Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April-2019 Volume 43 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2019 Volume 43 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes

  • Authors:
    • Wen‑Fan Gao
    • Ya‑Yun Xu
    • Jin‑Fang Ge
    • Fei‑Hu Chen
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Anhui Mental Health Center, Hefei, Anhui 230000, P.R. China, School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1778-1788
    |
    Published online on: January 31, 2019
       https://doi.org/10.3892/ijmm.2019.4085
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Acid‑sensing ion channel 1a (ASIC1a), member of the degenerin/epithelial sodium channel protein superfamily, serves a critical role in various physiological and pathological processes. The aim of the present study was to examine the role of ASIC1a in the autophagy of rat articular chondrocytes. Autophagy was induced by acidic stimulation in rat articular chondrocytes and the extent of autophagy was evaluated via the expression levels of microtubule‑associated protein 1 light chain 3II, Beclin1 and uncoordinated‑51 like kinase1. Suppression of ASIC1a was achieved using small interfering RNA technology and/or inhibitor psalmotoxin‑1. The expression levels of autophagy markers were measured by western blot analysis and reverse transcription‑quantitative polymerase chain reaction methods. Intracellular calcium ([Ca2+]i) was analyzed using a Ca2+‑imaging method. Additionally, protein expression levels of the Ca2+/calmodulin‑dependent protein kinase kinase β (CaMKKβ)/5'‑monophosphate‑activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway were measured by western blot analysis. The results showed that autophagy was increased in a pH‑ and time‑dependent manner with exposure to an acidic environment. In addition, silencing ASIC1a significantly decreased the expression levels of autophagy makers, accompanied by abrogation of the acid‑induced [Ca2+]i increase. Furthermore, silencing of ASIC1a downregulated the levels of CaMKKβ/β‑actin and phosphorylated (p‑) AMPK/AMPK, and upregulated the levels of p‑mTOR/mTOR. These results indicated that ASIC1a is a potent regulator of autophagy in chondrocytes, which may be associated with decreased Ca2+ influx and the CaMKKβ/AMPK/mTOR pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Scott DL, Wolfe F and Huizinga TW: Rheumatoid arthritis. Lancet. 376:1094–1108. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Malemud CJ: Chondrocyte apoptosis in rheumatoid arthritis: Is preventive therapy possible? Immunotherapy (Los Angel). 1:1022015.

3 

Chang J, Wang W, Zhang H, Hu Y, Wang M and Yin Z: The dual role of autophagy in chondrocyte responses in the pathogenesis of articular cartilage degeneration in osteoarthritis. Int J Mol Med. 32:1311–1318. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Waldmann R, Champigny G, Bassilana F, Heurteaux C and Lazdunski M: A proton-gated cation channel involved in acid-sensing. Nature. 386:173–177. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Chen CC, England S, Akopian AN and Wood JN: A sensory neuron-specific, proton-gated ion channel. Proc Natl Acad Sci USA. 95:10240–10245. 1998. View Article : Google Scholar : PubMed/NCBI

6 

Lingueglia E, de Weille JR, Bassilana F, Heurteaux C, Sakai H, Waldmann R and Lazdunski M: A modulatory subunit of acid sensing ion channels in brain and dorsal root ganglion cells. J Biol Chem. 272:29778–29783. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Xiong ZG, Chu XP and Simon RP: Ca2+ -permeable acid-sensing ion channels and ischemic brain injury. J Membr Biol. 209:59–68. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Hu W, Chen FH, Yuan FL, Zhang TY, Wu FR, Rong C, Jiang S, Tang J, Zhang CC and Lin MY: Blockade of acid-sensing ion channels protects articular chondrocytes from acid-induced apoptotic injury. Inflamm Res. 61:327–335. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Rong C, Chen FH, Jiang S, Hu W, Wu FR, Chen TY and Yuan FL: Inhibition of acid-sensing ion channels by amiloride protects rat articular chondrocytes from acid-induced apoptosis via a mitochondrial-mediated pathway. Cell Biol Int. 36:635–641. 2012. View Article : Google Scholar

10 

Yuan FL, Chen FH, Lu WG, Li X, Li JP, Li CW, Xu RS, Wu FR, Hu W and Zhang TY: Inhibition of acid-sensing ion channels in articular chondrocytes by amiloride attenuates articular cartilage destruction in rats with adjuvant arthritis. Inflamm Res. 59:939–947. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Martínez-Borra J and López-Larrea C: Autophagy and self-defense. Adv Exp Med Biol. 738:169–184. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Galluzzi L, Morselli E, Vicencio JM, Kepp O, Joza N, Tajeddine N and Kroemer G: Life, death and burial: Multifaceted impact of autophagy. Biochem Soc Trans. 36:786–790. 2008. View Article : Google Scholar : PubMed/NCBI

