Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro

  • Authors:
    • Bo Qiu
    • Xiong‑Feng Xu
    • Rong‑Hui Deng
    • Gan‑Qing Xia
    • Xi‑Fu Shang
    • Pang‑Hu Zhou
  • View Affiliations

  • Published online on: November 26, 2018     https://doi.org/10.3892/ijmm.2018.3997
  • Pages: 1076-1084
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Abstract

Osteoarthritis (OA) is a common degenerative joint disease characterized by inflammation of synoviocytes and degradation of cartilage. In the present study, hyaluronic acid/chitosan (HA/CS) nanoparticles were used as a vehicle for gene therapy of OA, and the cytokine response modifier A (CrmA) pDNA was proposed as the target gene. The HA/CS/pCrmA nanoparticles were prepared and the characteristics of the nanoparticles were examined. The nanoparticles were spherical, and the smallest size was obtained with the HA:CS weight ratio of 1:4. The release analysis exhibited a constant release over 29 days. The pDNA was completely combined with HA/CS nanoparticles and the HA/CS nanoparticles protected pDNA from degradation. Subsequently, rat synoviocytes were transfected with HA/CS/pDNA nanoparticles, and the results demonstrated that the HA/CS nanoparticles were able to improve the transfection capacity of pDNA. The cytotoxicity of the HA/CS/pDNA nanoparticles was additionally detected using a MTS assay to ensure that the HA/CS nanoparticle was a safe carrier. To additionally investigate the effects of HA/CS/pCrmA nanoparticles on synoviocytes in OA, the MMP‑3 and MMP‑13 gene expression levels were detected at the gene and protein expression levels. These results indicated that the HA/CS/pCrmA nanoparticles attenuated interleukin‑1β‑mediated inflammation in synoviocytes. It was concluded that the HA/CS/pCrmA nanoparticles may provide a novel approach to the treatment of OA.
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February-2019
Volume 43 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Qiu B, Xu XF, Deng RH, Xia GQ, Shang XF and Zhou PH: Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro. Int J Mol Med 43: 1076-1084, 2019
APA
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., & Zhou, P. (2019). Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro. International Journal of Molecular Medicine, 43, 1076-1084. https://doi.org/10.3892/ijmm.2018.3997
MLA
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., Zhou, P."Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro". International Journal of Molecular Medicine 43.2 (2019): 1076-1084.
Chicago
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., Zhou, P."Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro". International Journal of Molecular Medicine 43, no. 2 (2019): 1076-1084. https://doi.org/10.3892/ijmm.2018.3997