Open Access

miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells

  • Authors:
    • Tao Han
    • Ni Wu
    • Youjing Wang
    • Weimin Shen
    • Jijun Zou
  • View Affiliations

  • Published online on: January 21, 2019     https://doi.org/10.3892/ijmm.2019.4070
  • Pages: 1441-1451
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs) post‑transcriptionally regulate gene expression by targeting the 3' untranslated region (UTR) of target genes, and serve diverse roles in cell proliferation, differentiation and apoptosis. However, the association between miR‑16‑2‑3p and 3‑phosphoinositide‑dependent protein kinase‑1 (PDPK1) in nonsyndromic cleft lip (NSCL) remains unclear. In the present study, a luciferase activity assay indicated that miR‑16‑2‑3p negatively regulated PDPK1 in maxillary primordium mesenchymal cells (MPMCs). In addition, it was confirmed that the expression levels of miR‑16‑2‑3p was markedly increased in cleft lip tissues compared with those in adjacent normal lip tissues. A negative correlation between miR‑16‑2‑3p and PDPK1 in cleft lip tissues was observed. Furthermore, miR‑16‑2‑3p inhibited cell proliferation and migration, and induced apoptosis of MPMCs via repressing PDPK1. Finally, miR‑16‑2‑3p exerted its suppressive role in MPMCs by inhibiting the PDPK1/protein kinase B signaling pathway. These results indicate that miR‑16‑2‑3p may inhibit cell proliferation and migration, and promote apoptosis in MPMCs through repression of PDPK1 and may be a potential target for future clinical prevention and treatment of NSCL.
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March-2019
Volume 43 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Han T, Wu N, Wang Y, Shen W and Zou J: miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells . Int J Mol Med 43: 1441-1451, 2019
APA
Han, T., Wu, N., Wang, Y., Shen, W., & Zou, J. (2019). miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells . International Journal of Molecular Medicine, 43, 1441-1451. https://doi.org/10.3892/ijmm.2019.4070
MLA
Han, T., Wu, N., Wang, Y., Shen, W., Zou, J."miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells ". International Journal of Molecular Medicine 43.3 (2019): 1441-1451.
Chicago
Han, T., Wu, N., Wang, Y., Shen, W., Zou, J."miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells ". International Journal of Molecular Medicine 43, no. 3 (2019): 1441-1451. https://doi.org/10.3892/ijmm.2019.4070