Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway

  • Authors:
    • Ming Ding
    • Peng Shu
    • Shuang Gao
    • Fenglou Wang
    • Yitian Gao
    • Yu Chen
    • Wenjuan Deng
    • Gaiying He
    • Zhenlin Hu
    • Tianduo Li
  • View Affiliations

  • Published online on: September 26, 2018     https://doi.org/10.3892/ijmm.2018.3901
  • Pages: 3571-3581
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Abstract

Schisandrin B (Sch B), an active extract of Schisandra chinensis, has demonstrated antioxidant activity in a number of in vitro and in vivo models. In the present study, the capacity of Sch B to protect against oxidative injury in keratinocytes using the human keratinocyte‑derived HaCaT cell line was investigated. To induce oxidative injury, tert‑Butyl hydroperoxide (tBHP) was employed. The results indicate that Sch B efficiently reduced tBHP‑induced cell death, reactive oxygen species (ROS) generation, protein oxidation, lipid peroxidation and DNA damage. Sch B also effectively attenuated the loss of mitochondrial membrane potential (MMP), and restored adenosine triphosphate (ATP) levels in tBHP‑injured HaCaT cells. Furthermore, Sch B enhanced the expression of key antioxidant enzymes, including catalase, heme oxygenase‑1, glutathione peroxidase, and superoxide dismutase, and further engaged the nuclear factor‑erythroid 2‑related factor 2 (Nrf2) signaling pathway by modulating its phosphorylation through activating multiple upstream kinases, including protein kinase B, adenosine monophosphate‑activated protein kinase and mitogen‑activated protein kinases (MAPKs). The present study suggests that Sch B provides a protective effect in keratinocytes in response to oxidative injury via reinforcing the endogenous antioxidant defense system. Therefore, it may be applied as an adjuvant therapy or in health foods to delay the skin aging process and the onset of skin diseases caused by oxidative stress.
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December-2018
Volume 42 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ding M, Shu P, Gao S, Wang F, Gao Y, Chen Y, Deng W, He G, Hu Z, Li T, Li T, et al: Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway. Int J Mol Med 42: 3571-3581, 2018
APA
Ding, M., Shu, P., Gao, S., Wang, F., Gao, Y., Chen, Y. ... Li, T. (2018). Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway. International Journal of Molecular Medicine, 42, 3571-3581. https://doi.org/10.3892/ijmm.2018.3901
MLA
Ding, M., Shu, P., Gao, S., Wang, F., Gao, Y., Chen, Y., Deng, W., He, G., Hu, Z., Li, T."Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway". International Journal of Molecular Medicine 42.6 (2018): 3571-3581.
Chicago
Ding, M., Shu, P., Gao, S., Wang, F., Gao, Y., Chen, Y., Deng, W., He, G., Hu, Z., Li, T."Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway". International Journal of Molecular Medicine 42, no. 6 (2018): 3571-3581. https://doi.org/10.3892/ijmm.2018.3901