present study, the mechanisms underlying the protective effects of microRNA‑132 (miRNA‑132) on sevoflurane‑induced neuronal apoptosis were investigated. Reverse transcription‑quantitative polymerase chain reaction and gene microarray hybridization were used to analyze alterations in microRNA levels. Cell viability, apoptosis and caspase‑3/9 activity were measured using MTT, flow cytometry and caspase‑3/9 activity kits. Immunofluorescence staining and western blot analysis were used to measure protein expression of phosphoinositide 3‑kinase (PI3K) and phosphorylated (p‑)AKT, forkhead box O3a (FOXO3a). In sevoflurane‑induced rats, the expression of miRNA‑132 was downregulated, compared with that in negative control rats. The downregulation of miRNA‑132 increased neuronal apoptosis and the upregulation of miRNA‑132 inhibited neuronal apoptosis in the sevoflurane‑induced in vitro model. The downregulation of miRNA‑132 suppressed the protein expression of PI3K and p‑AKT, and suppressed the protein expression of FOXO3a in the sevoflurane‑induced in vitro model. The PI3K inhibitor increased the effects of anti‑miRNA‑132 on neuronal apoptosis through the AKT/FOXO3a pathway in the sevoflurane‑induced in vitro model. The promotion of FOXO3a inhibited the effects of anti‑miRNA‑132 on neuronal apoptosis through the AKT/FOXO3a pathway in the sevoflurane‑induced in vitro model. These data suggested that miRNA‑132 caused sevoflurane‑induced neuronal apoptosis via suppression of the PI3K/AKT/FOXO3a pathway.
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View References

1 

Tian Y, Guo S, Wu X, Ma L and Zhao X: Minocycline alleviates sevoflurane-induced cognitive impairment in aged rats. Cell Mol Neurobiol. 35:585–594. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Yang ZJ, Wang YW, Li CL, Ma LQ and Zhao X: Pre-treatment with a Xingnaojing preparation ameliorates sevoflurane-induced neuroapoptosis in the infant rat striatum. Mol Med Rep. 11:1615–1622. 2015. View Article : Google Scholar

3 

Yufune S, Satoh Y, Akai R, Yoshinaga Y, Kobayashi Y, Endo S and Kazama T: Suppression of ERK phosphorylation through oxidative stress is involved in the mechanism underlying sevoflurane-induced toxicity in the developing brain. Sci Rep. 6:218592016. View Article : Google Scholar : PubMed/NCBI

4 

Tian Y, Wu X, Guo S, Ma L, Huang W and Zhao X: Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2. Int J Mol Med. 39:869–878. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Xiang J, Pan J, Chen F, Zheng L, Chen Y, Zhang S and Feng W: L-3-n-butylphthalide improves cognitive impairment of APP/PS1 mice by BDNF/TrkB/PI3K/AKT pathway. Int J Clin Exp Med. 7:1706–1713. 2014.PubMed/NCBI

6 

Zhu G, Wang X, Wu S and Li Q: Involvement of activation of PI3K/Akt pathway in the protective effects of puerarin against MPP+-induced human neuroblastoma SH-SY5Y cell death. Neurochem Int. 60:400–408. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Hossini AM, Quast AS, Plötz M, Grauel K, Exner T, Küchler J, Stachelscheid H, Eberle J, Rabien A, Makrantonaki E and Zouboulis CC: PI3K/AKT signaling pathway is essential for survival of induced pluripotent stem cells. PLoS One. 11:e01547702016. View Article : Google Scholar : PubMed/NCBI

8 

Liao R, Yan F, Zeng Z, Farhan M, Little P, Quirion R, Srivastava LK and Zheng W: Amiodarone-induced retinal neuronal cell apoptosis attenuated by IGF-1 via counter regulation of the PI3k/Akt/FoxO3a pathway. Mol Neurobiol. 54:6931–6943. 2017. View Article : Google Scholar

9 

Chmielarz P, Konovalova J, Najam SS, Alter H, Piepponen TP, Erfle H, Sonntag KC, Schütz G, Vinnikov IA and Domanskyi A: Dicer and microRNAs protect adult dopamine neurons. Cell Death Dis. 8:e28132017. View Article : Google Scholar : PubMed/NCBI

10 

Yoo HI, Kim BK and Yoon SK: MicroRNA-3305p negatively regulates ITGA5 expression in human colorectal cancer. Oncol Rep. 36:3023–3029. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Wang H, Li XT, Wu C, Wu ZW, Li YY, Yang TQ, Chen GL, Xie XS, Huang YL, Du ZW and Zhou YX: miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro. Onco Targets Ther. 8:3211–3218. 2015.PubMed/NCBI

12 

Leinders M, Üçeyler N, Pritchard RA, Sommer C and Sorkin LS: Increased miR-1323p expression is associated with chronic neuropathic pain. Exp Neurol. 283:276–286. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Yao C, Shi X, Zhang Z, Zhou S, Qian T, Wang Y, Ding F, Gu X and Yu B: Hypoxia-induced upregulation of miR-132 promotes schwann cell migration after sciatic nerve injury by targeting PRKAG3. Mol Neurobiol. 53:5129–5139. 2016. View Article : Google Scholar

