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Article

Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro

  • Authors:
    • Bo Qiu
    • Xiong‑Feng Xu
    • Rong‑Hui Deng
    • Gan‑Qing Xia
    • Xi‑Fu Shang
    • Pang‑Hu Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Orthopedics, Anhui Provincial Hospital, Hefei, Anhui 230000, P.R. China
  • Pages: 1076-1084
    |
    Published online on: November 26, 2018
       https://doi.org/10.3892/ijmm.2018.3997
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Abstract

Osteoarthritis (OA) is a common degenerative joint disease characterized by inflammation of synoviocytes and degradation of cartilage. In the present study, hyaluronic acid/chitosan (HA/CS) nanoparticles were used as a vehicle for gene therapy of OA, and the cytokine response modifier A (CrmA) pDNA was proposed as the target gene. The HA/CS/pCrmA nanoparticles were prepared and the characteristics of the nanoparticles were examined. The nanoparticles were spherical, and the smallest size was obtained with the HA:CS weight ratio of 1:4. The release analysis exhibited a constant release over 29 days. The pDNA was completely combined with HA/CS nanoparticles and the HA/CS nanoparticles protected pDNA from degradation. Subsequently, rat synoviocytes were transfected with HA/CS/pDNA nanoparticles, and the results demonstrated that the HA/CS nanoparticles were able to improve the transfection capacity of pDNA. The cytotoxicity of the HA/CS/pDNA nanoparticles was additionally detected using a MTS assay to ensure that the HA/CS nanoparticle was a safe carrier. To additionally investigate the effects of HA/CS/pCrmA nanoparticles on synoviocytes in OA, the MMP‑3 and MMP‑13 gene expression levels were detected at the gene and protein expression levels. These results indicated that the HA/CS/pCrmA nanoparticles attenuated interleukin‑1β‑mediated inflammation in synoviocytes. It was concluded that the HA/CS/pCrmA nanoparticles may provide a novel approach to the treatment of OA.
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1 

Kiener HP, Ackermann J, Redlich K, Radda I, Steiner CW, Bitzan P, Smolen JS and Drach J: Interphase fluorescence in situ hybridization analysis of fibroblast-like synoviocytes of patients with rheumatoid arthritis and osteoarthritis. Arthritis Res Ther. 3(Suppl 2): P1192001. View Article : Google Scholar

2 

Abramson S, Attur M, Dave M, Leung M, Patel J, Gomez P and Amin A: Paracrine pathways of cartilage destruction in osteoarthritis. Arthritis Res Ther. 5(Suppl 3): S22003. View Article : Google Scholar

3 

Sun AR, Panchal SK, Friis T, Sekar S, Crawford R, Brown L, Xiao Y and Prasadam I: Obesity-associated metabolic syndrome spontaneously induces infiltration of pro-inflammatory macrophage in synovium and promotes osteoarthritis. PLoS One. 12:e01836932017. View Article : Google Scholar

4 

Goldring MB and Otero M: Inflammation in osteoarthritis. Curr Opin Rheumatol. 23:471–478. 2011. View Article : Google Scholar

5 

Wojdasiewicz P, Poniatowski LA and Szukiewicz D: The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis. Mediators Inflamm. 2014:5614592014. View Article : Google Scholar

6 

Suzuki M, Hashizume M, Yoshida H, Shiina M and Mihara M: IL-6 and IL-1 synergistically enhanced the production of MMPs from synovial cells by up-regulating IL-6 production and IL-1 receptor I expression. Cytokine. 51:178–183. 2010. View Article : Google Scholar

7 

Martel-Pelletier J, Boileau C, Pelletier JP and Roughley PJ: Cartilage in normal and osteoarthritis conditions. Best Prac Res Clin Rheumatol. 22:351–384. 2008. View Article : Google Scholar

8 

Liu-Bryan R: Synovium and the innate inflammatory network in osteoarthritis progression. Curr Rheumatol Rep. 15:3232013. View Article : Google Scholar

9 

Ginn SL, Alexander IE, Edelstein ML, Abedi MR and Wixon J: Gene therapy clinical trials worldwide to 2012 an update. J Gene Med. 15:65–77. 2013. View Article : Google Scholar

10 

Zhang Y, Wei H, Xu L, Yan G, Ma C, Yu M, Wei C and Sun Y: Preparation and evaluation of a non-viral gene vector for SiRNA: Multifunctional envelope-type nano device. Artif Cells Nanomed Biotechnol. 44:1259–1265. 2016.

