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Article

Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis

  • Authors:
    • Yuedong Zhang
    • Hongwei Zhang
    • Huiqing Kang
    • Wenjie Huo
    • Yu Zhou
    • Yuchao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Wuhai People's Hospital, Wuhai, Inner Mongolia Autonomous Region 016000, P.R. China, Department of Pathology, Wuhai People's Hospital, Wuhai, Inner Mongolia Autonomous Region 016000, P.R. China
  • Pages: 1049-1057
    |
    Published online on: November 26, 2018
       https://doi.org/10.3892/ijmm.2018.3995
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Abstract

The upregulation of long non‑coding RNA (lncRNA) human ovarian cancer‑specific transcript 2 (HOST2) has been identified in breast cancer. The present study aimed to investigate whether lncRNA HOST2 regulated the proliferation of triple negative breast cancer (TNBC) cells, and the underlying molecular mechanism. In total, 30 patients with primary TNBC, who were treated at Wuhai People's Hospital (Wuhai, China), were recruited for the present study. Reverse transcription‑quantitative polymerase chain reaction analysis was used for the examination of gene expression levels. A Cell Counting kit‑8 (CCK‑8) assay was used for the detection of cell proliferation. Phases of the cell cycle were evaluated by flow cytometry. Western blot analysis was performed to detect protein expression levels. A dual luciferase activity assay was performed to examine the interaction between microRNA (miRNA) and the 3' untranslated region (UTR) of target mRNA. The results revealed increased expression levels of HOST2 in tumor tissues from patients with TNBC. A positive correlation was identified between the expression of HOST2 and cyclin‑dependent kinase 6 (CDK6) in tumor tissues. The silencing of HOST2 induced cell proliferation inhibition and cell cycle redistribution in MDA‑MB‑231 and MDA‑MB‑468 TNBC cells. In these two cell lines, HOST2 silencing caused a decrease in the phosphorylation of RB1 and CDK6, which was observed at the mRNA and protein levels. However, the silencing of CDK6 did not alter the expression of HOST2. It was hypothesized and confirmed that let‑7b, a previously reported target miRNA of HOST2, was able to directly bind to the 3'UTR of CDK6 and repress its expression. The expression of let‑7b was negatively correlated with the expression of HOST2 and CDK6 in tumor tissues. Overall, the data suggested that lncRNA HOST2 acts as an oncogene in TNBC via the upregulation of CDK6.
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1 

Schettini F, Buono G, Cardalesi C, Desideri I, De Placido S and Del Mastro L: Hormone receptor/human epidermal growth factor receptor 2-positive breast cancer: Where we are now and where we are going. Cancer Treat Rev. 46:20–26. 2016. View Article : Google Scholar

2 

Zheng S, Bai JQ, Li J, Fan JH, Pang Y, Song QK, Huang R, Yang HJ, Xu F, Lu N and Qiao YL: The pathologic characteristics of breast cancer in China and its shift during 1999-2008: a national-wide multicenter cross-sectional image over 10 years. Int J Cancer. 131:2622–2631. 2012. View Article : Google Scholar

3 

Fan L, Strasser-Weippl K, Li JJ, St Louis J, Finkelstein DM, Yu KD, Chen WQ, Shao ZM and Goss P: Breast cancer in China. Lancet Oncol. 15:e279–e289. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Comprehensive molecular portraits of human breast tumours. Nature. 490:61–70. 2012. View Article : Google Scholar

5 

Agrawal LS and Mayer IA: Platinum agents in the treatment of early-stage triple-negative breast cancer: Is it time to change practice? Clin Adv Hematol Oncol. 12:654–658. 2014.

