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Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro

  • Authors:
    • Yang Xu
    • Ping Sun
    • Jian‑Yu Wang
    • Zong‑Ze Li
    • Rui‑Lan Gao
    • Xue‑Zhe Wang
    • William D. Phillips
    • Simon X. Liang
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Hematology, Jining First People's Hospital, Jining, Shandong 272000, P.R. China, Institution of Hematology Research, The First Affiliated Hospital of Zhejian Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China, Department of Clinical Laboratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, School of Medical Sciences (Physiology) and Bosch Institute, University of Sydney, Sydney, NSW 2006, Australia
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1128-1138
    |
    Published online on: January 8, 2019
       https://doi.org/10.3892/ijmm.2019.4053
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Abstract

Side population (SP) cells are a small subpopulation of cells found in many mammalian tissues and organs, identified by their capacity to efflux Hoechst 33342 dye. They are enriched for stem/progenitor cell activity. SP cells isolated from the adult mouse lung can be separated into a CD45+ subset (bone marrow‑derived) and a CD45‑ subset that can be subdivided into CD31‑ and CD31+ subpopulations. CD45‑/CD31‑ lung SP (LSP) cells are known to be mesenchymal stem cells. However, CD45‑/CD31+ LSP cells are not fully characterized. In the present study, it was found that CD45‑/CD31+ LSP cells were able to form colonies. Based on the expression of vascular endothelial growth factor receptor 2 (VEGFR2), these cells were separated into VEGFR2‑ and VEGFR2+ cells. The CD45‑/CD31+/VEGFR2‑ LSP cells expressed genes characteristic of smooth muscle and endothelial progenitors, and were able to differentiate into smooth muscle and endothelial cells in vitro. The CD45‑/CD31+/VEGFR2+ LSP cells expressed genes characteristic of endothelial progenitors and gave rise to endothelial cells, although not smooth muscle, in vitro. The data demonstrate that CD45‑/CD31+/VEGFR2‑ LSP cells differentiated into CD45‑/CD31+/VEGFR2+ LSP cells and then endothelial cells, indicating that CD45‑/CD31+/VEGFR2+ LSP cells are likely to be derived from CD45‑/CD31+/VEGFR2‑ LSP cells. Taken together, the results suggest that CD45‑/CD31+ LSP cells can be separated into CD45‑/CD31+/VEGFR2‑ LSP cells, which may be progenitors of endothelial and smooth muscle, whereas CD45‑/CD31+/VEGFR2+ LSP cells may serve as late commitment endothelial progenitors in the adult mouse lung.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Sun P, Wang JY, Li ZZ, Gao RL, Wang XZ, Phillips WD and Liang SX: Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43: 1128-1138, 2019.
APA
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X. ... Liang, S.X. (2019). Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. International Journal of Molecular Medicine, 43, 1128-1138. https://doi.org/10.3892/ijmm.2019.4053
MLA
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X., Phillips, W. D., Liang, S. X."Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro". International Journal of Molecular Medicine 43.3 (2019): 1128-1138.
Chicago
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X., Phillips, W. D., Liang, S. X."Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro". International Journal of Molecular Medicine 43, no. 3 (2019): 1128-1138. https://doi.org/10.3892/ijmm.2019.4053
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Sun P, Wang JY, Li ZZ, Gao RL, Wang XZ, Phillips WD and Liang SX: Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43: 1128-1138, 2019.
APA
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X. ... Liang, S.X. (2019). Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. International Journal of Molecular Medicine, 43, 1128-1138. https://doi.org/10.3892/ijmm.2019.4053
MLA
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X., Phillips, W. D., Liang, S. X."Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro". International Journal of Molecular Medicine 43.3 (2019): 1128-1138.
Chicago
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X., Phillips, W. D., Liang, S. X."Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro". International Journal of Molecular Medicine 43, no. 3 (2019): 1128-1138. https://doi.org/10.3892/ijmm.2019.4053
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