Crude protein from spirulina increases the viability of CCD‑986sk cells via the EGFR/MAPK signaling pathway

Liu,Ping; Lee,Min‑Kyeong; Choi,Jeong‑Wook; Choi,Youn‑Hee; Nam,Taek‑Jeong

doi:10.3892/ijmm.2018.4025

Crude protein from spirulina increases the viability of CCD‑986sk cells via the EGFR/MAPK signaling pathway

Authors:
- Ping Liu
- Min‑Kyeong Lee
- Jeong‑Wook Choi
- Youn‑Hee Choi
- Taek‑Jeong Nam
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Affiliations: Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea, Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Republic of Korea
Published online on: December 11, 2018 https://doi.org/10.3892/ijmm.2018.4025
Pages: 771-778
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Spirulina, an edible blue‑green alga, has great potential for various applications in human health, possibly including reduced skin aging. The mechanisms by which spirulina crude protein (SPCP) may influence human skin fibroblast viability are not yet understood; therefore, a human dermal fibroblast cell line (CCD‑986sk) was used as a cell model system to study the influence of SPCP on human skin fibroblast viability. An enzyme‑linked immunosorbent assay showed that collagen formation improved in SPCP‑treated cells in a dose‑dependent manner, while elastase activity was decreased. In addition, western blot analysis showed a dose‑dependent decrease in the expression of the aging‑associated gene matrix metalloproteinase‑8, a collagen‑degradative enzyme. It was also shown that SPCP upregulated epidermal growth factor receptor (EGFR) activity, leading to activation of the mitogen‑activated protein kinase (MAPK)/extracellular signal‑regulated kinase (ERK) signaling pathway. Together, these results demonstrated that SPCP increases human fibroblast viability by activation of the EGFR/MAPK signaling pathway. This contribution sheds light on the molecular mechanism for SPCP increasing the viability of human skin cell and provides a potential efficient cosmeceutical for protecting human skin.

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