Open Access

Pristimerin enhances the effect of cisplatin by inhibiting the miR‑23a/Akt/GSK3β signaling pathway and suppressing autophagy in lung cancer cells

  • Authors:
    • Yingbing Zhang
    • Jiquan Wang
    • Beina Hui
    • Wenze Sun
    • Bin Li
    • Fan Shi
    • Shaomin Che
    • Linyan Chai
    • Liping Song
  • View Affiliations

  • Published online on: January 10, 2019     https://doi.org/10.3892/ijmm.2019.4057
  • Pages: 1382-1394
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lung cancer is a common type of cancer with a high mortality rate in China. Cisplatin (Cis) is one of the most effective broad‑spectrum chemotherapeutic drugs for the treatment of advanced lung cancer. However, Cis resistance remains an obstacle in the treatment of advanced lung cancer. Pristimerin (Pris), a naturally occurring triterpenoid quinone compound, not only possesses anticancer properties, but also enhances chemosensitivity. Therefore, the present study aimed to investigate whether Pris can enhance the chemosensitivity of lung cancer cells to Cis and identify the underlying mechanism. A Cell Counting kit‑8 and flow cytometry were used to determine cell viability, cell cycle progression and apoptosis in A549 and NCI‑H446 cells. Western blotting was used to determine cell apoptosis‑related, cell cycle‑related and autophagy‑related proteins. The results showed that Pris inhibited cell proliferation, and induced G0/G1 arrest and cell apoptosis in A549 and NCI‑H446 cells. The western blotting revealed that Pris effectively synergized with Cis to induce cell apoptosis by inhibiting the microRNA‑23a/Akt/glycogen synthase kinase 3β signaling pathway and suppressing autophagy. In vivo xenograft experiments confirmed that Pris effectively synergized with Cis to suppress tumor growth. Collectively, these results indicate that Pris synergized with Cis and that this may be a potential therapeutic strategy to overcome lung cancer.
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March-2019
Volume 43 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang Y, Wang J, Hui B, Sun W, Li B, Shi F, Che S, Chai L and Song L: Pristimerin enhances the effect of cisplatin by inhibiting the miR‑23a/Akt/GSK3β signaling pathway and suppressing autophagy in lung cancer cells. Int J Mol Med 43: 1382-1394, 2019
APA
Zhang, Y., Wang, J., Hui, B., Sun, W., Li, B., Shi, F. ... Song, L. (2019). Pristimerin enhances the effect of cisplatin by inhibiting the miR‑23a/Akt/GSK3β signaling pathway and suppressing autophagy in lung cancer cells. International Journal of Molecular Medicine, 43, 1382-1394. https://doi.org/10.3892/ijmm.2019.4057
MLA
Zhang, Y., Wang, J., Hui, B., Sun, W., Li, B., Shi, F., Che, S., Chai, L., Song, L."Pristimerin enhances the effect of cisplatin by inhibiting the miR‑23a/Akt/GSK3β signaling pathway and suppressing autophagy in lung cancer cells". International Journal of Molecular Medicine 43.3 (2019): 1382-1394.
Chicago
Zhang, Y., Wang, J., Hui, B., Sun, W., Li, B., Shi, F., Che, S., Chai, L., Song, L."Pristimerin enhances the effect of cisplatin by inhibiting the miR‑23a/Akt/GSK3β signaling pathway and suppressing autophagy in lung cancer cells". International Journal of Molecular Medicine 43, no. 3 (2019): 1382-1394. https://doi.org/10.3892/ijmm.2019.4057