6 

Foulkes WD, Smith IE and Reis-Filho JS: Triple-negative breast cancer. N Engl J Med. 363:1938–1948. 2010. View Article : Google Scholar

7 

Pinto R, De Summa S, Pilato B and Tommasi S: DNA methylation and miRNAs regulation in hereditary breast cancer: Epigenetic changes, players in transcriptional and post-transcriptional regulation in hereditary breast cancer. Curr Mol Med. 14:45–57. 2014. View Article : Google Scholar

8 

Maruyama R and Suzuki H: Long noncoding RNA involvement in cancer. BMB Rep. 45:604–611. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Li JP, Liu LH, Li J, Chen Y, Jiang XW, Ouyang YR, Liu YQ, Zhong H, Li H and Xiao T: Microarray expression profile of long noncoding RNAs in human osteosarcoma. Biochem Biophys Res Commun. 433:200–206. 2013. View Article : Google Scholar

10 

Lv M, Xu P, Wu Y, Huang L, Li W, Lv S, Wu X, Zeng X, Shen R, Jia X, et al: LncRNAs as new biomarkers to differentiate triple negative breast cancer from non-triple negative breast cancer. Oncotarget. 7:13047–13059. 2016. View Article : Google Scholar

11 

Rinn JL and Chang HY: Genome regulation by long noncoding RNAs. Annu Rev Biochem. 81:145–166. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Liu RT, Cao JL, Yan CQ, Wang Y, An CJ and Lv HT: Effects of LncRNA-HOST2 on cell proliferation, migration, invasion and apoptosis of human hepatocellular carcinoma cell line SMMC-7721. Biosci Rep. 37:2017. View Article : Google Scholar

13 

Zhong Y, Gao D, He S, Shuai C and Peng S: Dysregulated expression of long noncoding RNAs in ovarian cancer. Int J Gynecol Cancer. 26:1564–1570. 2016. View Article : Google Scholar

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar

15 

Malumbres M and Barbacid M: Mammalian cyclin-dependent kinases. Trends Biochem Sci. 30:630–641. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Lim S and Kaldis P: Cdks, cyclins and CKIs: roles beyond cell cycle regulation. Development. 140:3079–3093. 2013. View Article : Google Scholar

17 

Qi X, Zhang DH, Wu N, Xiao JH, Wang X and Ma W: ceRNA in cancer: Possible functions and clinical implications. J Med Genet. 52:710–718. 2015. View Article : Google Scholar

18 

Lu PW, Li L, Wang F and Gu YT: Effects of long non-coding RNA HOST2 on cell migration and invasion by regulating microRNA let-7b in breast cancer. J Cell Biochem. 119:4570–4580. 2017. View Article : Google Scholar

19 

Palma G, Frasci G, Chirico A, Esposito E, Siani C, Saturnino C, Arra C, Ciliberto G, Giordano A and D'Aiuto M: Triple negative breast cancer: Looking for the missing link between biology and treatments. Oncotarget. 6:26560–26574. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Oakman C, Viale G and Di Leo A: Management of triple negative breast cancer. Breast. 19:312–321. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Zhang Y, He Q, Hu Z, Feng Y, Fan L, Tang Z, Yuan J, Shan W, Li C, Hu X, et al: Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer. Nat Struct Mol Biol. 23:522–530. 2016. View Article : Google Scholar :

22 

Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, Wang C, Hawke DH, Wang S, Zhang Y, et al: The LINK-A lncRNA activates normoxic HIF1alpha signalling in triple-negative breast cancer. Nat Cell Biol. 18:213–224. 2016. View Article : Google Scholar

23 

Pickard MR and Williams GT: Regulation of apoptosis by long non-coding RNA GAS5 in breast cancer cells: implications for chemotherapy. Breast Cancer Res Treat. 145:359–370. 2014. View Article : Google Scholar

24 

Rangel LB, Sherman-Baust CA, Wernyj RP, Schwartz DR, Cho KR and Morin PJ: Characterization of novel human ovarian cancer-specific transcripts (HOSTs) identified by serial analysis of gene expression. Oncogene. 22:7225–7232. 2003. View Article : Google Scholar