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

15 

Zhou ZB, Yang XY, Tang Y, Zhou X, Zhou LH and Feng X: Subclinical concentrations of sevoflurane reduce oxidative stress but do not prevent hippocampal apoptosis. Mol Med Rep. 14:721–727. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Guo XQ, Cao YL, Hao F, Yan ZR, Wang ML and Liu XW: Tangeretin alters neuronal apoptosis and ameliorates the severity of seizures in experimental epilepsy-induced rats by modulating apoptotic protein expressions, regulating matrix metalloproteinases, and activating the PI3K/Akt cell survival pathway. Adv Med Sci. 62:246–253. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Luo T, Liu G, Ma H, Lu B, Xu H, Wang Y, Wu J, Ge P and Liang J: Inhibition of autophagy via activation of PI3K/Akt pathway contributes to the protection of ginsenoside Rb1 against neuronal death caused by ischemic insults. Int J Mol Sci. 15:15426–15442. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Lian R, Lu B, Jiao L, Li S, Wang H, Miao W and Yu W: MiR-132 plays an oncogenic role in laryngeal squamous cell carcinoma by targeting FOXO1 and activating the PI3K/AKT pathway. Eur J Pharmacol. 792:1–6. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Zhang Y, Han B, He Y, Li D, Ma X, Liu Q and Hao J: MicroRNA-132 attenuates neurobehavioral and neuropathological changes associated with intracerebral hemorrhage in mice. Neurochem Int. 107:182–190. 2017. View Article : Google Scholar

20 

Meng Y, Wang W, Kang J, Wang X and Sun L: Role of the PI3K/AKT signalling pathway in apoptotic cell death in the cerebral cortex of streptozotocin-induced diabetic rats. Exp Ther Med. 13:2417–2422. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Qi L and Zhang Y: The microRNA 132 regulates fluid shear stress-induced differentiation in periodontal ligament cells through mTOR signaling pathway. Cell Physiol Biochem. 33:433–445. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Zeng Z, Wang X, Bhardwaj SK, Zhou X, Little PJ, Quirion R, Srivastava LK and Zheng W: The atypical antipsychotic agent, clozapine, protects against corticosterone-induced death of PC12 cells by regulating the Akt/FoxO3a signaling pathway. Mol Neurobiol. 54:3395–3406. 2017. View Article : Google Scholar

23 

Liu MH, Yuan C, He J, Tan TP, Wu SJ, Fu HY, Liu J, Yu S, Chen YD, Le QF, et al: Resveratrol protects PC12 cells from high glucose-induced neurotoxicity via PI3K/Akt/FoxO3a pathway. Cell Mol Neurobiol. 35:513–522. 2015. View Article : Google Scholar

24 

Zhu W, Bijur GN, Styles NA and Li X: Regulation of FOXO3a by brain-derived neurotrophic factor in differentiated human SH-SY5Y neuroblastoma cells. Brain Res Mol Brain Res. 126:45–56. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Kim HY, Kwon HY, Ha Thi HT, Lee HJ, Kim GI, Hahm KB and Hong S: MicroRNA-132 and microRNA-223 control positive feedback circuit by regulating FOXO3a in inflammatory bowel disease. J Gastroenterol Hepatol. 31:1727–1735. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Dong P, Zhang X, Zhao J, Li D, Li L and Yang B: Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway. Int J Mol Med 42: 3238-3246, 2018.
APA
Dong, P., Zhang, X., Zhao, J., Li, D., Li, L., & Yang, B. (2018). Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway. International Journal of Molecular Medicine, 42, 3238-3246. https://doi.org/10.3892/ijmm.2018.3895
MLA
Dong, P., Zhang, X., Zhao, J., Li, D., Li, L., Yang, B."Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway". International Journal of Molecular Medicine 42.6 (2018): 3238-3246.
Chicago
Dong, P., Zhang, X., Zhao, J., Li, D., Li, L., Yang, B."Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway". International Journal of Molecular Medicine 42, no. 6 (2018): 3238-3246. https://doi.org/10.3892/ijmm.2018.3895
Copy and paste a formatted citation
x
Spandidos Publications style
Dong P, Zhang X, Zhao J, Li D, Li L and Yang B: Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway. Int J Mol Med 42: 3238-3246, 2018.
APA
Dong, P., Zhang, X., Zhao, J., Li, D., Li, L., & Yang, B. (2018). Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway. International Journal of Molecular Medicine, 42, 3238-3246. https://doi.org/10.3892/ijmm.2018.3895
MLA
Dong, P., Zhang, X., Zhao, J., Li, D., Li, L., Yang, B."Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway". International Journal of Molecular Medicine 42.6 (2018): 3238-3246.
Chicago
Dong, P., Zhang, X., Zhao, J., Li, D., Li, L., Yang, B."Anti-microRNA-132 causes sevoflurane‑induced neuronal apoptosis via the PI3K/AKT/FOXO3a pathway". International Journal of Molecular Medicine 42, no. 6 (2018): 3238-3246. https://doi.org/10.3892/ijmm.2018.3895
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