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Article

Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies

  • Authors:
    • Rui‑Qi Wang
    • Yu‑Jie Wang
    • Zhi‑Qiang Xu
    • Yan‑Jun Zhou
    • Meng‑Da Cao
    • Wei Zhu
    • Jin‑Lyu Sun
    • Ji‑Fu Wei
  • View Affiliations / Copyright

    Affiliations: Department of Allergy, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing Key Laboratory of Precision Medicine for Diagnosis and Treatment on Allergic Diseases, Beijing 100730, P.R. China, Research Division of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 1531-1541
    |
    Published online on: January 15, 2019
       https://doi.org/10.3892/ijmm.2019.4065
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Abstract

Dogs are a major source of indoor allergens. However, the prevalence of dog allergies in China remains unclear, especially in children. In the present study, Can f 7, a canine allergen belonging to the Niemann pick type C2 protein family, was selected to study its sensitization rate in Chinese children with dog allergies. The Can f 7 gene was subcloned into a pET‑28a vector and expressed in Escherichia coli BL21 (DE3) cells. Recombinant Can f 7 was purified by nickel affinity chromatography, identified by SDS‑PAGE electrophoresis, and had its allergenicity assessed by western blot, ELISA and basophil activation tests. Through a series of bioinformatical approaches, B‑cell epitopes, secondary structures, and 3 dimensional (3D) homology modeling of Can f 7 were predicted. The activity of the B cell epitopes was verified by ELISA. The recombinant Can f 7 showed a distinct band with a molecular weight of 14 kDa. Six of 20 sera from dog‑allergic children reacted positively to the Can f 7. Can f 7 induced an ~4.0‑fold increase in cluster of differentiation 63 and C‑C motif chemokine receptor R3 expression in basophils sensitized with the serum of dog‑allergic children compared with those of non‑allergic controls. The secondary structure analysis showed that Can f 7 contains 6 β‑sheets. Five B cell epitopes of Can f 7 were predicted, and two of these were confirmed by ELISA. These results indicate that Can f 7 is an important canine allergen in Chinese children and provide novel data for further research concerning the use of Can f 7 in the diagnosis and treatment of Chinese children with canine allergy symptoms.
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Copy and paste a formatted citation
Spandidos Publications style
Wang RQ, Wang YJ, Xu ZQ, Zhou YJ, Cao MD, Zhu W, Sun JL and Wei JF: Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies. Int J Mol Med 43: 1531-1541, 2019.
APA
Wang, R., Wang, Y., Xu, Z., Zhou, Y., Cao, M., Zhu, W. ... Wei, J. (2019). Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies. International Journal of Molecular Medicine, 43, 1531-1541. https://doi.org/10.3892/ijmm.2019.4065
MLA
Wang, R., Wang, Y., Xu, Z., Zhou, Y., Cao, M., Zhu, W., Sun, J., Wei, J."Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies". International Journal of Molecular Medicine 43.3 (2019): 1531-1541.
Chicago
Wang, R., Wang, Y., Xu, Z., Zhou, Y., Cao, M., Zhu, W., Sun, J., Wei, J."Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies". International Journal of Molecular Medicine 43, no. 3 (2019): 1531-1541. https://doi.org/10.3892/ijmm.2019.4065
Copy and paste a formatted citation
x
Spandidos Publications style
Wang RQ, Wang YJ, Xu ZQ, Zhou YJ, Cao MD, Zhu W, Sun JL and Wei JF: Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies. Int J Mol Med 43: 1531-1541, 2019.
APA
Wang, R., Wang, Y., Xu, Z., Zhou, Y., Cao, M., Zhu, W. ... Wei, J. (2019). Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies. International Journal of Molecular Medicine, 43, 1531-1541. https://doi.org/10.3892/ijmm.2019.4065
MLA
Wang, R., Wang, Y., Xu, Z., Zhou, Y., Cao, M., Zhu, W., Sun, J., Wei, J."Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies". International Journal of Molecular Medicine 43.3 (2019): 1531-1541.
Chicago
Wang, R., Wang, Y., Xu, Z., Zhou, Y., Cao, M., Zhu, W., Sun, J., Wei, J."Canis familiaris allergen Can f 7: Expression, purification and analysis of B cell epitopes in Chinese children with dog allergies". International Journal of Molecular Medicine 43, no. 3 (2019): 1531-1541. https://doi.org/10.3892/ijmm.2019.4065
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