11 

Yu B, Chen Y, Ouyang C, Huang H, Ji L and Chao H: A luminescent tetranuclear ruthenium(II) complex as a tracking non-viral gene vector. Chem Commun (Camb). 49:810–812. 2013. View Article : Google Scholar

12 

Malakooty Poor E, Baghaban Eslaminejad M, Gheibi N, Bagheri F and Atyabi F: Chitosan-pDNA nanoparticle characteristics determine the transfection efficacy of gene delivery to human mesenchymal stem cells. Artif Cells Nanomed Biotechnol. 42:376–384. 2014. View Article : Google Scholar

13 

Zhang X, Yu C, Xushi, Zhang C, Tang T and Dai K: Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo. Biochem Biophys Res Commun. 341:202–208. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Xu X, Qiu S, Zhang Y, Yin J and Min S: PELA microspheres with encapsulated arginine-chitosan/pBMP-2 nanoparticles induce pBMP-2 controlled-release, transfected osteoblastic progenitor cells, and promoted osteogenic differentiation. Artif Cells Nanomed Biotechnol. 45:330–339. 2017. View Article : Google Scholar

15 

Mansouri S, Cuie Y, Winnik F, Shi Q, Lavigne P, Benderdour M, Beaumont E and Fernandes JC: Characterization of folate-chitosan-DNA nanoparticles for gene therapy. Biomaterials. 27:2060–2065. 2006. View Article : Google Scholar

16 

Almond A: Hyaluronan. Cell Mol Life Sci. 64:1591–1596. 2007. View Article : Google Scholar

17 

Lu HD, Zhao HQ, Wang K and Lv LL: Novel hyaluronic acid-chitosan nanoparticles as non-viral gene delivery vectors targeting osteoarthritis. Int J Pharm. 420:358–365. 2011. View Article : Google Scholar

18 

Lu H, Lv L, Dai Y, Wu G, Zhao H and Zhang F: Porous chitosan scaffolds with embedded hyaluronic acid/chitosan/plasmid-DNA nanoparticles encoding TGF-β1 induce DNA controlled release, transfected chondrocytes, and promoted cell proliferation. PLoS One. 8:e699502013. View Article : Google Scholar

19 

Qiu B, Gong M, He QT and Zhou PH: Controlled release of interleukin-1 receptor antagonist from hyaluronic acid-chitosan microspheres attenuates interleukin-1 β-induced inflammation and apoptosis in chondrocytes. Biomed Res Int. 2016:62909572016. View Article : Google Scholar

20 

Ma BL, Zhou PH, Xie T, Shi L, Qiu B and Wang Q: Inhibition of interleukin-1beta-stimulated dedifferentiation of chondrocytes via controlled release of CrmA from hyaluronic acid-chitosan microspheres. BMC Musculoskelet Disord. 16:612015. View Article : Google Scholar : PubMed/NCBI

21 

Dobo J, Swanson R, Salvesen GS, Olson ST and Gettins PG: Cytokine response modifier a inhibition of initiator caspases results in covalent complex formation and dissociation of the caspase tetramer. J Biol Chem. 281:38781–38790. 2006. View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

23 

Zhou PH, Qiu B, Deng RH, Li HJ, Xu XF and Shang XF: Chondroprotective effects of hyaluronic acid-chitosan nanoparticles containing plasmid DNA encoding cytokine response modifier a in a rat knee osteoarthritis model. Cell Physiol Biochem. 47:1207–1216. 2018. View Article : Google Scholar

