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International Journal of Molecular Medicine
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December-2018 Volume 42 Issue 6

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MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1

  • Authors:
    • Yong Yang
    • Xiao‑Jia Li
    • Peng Li
    • Xiu‑Tian Guo
  • View Affiliations / Copyright

    Affiliations: Department of Anus and Intestine, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, P.R. China, College of Clinical Medicine, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3171-3180
    |
    Published online on: October 1, 2018
       https://doi.org/10.3892/ijmm.2018.3904
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Abstract

Primary colon adenocarcinoma is responsible for high rates of mortality worldwide. The late diagnosis and lack of reliable biomarkers and therapeutic targets forms a bottleneck in the treatment of colon cancer. In the present study, the therapeutic potential of an important microRNA (miR), namely miR‑145, was investigated in primary colon adenocarcinoma cells. The results revealed that the expression of miR‑145 was significantly (P<0.05) downregulated in colon adenocarcinoma cells and the ectopic expression of miR‑145 in colon cancer inhibited proliferation by promoting the apoptosis of SW480 primary colon adenocarcinoma cells. Furthermore, bioinformatics analysis revealed that miR‑145 exerts its effected by targeting mitogen‑activated protein kinase (MAPK) in SW480 cells. This was confirmed by expression analysis wherein the expression of MAPK1 was significantly (P<0.05) upregulated in the primary colon adenocarcinoma cells and the ectopic expression of miR‑145 inhibited the expression of MAPK1. By contrast, the silencing of MAPK1 had similar effects on the proliferation, migration and invasion of SW480 cells as that of the overexpression of miR‑145. Furthermore, it was observed that the inhibition of miR‑145 did not reverse the effects of MAPK1 silencing on SW480 cells. However, the overexpression of MAPK1 led to considerable reversal of the effects of the overexpression of miR‑145 on the proliferation, migration and invasion of SW480 cells. The effects of the overexpression of miR‑145 were also evaluated in vivo in xenografted mice and it was observed that the overexpression of miR‑145 also inhibited tumor growth and volume in vivo. Taken together, it was concluded that miR‑145 may prove to be an important therapeutic target for colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Li XJ, Li P and Guo XT: MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1. Int J Mol Med 42: 3171-3180, 2018.
APA
Yang, Y., Li, X., Li, P., & Guo, X. (2018). MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1. International Journal of Molecular Medicine, 42, 3171-3180. https://doi.org/10.3892/ijmm.2018.3904
MLA
Yang, Y., Li, X., Li, P., Guo, X."MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1". International Journal of Molecular Medicine 42.6 (2018): 3171-3180.
Chicago
Yang, Y., Li, X., Li, P., Guo, X."MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1". International Journal of Molecular Medicine 42, no. 6 (2018): 3171-3180. https://doi.org/10.3892/ijmm.2018.3904
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Li XJ, Li P and Guo XT: MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1. Int J Mol Med 42: 3171-3180, 2018.
APA
Yang, Y., Li, X., Li, P., & Guo, X. (2018). MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1. International Journal of Molecular Medicine, 42, 3171-3180. https://doi.org/10.3892/ijmm.2018.3904
MLA
Yang, Y., Li, X., Li, P., Guo, X."MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1". International Journal of Molecular Medicine 42.6 (2018): 3171-3180.
Chicago
Yang, Y., Li, X., Li, P., Guo, X."MicroRNA-145 regulates the proliferation, migration and invasion of human primary colon adenocarcinoma cells by targeting MAPK1". International Journal of Molecular Medicine 42, no. 6 (2018): 3171-3180. https://doi.org/10.3892/ijmm.2018.3904
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