Open Access

miR‑122 and miR‑199 synergistically promote autophagy in oral lichen planus by targeting the Akt/mTOR pathway

  • Authors:
    • Liang Wang
    • Wei Wu
    • Jijun Chen
    • Youhua Li
    • Ming Xu
    • Yawei Cai
  • View Affiliations

  • Published online on: January 21, 2019     https://doi.org/10.3892/ijmm.2019.4068
  • Pages: 1373-1381
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to characterize the roles of two microRNAs (miRNAs), miR‑122 and miR‑199, in oral lichen planus (OLP). miRNA microarray analysis was performed to detect potential miRNAs involved in OLP, while in‑silicon analysis, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blot and immunohistochemistry (IHC) analyses were utilized to explore the molecular mechanisms underlying the roles of miR‑199 and miR‑122 in OLP. The results from the microarray and RT‑qPCR analyses demonstrated that the expression levels of miR‑122 and miR‑199 were significantly decreased in the peripheral blood mononuclear cells (PBMCs) collected from the OLP group compared with the control group. In addition, miR‑122 and miR‑199 directly targeted AKT serine/threonine kinase 1 (AKT1) and mammalian target of rapamycin (mTOR), respectively, by binding to their 3' UTRs. AKT1 and mTOR were highly expressed in PBMCs derived from OLP patients. In fact, a negative regulatory relationship was observed between miR‑122 and AKT1, and between miR‑199 and mTOR, with negative correlation coefficients of ‑0.41 and ‑0.51, respectively. Furthermore, the protein levels of AKT1, mTOR and microtubule associated protein 1 light chain 3β (LC3B) were upregulated in the OLP group compared with the control group. Finally, overexpression of miR‑122 inhibited the expression of AKT1 and LC3B, while overexpression of miR‑199 reduced the levels of mTOR and LC3B. In conclusion, the present study demonstrated that miR‑199 and miR‑122 are implicated in the pathogenesis of OLP by regulating the expression of mTOR and AKT1.
View Figures
View References

Related Articles

Journal Cover

March-2019
Volume 43 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Wu W, Chen J, Li Y, Xu M and Cai Y: miR‑122 and miR‑199 synergistically promote autophagy in oral lichen planus by targeting the Akt/mTOR pathway . Int J Mol Med 43: 1373-1381, 2019.
APA
Wang, L., Wu, W., Chen, J., Li, Y., Xu, M., & Cai, Y. (2019). miR‑122 and miR‑199 synergistically promote autophagy in oral lichen planus by targeting the Akt/mTOR pathway . International Journal of Molecular Medicine, 43, 1373-1381. https://doi.org/10.3892/ijmm.2019.4068
MLA
Wang, L., Wu, W., Chen, J., Li, Y., Xu, M., Cai, Y."miR‑122 and miR‑199 synergistically promote autophagy in oral lichen planus by targeting the Akt/mTOR pathway ". International Journal of Molecular Medicine 43.3 (2019): 1373-1381.
Chicago
Wang, L., Wu, W., Chen, J., Li, Y., Xu, M., Cai, Y."miR‑122 and miR‑199 synergistically promote autophagy in oral lichen planus by targeting the Akt/mTOR pathway ". International Journal of Molecular Medicine 43, no. 3 (2019): 1373-1381. https://doi.org/10.3892/ijmm.2019.4068