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International Journal of Molecular Medicine
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Article

Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy

  • Authors:
    • Tae Woong Hwang
    • Dong Hun Kim
    • Da Bi Kim
    • Tae Won Jang
    • Gun‑Hwa Kim
    • Minho Moon
    • Kyung Ah Yoon
    • Dae Eun Choi
    • Jae Ho Park
    • Jwa‑Jin Kim
  • View Affiliations / Copyright

    Affiliations: Department of Medical Science, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea, Department of Medicinal Plant Resources, Andong National University, Andong, Gyeongsangbuk 36729, Republic of Korea, Drug and Disease Target Team, Division of Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Chungcheong 28119, Republic of Korea, Department of Biochemistry, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea, Department of Clinical Laboratory Science, Daejeon Health Sciences College, Daejeon 34504, Republic of Korea, Department of Nephrology, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea, Department of Pharmaceutical Science, Jungwon University, Geosan, Chungbuk 28024, Republic of Korea
  • Pages: 1478-1486
    |
    Published online on: January 11, 2019
       https://doi.org/10.3892/ijmm.2019.4061
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Abstract

Temozolomide (TMZ) is an alkylating agent commonly used as a first‑line treatment for high‑grade glioblastoma. However, TMZ has short half‑life and frequently induces tumor resistance, which can limit its therapeutic efficiency. In the present study, it was hypothesized that combined treatment with TMZ and acteoside has synergistic effects in glioblastoma therapy. Using cell viability and wound‑healing assays, it was determined that this treatment regimen reduced cell viability and migration to a greater extent than either TMZ or acteoside alone. Following previous reports that TMZ affected autophagy in glioma cells, the present study examined the effects of TMZ + acteoside combination treatment on apoptosis and autophagy. The TMZ + acteoside combination treatment increased the cleavage of caspase‑3 and levels of B‑cell lymphoma 2 (Bcl‑2)‑associated X protein and phosphorylated p53, and decreased the level of Bcl‑2. The combination treatment increased microtubule‑associated protein 1 light chain 3 and apoptosis‑related gene expression. It was also determined that TMZ + acteoside induced apoptosis and autophagy through the mitogen‑activated protein kinase signaling pathway. These findings suggest that acteoside has beneficial effects on TMZ‑based glioblastoma therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Hwang TW, Kim DH, Kim DB, Jang TW, Kim GH, Moon M, Yoon KA, Choi DE, Park JH, Kim JJ, Kim JJ, et al: Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy. Int J Mol Med 43: 1478-1486, 2019.
APA
Hwang, T.W., Kim, D.H., Kim, D.B., Jang, T.W., Kim, G., Moon, M. ... Kim, J. (2019). Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy. International Journal of Molecular Medicine, 43, 1478-1486. https://doi.org/10.3892/ijmm.2019.4061
MLA
Hwang, T. W., Kim, D. H., Kim, D. B., Jang, T. W., Kim, G., Moon, M., Yoon, K. A., Choi, D. E., Park, J. H., Kim, J."Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy". International Journal of Molecular Medicine 43.3 (2019): 1478-1486.
Chicago
Hwang, T. W., Kim, D. H., Kim, D. B., Jang, T. W., Kim, G., Moon, M., Yoon, K. A., Choi, D. E., Park, J. H., Kim, J."Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy". International Journal of Molecular Medicine 43, no. 3 (2019): 1478-1486. https://doi.org/10.3892/ijmm.2019.4061
Copy and paste a formatted citation
x
Spandidos Publications style
Hwang TW, Kim DH, Kim DB, Jang TW, Kim GH, Moon M, Yoon KA, Choi DE, Park JH, Kim JJ, Kim JJ, et al: Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy. Int J Mol Med 43: 1478-1486, 2019.
APA
Hwang, T.W., Kim, D.H., Kim, D.B., Jang, T.W., Kim, G., Moon, M. ... Kim, J. (2019). Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy. International Journal of Molecular Medicine, 43, 1478-1486. https://doi.org/10.3892/ijmm.2019.4061
MLA
Hwang, T. W., Kim, D. H., Kim, D. B., Jang, T. W., Kim, G., Moon, M., Yoon, K. A., Choi, D. E., Park, J. H., Kim, J."Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy". International Journal of Molecular Medicine 43.3 (2019): 1478-1486.
Chicago
Hwang, T. W., Kim, D. H., Kim, D. B., Jang, T. W., Kim, G., Moon, M., Yoon, K. A., Choi, D. E., Park, J. H., Kim, J."Synergistic anticancer effect of acteoside and temozolomide-based glioblastoma chemotherapy". International Journal of Molecular Medicine 43, no. 3 (2019): 1478-1486. https://doi.org/10.3892/ijmm.2019.4061
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