25 

Gao Y, Meng H, Liu S, Hu J, Zhang Y, Jiao T, Liu Y, Ou J, Wang D, Yao L, et al: LncRNA-HOST2 regulates cell biological behaviors in epithelial ovarian cancer through a mechanism involving microRNA let-7b. Hum Mol Genet. 24:841–852. 2015. View Article : Google Scholar

26 

Wang W, Li X, Meng FB, Wang ZX, Zhao RT and Yang CY: Effects of the long non-coding RNA HOST2 on the proliferation, migration, invasion and apoptosis of human osteosarcoma cells. Cell Physiol Biochem. 43:320–330. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Tadesse S, Yu M, Kumarasiri M, Le BT and Wang S: Targeting CDK6 in cancer: State of the art and new insights. Cell Cycle. 14:3220–3230. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Hsu YH, Yao J, Chan LC, Wu TJ, Hsu JL, Fang YF, Wei Y, Wu Y, Huang WC, Liu CL, et al: Definition of PKC-alpha, CDK6, and MET as therapeutic targets in triple-negative breast cancer. Cancer Res. 74:4822–4835. 2014. View Article : Google Scholar

29 

Liu X, Li D, Zhang W, Guo M and Zhan Q: Long non-coding RNA gadd7 interacts with TDP-43 and regulates Cdk6 mRNA decay. EMBO J. 31:4415–4427. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Liu Z, Wang W, Jiang J, Bao E, Xu D, Zeng Y, Tao L and Qiu J: Downregulation of GAS5 promotes bladder cancer cell proliferation, partly by regulating CDK6. PLoS One. 8:e739912013. View Article : Google Scholar

31 

Kawasaki Y, Komiya M, Matsumura K, Negishi L, Suda S, Okuno M, Yokota N, Osada T, Nagashima T, Hiyoshi M, et al: MYU, a target lncRNA for Wnt/c-Myc signaling, mediates induction of CDK6 to promote cell cycle progression. Cell Rep. 16:2554–2564. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J: Natural RNA circles function as efficient microRNA sponges. Nature. 495:384–388. 2013. View Article : Google Scholar

33 

Salmena L, Poliseno L, Tay Y, Kats L and Pandolfi PP: A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? Cell. 146:353–358. 2011. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zhang H, Kang H, Huo W, Zhou Y and Zhang Y: Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis. Int J Mol Med 43: 1049-1057, 2019.
APA
Zhang, Y., Zhang, H., Kang, H., Huo, W., Zhou, Y., & Zhang, Y. (2019). Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis. International Journal of Molecular Medicine, 43, 1049-1057. https://doi.org/10.3892/ijmm.2018.3995
MLA
Zhang, Y., Zhang, H., Kang, H., Huo, W., Zhou, Y., Zhang, Y."Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis". International Journal of Molecular Medicine 43.2 (2019): 1049-1057.
Chicago
Zhang, Y., Zhang, H., Kang, H., Huo, W., Zhou, Y., Zhang, Y."Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis". International Journal of Molecular Medicine 43, no. 2 (2019): 1049-1057. https://doi.org/10.3892/ijmm.2018.3995
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zhang H, Kang H, Huo W, Zhou Y and Zhang Y: Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis. Int J Mol Med 43: 1049-1057, 2019.
APA
Zhang, Y., Zhang, H., Kang, H., Huo, W., Zhou, Y., & Zhang, Y. (2019). Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis. International Journal of Molecular Medicine, 43, 1049-1057. https://doi.org/10.3892/ijmm.2018.3995
MLA
Zhang, Y., Zhang, H., Kang, H., Huo, W., Zhou, Y., Zhang, Y."Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis". International Journal of Molecular Medicine 43.2 (2019): 1049-1057.
Chicago
Zhang, Y., Zhang, H., Kang, H., Huo, W., Zhou, Y., Zhang, Y."Knockdown of long non-coding RNA HOST2 inhibits the proliferation of triple negative breast cancer via regulation of the let-7b/CDK6 axis". International Journal of Molecular Medicine 43, no. 2 (2019): 1049-1057. https://doi.org/10.3892/ijmm.2018.3995
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