24 

Luangtana-Anan M, Limmatvapirat S, Nunthanid J, Chalongsuk R and Yamamoto K: Polyethylene glycol on stability of chitosan microparticulate carrier for protein. AAPS Pharmscitech. 11:1376–1382. 2010. View Article : Google Scholar

25 

Hou Y, Hu J, Park H and Lee M: Chitosan-based nanoparticles as a sustained protein release carrier for tissue engineering applications. J Biomed Mater Res A. 100:939–947. 2012. View Article : Google Scholar :

26 

Kang YM, Lee BN, Ko JH, Kim GH, Kang KN, Kim DY, Kim JH, Park YH, Chun HJ, Kim CH and Kim MS: In vivo biocompatibility study of electrospun chitosan microfiber for tissue engineering. Int J Mol Sci. 11:4140–4148. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Wang L and Stegemann JP: Thermogelling chitosan and collagen composite hydrogels initiated with beta-glycerophosphate for bone tissue engineering. Biomaterials. 31:3976–3985. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Masarudin MJ, Cutts SM, Pietersz GA, Evison BJ, Phillips DR and Pigram PJ: Factors determining the stability, size distribution, and cellular accumulation of small, monodisperse chitosan nanoparticles as candidate vectors for anticancer drug delivery: Application to the passive encapsulation of [(14) C]-doxorubicin. Nanotechnol Sci Appl. 9:47. 2016. View Article : Google Scholar

29 

Huang D, Wang R and Yang S: Cogels of hyaluronic acid and acellular matrix for cultivation of adipose-derived stem cells: Potential application for vocal fold tissue engineering. Biomed Res Int. 2016:65840542016. View Article : Google Scholar : PubMed/NCBI

30 

Shi J, Ma R, Wang L, Zhang J, Liu R, Li L, Liu Y, Hou L, Yu X, Gao J and Zhang Z: The application of hyaluronic acid-derivatized carbon nanotubes in hematoporphyrin monomethyl ether-based photodynamic therapy for in vivo and in vitro cancer treatment. Int J Nanomedicine. 8:2361–2373. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Huberlant S, Fernandez H, Vieille P, Khrouf M, Ulrich D, deTayrac R and Letouzey V: Application of a hyaluronic acid gel after intrauterine surgery may improve spontaneous fertility: A randomized controlled trial in new zealand white rabbits. Plos One. 10:e01256102015. View Article : Google Scholar : PubMed/NCBI

32 

Kim Y and Kumar S: CD44-mediated adhesion to hyaluronic acid contributes to mechanosensing and invasive motility. Mol Cancer Res. 12:1416–1429. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Contreras-Ruiz L, de la Fuente M, Parraga JE, López-García A, Fernández I, Seijo B, Sánchez A, Calonge M and Diebold Y: Intracellular trafficking of hyaluronic acid-chitosan oligomer-based nanoparticles in cultured human ocular surface cells. Mol Vis. 17:279–290. 2011.

34 

Centelles MN, Qian C, Campanero MA and Irache JM: New methodologies to characterize the effectiveness of the gene transfer mediated by DNA-chitosan nanoparticles. Int J Nanomedicine. 3:451–460. 2008.

35 

Chen Y, Mohanraj VJ, Wang F and Benson HA: Designing chitosan-dextran sulfate nanoparticles using charge ratios. AAPS Pharmscitech. 8:E982007. View Article : Google Scholar

36 

Oliverira AV, Silva AP, Bitoque DB, Silva GA and Rosa da Costa AM: Transfection efficiency of chitosan and thiolated chitosan in retinal pigment epithelium cells: A comparative study. J Pharm Bioallied Sci. 5:111–118. 2013. View Article : Google Scholar

37 

Granot E, Shouval D and Ashur Y: Cell adhesion molecules and hyaluronic acid as markers of inflammation, fibrosis and response to antiviral therapy in chronic hepatitis C patients. Mediators Inflamm. 10:253–258. 2001. View Article : Google Scholar

