Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis

Ci,Chao; Tang,Biao; Lyu,Dalun; Liu,Wenbei; Qiang,Di; Ji,Xiang; Qiu,Xiamin; Chen,Lei; Ding,Wei

doi:10.3892/ijmm.2018.3985

Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis

Authors:
- Chao Ci
- Biao Tang
- Dalun Lyu
- Wenbei Liu
- Di Qiang
- Xiang Ji
- Xiamin Qiu
- Lei Chen
- Wei Ding
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Affiliations: Department of Dermatology, First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Burn and Plastic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China
Published online on: November 7, 2018 https://doi.org/10.3892/ijmm.2018.3985
Pages: 404-412
Copyright: © Ci et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cutaneous melanoma is very aggressive and results in high mortality rates for cancer patients. Determining molecular targets is important for developing novel therapies for cutaneous melanoma. Cell division cycle associated 8 (CDCA8) is a putative oncogene that is upregulated in multiple types of cancer. The present study aimed to examine the role of CDCA8 in cutaneous melanoma, with a focus on the association of its expression to prognosis and metastasis. First, the mRNA expression of CDCA8 in cutaneous melanoma tissues was investigated using the ONCOMINE and Gene Expression Omnibus (GEO) databases. Furthermore, the relationship between the expression of CDCA8 and cutaneous melanoma patient survival was analyzed using a Kaplan‑Meier plot and Log Rank test. In addition, the effects of CDCA8 on proliferation, migration and invasion of cutaneous melanoma cell lines were investigated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), Cell Counting kit‑8, colony formation assay, wound healing and Matrigel assay. Finally, the expression levels of key proteins related to the Rho‑associated coiled‑coil‑containing protein kinase (ROCK) signaling pathway were measured by western blot assay. The results demonstrated that CDCA8 was overexpressed in cutaneous melanoma tissues and cells lines compared with normal tissues, and high expression of CDCA8 was significantly associated with poorer prognosis in patients with cutaneous melanoma. In in vitro experiments, CDCA8 knockdown inhibited A375 and MV3 cell proliferation, migration and invasion. In addition, CDCA8 knockdown reduced the phosphorylation levels of ROCK1 and myosin light chain, two downstream effector proteins of the ROCK pathway. In summary, the present findings suggested that CDCA8 may be a promising therapeutic target for the treatment of cutaneous melanoma.

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