13 

He C, Zhu H, Li H, Zou MH and Xie Z: Dissociation of Bcl-2-Beclin1 complex by activated AMPK enhances cardiac autophagy and protects against cardiomyocyte apoptosis in diabetes. Diabetes. 62:1270–1281. 2013. View Article : Google Scholar :

14 

Michaud M, Martins I, Sukkurwala AQ, Adjemian S, Ma Y, Pellegatti P, Shen S, Kepp O, Scoazec M, Mignot G, et al: Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science. 334:1573–1577. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Rubinsztein DC: The roles of intracellular protein-degradation pathways in neurodegeneration. Nature. 443:780–786. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Vural A and Kehrl JH: Autophagy in macrophages: Impacting inflammation and bacterial infection. Scientifica (Cairo). 2014:8254632014.

17 

Zhou XJ and Zhang H: Autophagy in immunity: Implications in etiology of autoimmune/autoinflammatory diseases. Autophagy. 8:1286–1299. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Wojtkowiak JW and Gillies RJ: Autophagy on acid. Autophagy. 8:1688–1689. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Caramés B, Hasegawa A, Taniguchi N, Miyaki S, Blanco FJ and Lotz M: Autophagy activation by rapamycin reduces severity of experimental osteoarthritis. Ann Rheum Dis. 71:575–581. 2012. View Article : Google Scholar :

20 

Ureshino RP, Rocha KK, Lopes GS, Bincoletto C and Smaili SS: Calcium signaling alterations, oxidative stress, and autophagy in aging. Antioxid Redox Signal. 21:123–137. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Jung CH, Ro SH, Cao J, Otto NM and Kim DH: mTOR regulation of autophagy. FEBS Lett. 584:1287–1295. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Seldin MM, Lei X, Tan SY, Stanson KP, Wei Z and Wong GW: Skeletal muscle-derived myonectin activates the mammalian target of rapamycin (mTOR) pathway to suppress autophagy in liver. J Biol Chem. 288:36073–36082. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Wu L, Feng Z, Cui S, Hou K, Tang L, Zhou J, Cai G, Xie Y, Hong Q, Fu B and Chen X: Rapamycin upregulates autophagy by inhibiting the mTOR-ULK1 pathway, resulting in reduced podocyte injury. PLoS One. 8:e637992013. View Article : Google Scholar : PubMed/NCBI

24 

Sarkar S, Ravikumar B, Floto RA and Rubinsztein DC: Rapamycin and mTOR-independent autophagy inducers ameliorate toxicity of polyglutamine-expanded huntingtin and related proteinopathies. Cell Death Differ. 16:46–56. 2009. View Article : Google Scholar

25 

Høyer-Hansen M, Bastholm L, Szyniarowski P, Campanella M, Szabadkai G, Farkas T, Bianchi K, Fehrenbacher N, Elling F, Rizzuto R, et al: Control of macroautophagy by calcium, calmodulin-dependent kinase kinase-beta, and Bcl-2. Mol Cell. 25:193–205. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Bohensky J, Leshinsky S, Srinivas V and Shapiro IM: Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression. Pediatr Nephrol. 25:633–642. 2010. View Article : Google Scholar :

27 

Srinivas V, Bohensky J and Shapiro IM: Autophagy: A new phase in the maturation of growth plate chondrocytes is regulated by HIF, mTOR and AMP kinase. Cells Tissues Organs. 189:88–92. 2009. View Article : Google Scholar :

28 

Yuan FL, Chen FH, Lu WG, Li X, Wu FR, Li JP, Li CW, Wang Y, Zhang TY and Hu W: Acid-sensing ion channel 1a mediates acid-induced increases in intracellular calcium in rat articular chondrocytes. Mol Cell Biochem. 340:153–159. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Xiong ZG, Zhu XM, Chu XP, Minami M, Hey J, Wei WL, MacDonald JF, Wemmie JA, Price MP, Welsh MJ and Simon RP: Neuroprotection in ischemia: Blocking calcium-permeable acid-sensing ion channels. Cell. 118:687–698. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T). method Methods. 25:402–408. 2001. View Article : Google Scholar

31 

Mathy-Hartert M, Martin G, Devel P, Deby-Dupont G, Pujol JP, Reginster JY and Henrotin Y: Reactive oxygen species down-regulate the expression of pro- inflammatory genes by human chondrocytes. Inflamm Res. 52:111–118. 2003. View Article : Google Scholar : PubMed/NCBI

