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Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis

  • Authors:
    • Tianzhu Lu
    • Hongjing Liu
    • Ganhua You
  • View Affiliations / Copyright

    Affiliations: Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, General Department, Stomatological Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Medical College, Guizhou University, Guiyang, Guizhou 550025, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3291-3299
    |
    Published online on: October 2, 2018
       https://doi.org/10.3892/ijmm.2018.3913
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Abstract

Oral squamous cell carcinoma (OSCC) is a major cancer type in the head and neck region. Recent studies have reported a marked rise in the incidence of OSCC. The present study was performed to better understand the roles that long non‑coding RNAs (lncRNAs) serve in OSCC carcinogenesis. The levels of the lncRNA C5orf66 antisense RNA 1 (C5orf66‑AS1) and of cytochrome c1 (CYC1) in OSCC tissues and cells were measured through reverse transcription‑quantitative polymerase chain reaction. In addition, the levels of associated proteins were analyzed by western blotting, while MTT assay was used to detect the cell proliferation ability. Wound healing and transwell assays were also used to detect the migration and invasion abilities of OSCC cells in the experimental groups, while flow cytometry was applied to analyze cell apoptosis. The findings revealed that the expression of lncRNA C5orf66‑AS1 in OSCC tissues and cells was significantly decreased. Overexpression of lncRNA C5orf66‑AS1 significantly inhibited the proliferation, invasion and migration ability of OSCC cells, and promoted cell apoptosis, while lncRNA C5orf66‑AS1 downregulation presented the opposite effects. In addition, it was observed that CYC1 was upregulated in OSCC tissues and cells, and was negatively regulated by lncRNA C5orf66‑AS1. Notably, CYC1 silencing markedly eliminated the effects of lncRNA C5orf66‑AS1 downregulation on OSCC cells. Taken together, these findings indicated that lncRNA C5orf66‑AS1 may prevent OSCC progression by inhibiting OSCC cell growth and metastasis via the regulation of CYC1 expression.
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1 

Ryerson AB, Eheman CR, Altekruse SF, Ward JW, Jemal A, Sherman RL, Henley SJ, Holtzman D, Lake A, Noone AM, et al: Annual report to the nation on the status of cancer, 1975–2012 featuring the increasing incidence of liver cancer. Cancer. 122:1312–1337. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar

3 

Gershenwald JE, Soong SJ and Balch CM; American Joint Committee on Cancer (AJCC) Melanoma Staging Committee: 2010.TNM staging system for cutaneous melanoma…and beyond. Ann Surg Oncol. 17:1475–1477. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Kreppel M, Drebber U, Rothamel D, Eich HT, Kübler A, Scheer M and Zöller JE: Prognostic impact of different TNM-based stage groupings for oral squamous cell carcinoma. Head Neck. 33:1467–1475. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Kolk A, Jubitz N, Mengele K, Mantwill K, Bissinger O, Schmitt M, Kremer M and Holm PS: Expression of Y-box-binding protein YB-1 allows stratification into long- and short-term survivors of head and neck cancer patients. Br J Cancer. 105:1864–1873. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Götz C, Drecoll E, Straub M, Bissinger O, Wolff KD and Kolk A: Impact of HPV infection on oral squamous cell carcinoma. Oncotarget. 7:76704–76712. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Scully C and Bagan J: Oral squamous cell carcinoma overview. Oral Oncol. 45:301–308. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Davidovich C and Cech TR: The recruitment of chromatin modifiers by long noncoding RNAs: Lessons from PRC2. RNA. 21:2007–2022. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Rogers SN, Brown JS, Woolgar JA, Lowe D, Magennis P, Shaw RJ, Sutton D, Errington D and Vaughan D: Survival following primary surgery for oral cancer. Oral Oncol. 45:201–211. 2009. View Article : Google Scholar

10 

Nagtegaal ID, Quirke P and Schmoll HJ: Has the new TNM classification for colorectal cancer improved care. Nat Rev Clin Oncol. 9:119–123. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Piazzolla D, Palla AR, Pantoja C, Cañamero M, de Castro IP, Ortega S, Gómez-López G, Dominguez O, Megías D, Roncador G, et al: Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia. Nat Commun. 5:42262014. View Article : Google Scholar : PubMed/NCBI

12 

Balch C and Nephew KP: The role of chromatin, microRNAs, and tumor stem cells in ovarian cancer. Cancer Biomark. 8:203–221. 2010–2011. PubMed/NCBI

13 

Sun T: Long noncoding RNAs act as regulators of autophagy in cancer. Pharmacol Res. 129:151–155. 2018. View Article : Google Scholar

14 

Carvalho AL, Nishimoto IN, Califano JA and Kowalski LP: Trends in incidence and prognosis for head and neck cancer in the United States: A site-specific analysis of the SEER database. Int J Cancer. 114:806–816. 2005. View Article : Google Scholar

15 

Gutschner T and Diederichs S: The hallmarks of cancer: A long non-coding RNA point of view. RNA Biol. 9:703–719. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Min A, Zhu C, Peng S, Rajthala S, Costea DE and Sapkota D: MicroRNAs as important players and biomarkers in oral carcinogenesis. Biomed Res Int. 2015.186904:2015.

