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Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells

  • Authors:
    • Jing Cai
    • Jiangman Chen
    • Huanxu Guo
    • Yaping Pan
    • Yibo Zhang
    • Wei Zhao
    • Xin Li
    • Yonggang Li
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics and Oral Biology, School of Stomatology, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Periodontics and Oral Biology, School of Stomatology, China Medical University, Shenyang, Liaoning 110002, P.R. China, Department of Pathogeny Biology, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1430-1440
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    Published online on: January 21, 2019
       https://doi.org/10.3892/ijmm.2019.4069
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Abstract

Porphyromonas gingivalis (P. gingivalis) is a periodontal pathogen that may accumulate with other organisms in subgingival plaque biofilms and is associated with periodontal disease. P. gingivalis fimbriae (FimA) is a filamentous structure on the surface of bacteria that is closely associated with bacterial adhesion to and colonization of host tissues, and serves an essential role in biofilm formation. The present study aimed to construct P. gingivalis FimA prokaryotic expression plasmids, purify a FimA fusion protein and explore the effect of a recombinant FimA protein on the inflammatory response in human peripheral blood mononuclear cells (PBMCs). P. gingivalis FimA prokaryotic expression plasmids were constructed by gene cloning and recombination technology. SDS‑PAGE was used to evaluate the purified recombinant FimA protein. The cell proliferation rate and inflammatory cytokine expression of PBMCs treated with the FimA fusion protein with or without transfection with toll‑like receptor 4 (TLR4) small interfering (si)RNA were detected by CCK‑8 assays and ELISAs, respectively. The expression levels of TLR4, nuclear factor kappa‑light‑chain‑enhancer of activated B cells (NF‑κB) and myeloid differentiation primary response 88 (MyD88) in PBMCs were detected by western blot analysis and reverse transcription quantitative polymerase chain reaction. A FimA fusion protein with high purity was obtained. FimA fusion protein treatment significantly increased PBMC proliferation and promoted the release of tumor necrosis factor‑α (TNF‑α), interleukin (IL)‑6, matrix metalloproteinase (MMP)‑8 and MMP‑9 in PBMCs. TLR4 interference reversed the effects of the FimA fusion protein on PBMC proliferation and inflammatory cytokine release. The expression levels of TLR4, NF‑κB and MyD88 in PBMCs were significantly increased following treatment with the FimA fusion protein, while the expression levels of these genes at the mRNA and protein levels decreased significantly in PBMCs following FimA fusion protein treatment and TLR4 interference. The FimA fusion protein increased PBMC proliferation and promoted the release of the inflammatory cytokines TNF‑α, IL‑6, MMP‑8 and MMP‑9 via the TLR4/NF‑κB signaling pathway. FimA may serve as a promising therapeutic strategy for periodontal disease.
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Copy and paste a formatted citation
Spandidos Publications style
Cai J, Chen J, Guo H, Pan Y, Zhang Y, Zhao W, Li X and Li Y: Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells . Int J Mol Med 43: 1430-1440, 2019.
APA
Cai, J., Chen, J., Guo, H., Pan, Y., Zhang, Y., Zhao, W. ... Li, Y. (2019). Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells . International Journal of Molecular Medicine, 43, 1430-1440. https://doi.org/10.3892/ijmm.2019.4069
MLA
Cai, J., Chen, J., Guo, H., Pan, Y., Zhang, Y., Zhao, W., Li, X., Li, Y."Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells ". International Journal of Molecular Medicine 43.3 (2019): 1430-1440.
Chicago
Cai, J., Chen, J., Guo, H., Pan, Y., Zhang, Y., Zhao, W., Li, X., Li, Y."Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells ". International Journal of Molecular Medicine 43, no. 3 (2019): 1430-1440. https://doi.org/10.3892/ijmm.2019.4069
Copy and paste a formatted citation
x
Spandidos Publications style
Cai J, Chen J, Guo H, Pan Y, Zhang Y, Zhao W, Li X and Li Y: Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells . Int J Mol Med 43: 1430-1440, 2019.
APA
Cai, J., Chen, J., Guo, H., Pan, Y., Zhang, Y., Zhao, W. ... Li, Y. (2019). Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells . International Journal of Molecular Medicine, 43, 1430-1440. https://doi.org/10.3892/ijmm.2019.4069
MLA
Cai, J., Chen, J., Guo, H., Pan, Y., Zhang, Y., Zhao, W., Li, X., Li, Y."Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells ". International Journal of Molecular Medicine 43.3 (2019): 1430-1440.
Chicago
Cai, J., Chen, J., Guo, H., Pan, Y., Zhang, Y., Zhao, W., Li, X., Li, Y."Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells ". International Journal of Molecular Medicine 43, no. 3 (2019): 1430-1440. https://doi.org/10.3892/ijmm.2019.4069
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