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Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways

  • Authors:
    • Fuguo Chen
    • Dan Bi
    • Chen Cheng
    • Sunxiang Ma
    • Yang Liu
    • Kaixiang Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 37-46
    |
    Published online on: October 17, 2018
       https://doi.org/10.3892/ijmm.2018.3938
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Abstract

The skin, as the largest organ of the human body, is an important source of stromal stem cells with multipotent differentiation potential. CD105+ mesenchymal stem cells exhibit a higher level of stemness than CD105‑ cells. In the present study, human dermal‑derived CD105+ fibroblast cells (CD105+ hDDFCs) were isolated from human foreskin specimens using immunomagnetic isolation methods to examine the role of bone morphogenetic protein (BMP)‑7 in osteogenic differentiation. Adenovirus‑mediated recombinant BMP7 expression enhanced osteogenesis‑associated gene expression, calcium deposition, and alkaline phosphatase activity. Investigation of the underlying mechanisms showed that BMP7 activated small mothers against decapentaplegic (Smad) and p38/mitogen‑activated protein kinase signaling in CD105+ hDDFCs. The small interfering RNA‑mediated knockdown of Smad4 or inhibition of p38 attenuated the BMP7‑induced enhancement of osteogenic differentiation. In an in vivo ectopic bone formation model, the adenovirus‑mediated overexpression of BMP7 enhanced bone formation from CD105+ hDDFCs. Taken together, these data indicated that adenoviral BMP7 gene transfer in CD105+ hDDFCs may be developed as an effective tool for bone tissue engineering.
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1 

Sorrell JM and Caplan AI: Fibroblasts-a diverse population at the center of it all. Int Rev Cell Mol Biol. 276:161–214. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Fang F, Ni K, Cai Y, Ye Z, Shang J, Shen S and Xiong C: Biological characters of human dermal fibroblasts derived from foreskin of male infertile patients. Tissue Cell. 49:56–63. 2017. View Article : Google Scholar

3 

Bussmann BM, Reiche S, Marí-Buyé N, Castells-Sala C, Meisel HJ and Semino CE: Chondrogenic potential of human dermal fibroblasts in a contractile, soft, self-assembling, peptide hydrogel. J Tissue Eng Regen Med. 10:E54–E62. 2016. View Article : Google Scholar

4 

Guerreiro SG, Oliveira MJ, Barbosa MA, Soares R and Granja PL: Neonatal human dermal fibroblasts immobilized in RGD-alginate induce angiogenesis. Cell Transplant. 23:945–957. 2014. View Article : Google Scholar

5 

Lee SB, Shim S, Kim MJ, Shin HY, Jang WS, Lee SJ, Jin YW, Lee SS and Park S: Identification of a distinct subpopulation of fibroblasts from murine dermis: CD73(-) CD105(+) as potential marker of dermal fibroblasts subset with multipotency. Cell Biol Int. 40:1008–1016. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj and Horwitz E: Minimal criteria for defining multipotent mesen-chymal stromal cells. The International Society for cellular therapy position statement. Cytotherapy. 8:315–317. 2006. View Article : Google Scholar

7 

Lorenz K, Sicker M, Schmelzer E, Rupf T, Salvetter J, Schulz-Siegmund M and Bader A: Multilineage differentiation potential of human dermal skin-derived fibroblasts. Exp Dermatol. 17:925–932. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Miyazono K, Maeda S and Imamura T: BMP receptor signaling: Transcriptional targets, regulation of signals, and signaling cross-talk. Cytokine Growth Factor Rev. 16:251–263. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Chen G, Deng C and Li YP: TGF-β and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci. 8:272–288. 2012. View Article : Google Scholar :