17 

Feng L, Houck JR, Lohavanichbutr P and Chen C: Transcriptome analysis reveals differentially expressed lncRNAs between oral squamous cellcarcinoma and healthy oral mucosa. Oncotarget. 8:31521–31531. 2017.PubMed/NCBI

18 

Maruyama R and Suzuki H: Long noncoding RNA involvement in cancer. BMB Rep. 45:604–611. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Quinn JJ and Chang HY: Unique features of long non-coding RNA biogenesis and function. Nat Rev Genet. 17:47–62. 2016. View Article : Google Scholar

20 

Bartonicek N, Maag JL and Dinger ME: Long noncoding RNAs in cancer: Mechanisms of action and technological advancements. Mol Cancer. 15:432016. View Article : Google Scholar : PubMed/NCBI

21 

Yang Q, Xu E, Dai J, Liu B, Han Z, Wu J, Zhang S, Peng B, Zhang Y and Jiang Y: A novel long noncoding RNA AK001796 acts as an oncogene and is involved in cell growth inhibition by resveratrol in lung cancer. Toxicol Appl Pharmacol. 285:79–88. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A and Lawrence JB: An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles. Mol Cell. 33:717–726. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Nie W, Ge HJ, Yang XQ, Sun X, Huang H, Tao X, Chen WS and Li B: LncRNA-UCA1 exerts oncogenic functions in non-small cell lung cancer by targeting miR-193a-3p. Cancer Lett. 371:99–106. 2016. View Article : Google Scholar

24 

Peng W, Si S, Zhang Q, Li C, Zhao F, Wang F, Yu J and Ma R: Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression. J Exp Clin Cancer Res. 34:792015. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

26 

SHahinas J and Hysi D: Methods and risk of bias in molecular marker prognosis studies in oral squamous cell carcinoma. Oral Dis. 24:115–119. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Peurala E, Tuominen M, Löyttyniemi E, Syrjänen S and Rautava J: Eosinophilia is a favorable prognostic marker for oral cavity and lip squamous cell carcinoma. APMIS. 126:201–207. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Lee EY and Muller WJ: Oncogenes and tumor suppressor genes. Cold Spring Harb Perspect Biol. 2:a0032362010. View Article : Google Scholar : PubMed/NCBI

29 

Iurlaro R, León-Annicchiarico CL and Muñoz-Pinedo C: Regulation of cancer metabolism by oncogenes and tumor suppressors. Methods Enzymol. 542:59–80. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Wang D, Qiu C, Zhang H, Wang J, Cui Q and Yin Y: Human microRNA oncogenes and tumor suppressors show significantly different biological patterns: From functions to targets. PLoS One. 5:e130672010. View Article : Google Scholar : PubMed/NCBI

31 

Geisler S and Coller J: RNA in unexpected places: Long non-coding RNA functions in diverse cellular contexts. Nat Rev Mol Cell Biol. 14:699–712. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Han Y, Sun S, Zhao M, Zhang Z, Gong S, Gao P, Liu J, Zhou J, Ma D, Gao Q and Wu P: CYC1 predicts poor prognosis in patients with breast cancer. Dis Markers. 2016.3528064:2016.

33 

Li G, Fu D, Liang W, Fan L, Chen K, Shan L, Hu S, Ma X, Zhou K and Cheng B: CYC1 silencing sensitizes osteosarcoma cells to TRAIL-induced apoptosis. Cell Physiol Biochem. 34:2070–2080. 2014. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lu T, Liu H and You G: Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis. Int J Mol Med 42: 3291-3299, 2018.
APA
Lu, T., Liu, H., & You, G. (2018). Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis. International Journal of Molecular Medicine, 42, 3291-3299. https://doi.org/10.3892/ijmm.2018.3913
MLA
Lu, T., Liu, H., You, G."Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis". International Journal of Molecular Medicine 42.6 (2018): 3291-3299.
Chicago
Lu, T., Liu, H., You, G."Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis". International Journal of Molecular Medicine 42, no. 6 (2018): 3291-3299. https://doi.org/10.3892/ijmm.2018.3913
Copy and paste a formatted citation
x
Spandidos Publications style
Lu T, Liu H and You G: Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis. Int J Mol Med 42: 3291-3299, 2018.
APA
Lu, T., Liu, H., & You, G. (2018). Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis. International Journal of Molecular Medicine, 42, 3291-3299. https://doi.org/10.3892/ijmm.2018.3913
MLA
Lu, T., Liu, H., You, G."Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis". International Journal of Molecular Medicine 42.6 (2018): 3291-3299.
Chicago
Lu, T., Liu, H., You, G."Long non-coding RNA C5orf66-AS1 prevents oral squamous cell carcinoma through inhibiting cell growth and metastasis". International Journal of Molecular Medicine 42, no. 6 (2018): 3291-3299. https://doi.org/10.3892/ijmm.2018.3913
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