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Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica

  • Authors:
    • Chunsheng Yang
    • Yang Yang
    • Li Ma
    • Guang‑Xian Zhang
    • Fu‑Dong Shi
    • Yaping Yan
    • Guoqiang Chang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300052, P.R. China, Department of Neurosurgery and Neuro‑Oncology, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China, Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1395-1405
    |
    Published online on: January 9, 2019
       https://doi.org/10.3892/ijmm.2019.4056
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Abstract

Neuromyelitis optica (NMO) is a refractory autoimmune inflammatory disease of the central nervous system without an effective cure. Autologous bone marrow‑derived mesenchymal stem cells (BM‑MSCs) are considered to be promising therapeutic agents for this disease due to their potential regenerative, immune regulatory and neurotrophic effects. However, little is known about the cytological features of BM‑MSCs from patients with NMO, which may influence any therapeutic effects. The present study aimed to compare the proliferation, differentiation and senescence of BM‑MSCs from patients with NMO with that of age‑ and sex‑matched healthy subjects. It was revealed that there were no significant differences in terms of cell morphology or differentiation capacities in the BM‑MSCs from the patients with NMO. However, in comparison with healthy controls, BM‑MSCs derived from the Patients with NMO exhibited a decreased proliferation rate, in addition to a decreased expression of several cell cycle‑promoting and proliferation‑associated genes. Furthermore, the cell death rate increased in BM‑MSCs from patients under normal culture conditions and an assessment of the gene expression profile further confirmed that the BM‑MSCs from patients with NMO were more vulnerable to senescence. Platelet‑derived growth factor (PDGF), as a major mitotic stimulatory factor for MSCs and a potent therapeutic cytokine in demyelinating disease, was able to overcome the decreased proliferation rate and increased senescence defects in BM‑MSCs from the patients with NMO. Taken together, the results from the present study have enabled the proposition of the possibility of combining the application of autologous BM‑MSCs and PDGF for refractory and severe patients with NMO in order to elicit improved therapeutic effects, or, at the least, to include PDGF as a necessary and standard growth factor in the current in vitro formula for the culture of NMO patient‑derived BM‑MSCs.
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Spandidos Publications style
Yang C, Yang Y, Ma L, Zhang GX, Shi FD, Yan Y and Chang G: Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica . Int J Mol Med 43: 1395-1405, 2019.
APA
Yang, C., Yang, Y., Ma, L., Zhang, G., Shi, F., Yan, Y., & Chang, G. (2019). Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica . International Journal of Molecular Medicine, 43, 1395-1405. https://doi.org/10.3892/ijmm.2019.4056
MLA
Yang, C., Yang, Y., Ma, L., Zhang, G., Shi, F., Yan, Y., Chang, G."Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica ". International Journal of Molecular Medicine 43.3 (2019): 1395-1405.
Chicago
Yang, C., Yang, Y., Ma, L., Zhang, G., Shi, F., Yan, Y., Chang, G."Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica ". International Journal of Molecular Medicine 43, no. 3 (2019): 1395-1405. https://doi.org/10.3892/ijmm.2019.4056
Copy and paste a formatted citation
x
Spandidos Publications style
Yang C, Yang Y, Ma L, Zhang GX, Shi FD, Yan Y and Chang G: Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica . Int J Mol Med 43: 1395-1405, 2019.
APA
Yang, C., Yang, Y., Ma, L., Zhang, G., Shi, F., Yan, Y., & Chang, G. (2019). Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica . International Journal of Molecular Medicine, 43, 1395-1405. https://doi.org/10.3892/ijmm.2019.4056
MLA
Yang, C., Yang, Y., Ma, L., Zhang, G., Shi, F., Yan, Y., Chang, G."Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica ". International Journal of Molecular Medicine 43.3 (2019): 1395-1405.
Chicago
Yang, C., Yang, Y., Ma, L., Zhang, G., Shi, F., Yan, Y., Chang, G."Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica ". International Journal of Molecular Medicine 43, no. 3 (2019): 1395-1405. https://doi.org/10.3892/ijmm.2019.4056
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