10 

Lee KS, Hong SH and Bae SC: Both the Smad and p38 MAPK pathways play a crucial role in Runx2 expression following induction by transforming growth factor-beta and bone morphogenetic protein. Oncogene. 21:7156–7163. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Zhang X, Guo J, Wu G and Zhou Y: Effects of heterodimeric bone morphogenetic protein-2/7 on osteogenesis of human adipose-derived stem cells. Cell Prolif. 48:650–660. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Myllylä RM, Haapasaari KM, Lehenkari P and Tuukkanen J: Bone morphogenetic proteins 4 and 2/7 induce osteogenic differentiation of mouse skin derived fibroblast and dermal papilla cells. Cell Tissue Res. 355:463–470. 2014. View Article : Google Scholar

13 

Chen FG, Zhang WJ, Bi D, Liu W, Wei X, Chen FF, Zhu L, Cui L and Cao Y: Clonal analysis of nestin(−) vimentin(+) multi-potent fibroblasts isolated from human dermis. J Cell Sci. 120:2875–2883. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Aslan H, Zilberman Y, Kandel L, Liebergall M, Oskouian RJ, Gazit D and Gazit Z: Osteogenic differentiation of noncultured immunoisolated bone marrow-derived CD105+ cells. Stem Cells. 24:1728–1737. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

16 

Crigler L, Kazhanie A, Yoon TJ, Zakhari J, Anders J, Taylor B and Virador VM: Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages. FASEB J. 21:2050–2063. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Toma JG, Akhavan M, Fernandes KJ, Barnabé-Heider F, Sadikot A, Kaplan DR and Miller FD: Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol. 3:778–784. 2001. View Article : Google Scholar : PubMed/NCBI

18 

French MM, Rose S, Canseco J and Athanasiou KA: Chondrogenic differentiation of adult dermal fibroblasts. Ann Biomed Eng. 32:50–56. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Hirata K, Tsukazaki T, Kadowaki A, Furukawa K, Shibata Y, Moriishi T, Okubo Y, Bessho K, Komori T, Mizuno A and Yamaguchi A: Transplantation of skin fibroblasts expressing BMP-2 promotes bone repair more effectively than those expressing Runx2. Bone. 32:502–512. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Brendel C, Kuklick L, Hartmann O, Kim TD, Boudriot U, Schwell D and Neubauer A: Distinct gene expression profile of human mesenchymal stem cells in comparison to skin fibroblasts employing cDNA microarray analysis of 9600 genes. Gene Expr. 12:245–257. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Jeney F, Bazsó-Dombi E, Oravecz K, Szabó J and Nagy IZ: Cytochemical studies on the fibroblast-preadipocyte relationships in cultured fibroblast cell lines. Acta Histochem. 102:381–389. 2000. View Article : Google Scholar

22 

Rutherford RB, Moalli M, Franceschi RT, Wang D, Gu K and Krebsbach PH: Bone morphogenetic protein-transduced human fibroblasts convert to osteoblasts and form bone in vivo. Tissue Eng. 8:441–452. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Mizuno S and Glowacki J: Low oxygen tension enhances chondroinduction by demineralized bone matrix in human dermal fibroblasts in vitro. Cells Tissues Organs. 180:151–158. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Lysy PA, Smets F, Sibille C, Najimi M and Sokal EM: Human skin fibroblasts: From mesodermal to hepatocyte-like differentiation. Hepatology. 46:1574–1585. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Krebsbach PH, Gu K, Franceschi RT and Rutherford RB: Gene therapy-directed osteogenesis: BMP-7-transduced human fibroblasts form bone in vivo. Hum Gene Ther. 11:1201–1210. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Mao CY, Wang YG, Zhang X, Zheng XY, Tang TT and Lu EY: Double-edged-sword effect of IL-1beta on the osteogenesis of periodontal ligament stem cells via crosstalk between the NF-kappaB, MAPK and BMP/Smad signaling pathways. Cell Death Dis. 7:e22962016. View Article : Google Scholar

27 

Moustakas A, Souchelnytskyi S and Heldin CH: Smad regulation in TGF-beta signal transduction. J Cell Sci. 114:4359–4369. 2001.