38 

Khurana SS, Riehl TE, Moore BD, Fassan M, Rugge M, Romero-Gallo J, Noto J, Peek RM Jr, Stenson WF and Mills JC: The hyaluronic acid receptor CD44 coordinates normal and metaplastic gastric epithelial progenitor cell proliferation. J Biol Chem. 288:16085–16097. 2013. View Article : Google Scholar

39 

Wang T, Hou J, Su C, Zhao L and Shi Y: Hyaluronic acid-coated chitosan nanoparticles induce ROS-mediated tumor cell apoptosis and enhance antitumor efficiency by targeted drug delivery via CD44. J Nanobiotechnology. 15:72017. View Article : Google Scholar :

40 

Aborehab NM, El Bishbishy MH, Refaiy A and Waly NE: A putative Chondroprotective role for IL-1 beta and MPO in herbal treatment of experimental osteoarthritis. BMC Complement Altern Med. 17:4952017. View Article : Google Scholar

41 

Afif H, Benderdour M, Mfuna-Endam L, Martel-Pelletier J, Pelletier JP, Duval N and Fahmi H: Peroxisome proliferator-activated receptor gamma 1 expression is diminished in human osteoarthritis cartilage and is downregulated by IL-1 beta in articular chondrocytes. Arthritis Res Ther. 9:R312007. View Article : Google Scholar

42 

Chia WT, Yeh LT, Chen YW, Lee HS, Chang DM and Sytwu HK: IL-1 beta in irrigation fluid and mRNA expression in synovial tissue of the knee joint as therapeutic markers of inflammation in collagen antibody-induced arthritis. Dis Markers. 26:1–7. 2009. View Article : Google Scholar

43 

Ekert PG, Silke J and Vaux DL: Inhibition of apoptosis and clonogenic survival of cells expressing crmA variants: Optimal caspase substrates are not necessarily optimal inhibitors. EMBO J. 18:330–338. 1999. View Article : Google Scholar : PubMed/NCBI

44 

Mayer-Barber KD, Barber DL, Shenderov K, White SD, Wilson MS, Cheever A, Kugler D, Hieny S, Caspar P, Núñez G, et al: Cutting edge: Caspase-1 independent IL-1 beta production is critical for host resistance to mycobacterium tuberculosis and does not require TLR signaling in vivo. J Immunol. 184:3326–3330. 2010. View Article : Google Scholar

45 

Wang F and He X: Intra-articular hyaluronic acid and corticosteroids in the treatment of knee osteoarthritis: A meta-analysis. Exp Ther Med. 9:493–500. 2015. View Article : Google Scholar : PubMed/NCBI

46 

Georgopoulou A, Kaliva M, Vamvakaki M and Chatzinikolaidou M: Osteogenic potential of pre-osteoblastic cells on a chitosan-graft-polycaprolactone copolymer. Materials (Basel). 11:E4902018. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Qiu B, Xu XF, Deng RH, Xia GQ, Shang XF and Zhou PH: Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro. Int J Mol Med 43: 1076-1084, 2019.
APA
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., & Zhou, P. (2019). Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro. International Journal of Molecular Medicine, 43, 1076-1084. https://doi.org/10.3892/ijmm.2018.3997
MLA
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., Zhou, P."Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro". International Journal of Molecular Medicine 43.2 (2019): 1076-1084.
Chicago
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., Zhou, P."Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro". International Journal of Molecular Medicine 43, no. 2 (2019): 1076-1084. https://doi.org/10.3892/ijmm.2018.3997
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu B, Xu XF, Deng RH, Xia GQ, Shang XF and Zhou PH: Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro. Int J Mol Med 43: 1076-1084, 2019.
APA
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., & Zhou, P. (2019). Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro. International Journal of Molecular Medicine, 43, 1076-1084. https://doi.org/10.3892/ijmm.2018.3997
MLA
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., Zhou, P."Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro". International Journal of Molecular Medicine 43.2 (2019): 1076-1084.
Chicago
Qiu, B., Xu, X., Deng, R., Xia, G., Shang, X., Zhou, P."Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in vitro". International Journal of Molecular Medicine 43, no. 2 (2019): 1076-1084. https://doi.org/10.3892/ijmm.2018.3997
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