28 

Guo X and Wang XF: Signaling cross-talk between TGF-beta/BMP and other pathways. Cell Res. 19:71–88. 2009. View Article : Google Scholar

29 

Beederman M, Lamplot JD, Nan G, Wang J, Liu X, Yin L, Li R, Shui W, Zhang H, Kim SH, et al: BMP signaling in mesenchymal stem cell differentiation and bone formation. J Biomed Sci Eng. 6:32–52. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Choi H, Jeong BC, Kook MS and Koh JT: Betulinic acid synergically enhances BMP2-induced bone formation via stimulating Smad 1/5/8 and p38 pathways. J Biomed Sci. 23:452016. View Article : Google Scholar : PubMed/NCBI

31 

Horikiri Y, Shimo T, Kurio N, Okui T, Matsumoto K, Iwamoto M and Sasaki A: Sonic hedgehog regulates osteoblast function by focal adhesion kinase signaling in the process of fracture healing. PLoS One. 8:e767852013. View Article : Google Scholar : PubMed/NCBI

32 

Reichert JC, Schmalzl J, Prager P, Gilbert F, Quent VM, Steinert AF, Rudert M and Nöth U: Synergistic effect of Indian hedgehog and bone morphogenetic protein-2 gene transfer to increase the osteogenic potential of human mesenchymal stem cells. Stem Cell Res Ther. 4:1052013. View Article : Google Scholar : PubMed/NCBI

33 

Kim JH, Liu X, Wang J, Chen X, Zhang H, Kim SH, Cui J, Li R, Zhang W, Kong Y, et al: Wnt signaling in bone formation and its therapeutic potential for bone diseases. Ther Adv Musculoskelet Dis. 5:13–31. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Bessa PC, Casal M and Reis RL: Bone morphogenetic proteins in tissue engineering: The road from the laboratory to the clinic, part I (basic concepts). J Tissue Eng Regen Med. 2:1–13. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Rahman MS, Akhtar N, Jamil HM, Banik RS and Asaduzzaman SM: TGF-β/BMP signaling and other molecular events: Regulation of osteoblastogenesis and bone formation. Bone Res. 3:150052015. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Chen F, Bi D, Cheng C, Ma S, Liu Y and Cheng K: Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways . Int J Mol Med 43: 37-46, 2019.
APA
Chen, F., Bi, D., Cheng, C., Ma, S., Liu, Y., & Cheng, K. (2019). Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways . International Journal of Molecular Medicine, 43, 37-46. https://doi.org/10.3892/ijmm.2018.3938
MLA
Chen, F., Bi, D., Cheng, C., Ma, S., Liu, Y., Cheng, K."Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways ". International Journal of Molecular Medicine 43.1 (2019): 37-46.
Chicago
Chen, F., Bi, D., Cheng, C., Ma, S., Liu, Y., Cheng, K."Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways ". International Journal of Molecular Medicine 43, no. 1 (2019): 37-46. https://doi.org/10.3892/ijmm.2018.3938
Copy and paste a formatted citation
x
Spandidos Publications style
Chen F, Bi D, Cheng C, Ma S, Liu Y and Cheng K: Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways . Int J Mol Med 43: 37-46, 2019.
APA
Chen, F., Bi, D., Cheng, C., Ma, S., Liu, Y., & Cheng, K. (2019). Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways . International Journal of Molecular Medicine, 43, 37-46. https://doi.org/10.3892/ijmm.2018.3938
MLA
Chen, F., Bi, D., Cheng, C., Ma, S., Liu, Y., Cheng, K."Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways ". International Journal of Molecular Medicine 43.1 (2019): 37-46.
Chicago
Chen, F., Bi, D., Cheng, C., Ma, S., Liu, Y., Cheng, K."Bone morphogenetic protein 7 enhances the osteogenic differentiation of human dermal-derived CD105+ fibroblast cells through the Smad and MAPK pathways ". International Journal of Molecular Medicine 43, no. 1 (2019): 37-46. https://doi.org/10.3892/ijmm.2018.3938
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