32 

Razaq S, Wilkins RJ and Urban JP: The effect of extracellular pH on matrix turnover by cells of the bovine nucleus pulposus. Eur Spine J. 12:341–349. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Mizushima N, Levine B, Cuervo AM and Klionsky DJ: Autophagy fights disease through cellular self-digestion. Nature. 451:1069–1075. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Mariño G, Madeo F and Kroemer G: Autophagy for tissue homeostasis and neuroprotection. Curr Opin Cell Biol. 23:198–206. 2011. View Article : Google Scholar

35 

Levine B and Kroemer G: Autophagy in the pathogenesis of disease. Cell. 132:27–42. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Caramés B, Taniguchi N, Seino D, Blanco FJ, D’Lima D and Lotz M: Mechanical injury suppresses autophagy regulators and pharmacologic activation of autophagy results in chondroprotection. Arthritis Rheum. 64:1182–1192. 2012. View Article : Google Scholar

37 

Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y and Yoshimori T: LC3, a mammalian homologue of yeast Apgp, is localized in autophago-some membranes after processing. EMBO J. 19:5720–5728. 2000. View Article : Google Scholar : PubMed/NCBI

38 

Miki Y, Tanji K, Mori F, Utsumi J, Sasaki H, Kakita A, Takahashi H and Wakabayashi K: Alteration of upstream autophagy-related proteins (ULK1, ULK2, Beclin1, VPS34 and AMBRA1) in lewy body disease. Brain Pathol. 26:359–370. 2016. View Article : Google Scholar

39 

Liu W, Shang G, Yang S, Huang J, Xue X, Lin Y, Zheng Y, Wang X, Wang L, Lin R, et al: Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway. Int J Mol Med. 37:309–318. 2016. View Article : Google Scholar :

40 

Pandey AK, Hazari PP, Patnaik R and Mishra AK: The role of ASIC1a in neuroprotection elicited by quercetin in focal cerebral ischemia. Brain Res. 1383:289–299. 2011. View Article : Google Scholar : PubMed/NCBI

41 

Weng XC, Zheng JQ, Li J and Xiao WB: Underlying mechanism of ASIC1a involved in acidosis-induced cytotoxicity in rat C6 glioma cells. Acta Pharmacol Sin. 28:1731–1736. 2007. View Article : Google Scholar : PubMed/NCBI

42 

Khan MJ, Rizwan Alam M, Waldeck-Weiermair M, Karsten F, Groschner L, Riederer M, Hallström S, Rockenfeller P, Konya V, Heinemann A, et al: Inhibition of autophagy rescues palmitic acid-induced necroptosis of endothelial cells. J Biol Chem. 287:21110–21120. 2012. View Article : Google Scholar : PubMed/NCBI

43 

Wang SH, Shih YL, Ko WC, Wei YH and Shih CM: Cadmium-induced autophagy and apoptosis are mediated by a calcium signaling pathway. Cell Mol Life Sci. 65:3640–3652. 2008. View Article : Google Scholar : PubMed/NCBI

44 

Williams JA, Hou Y, Ni HM and Ding WX: Role of intracellular calcium in proteasome inhibitor-induced endoplasmic reticulum stress, autophagy, and cell death. Pharm Res. 30:2279–2289. 2013. View Article : Google Scholar : PubMed/NCBI

45 

Khan MT and Joseph SK: Role of inositol trisphosphate receptors in autophagy in DT40 cells. J Biol Chem. 285:16912–16920. 2010. View Article : Google Scholar : PubMed/NCBI

46 

Decuypere JP, Bultynck G and Parys JB: A dual role for Ca(2+) in autophagy regulation. Cell calcium. 50:242–250. 2011. View Article : Google Scholar : PubMed/NCBI

47 

Furuta A, Tanaka M, Omata W, Nagasawa M, Kojima I and Shibata H: Microtubule disruption with BAPTA and dimethyl BAPTA by a calcium chelation-independent mechanism in 3T3-L1 adipocytes. Endocr J. 56:235–243. 2009. View Article : Google Scholar

48 

Pfisterer SG, Mauthe M, Codogno P and Proikas-Cezanne T: Ca2+/calmodulin-dependent kinase (CaMK) signaling via CaMKI and AMP-activated protein kinase contributes to the regulation of WIPI-1 at the onset of autophagy. Mol Pharmacol. 80:1066–1075. 2011. View Article : Google Scholar : PubMed/NCBI

49 

Khan NM, Ansari MY and Haqqi TM: Sucrose, but not glucose, blocks IL1- beta-induced inflammatory response in human chondrocytes by inducing autophagy via AKT/mTOR pathway. J Cell Biochem. 118:629–639. 2017. View Article : Google Scholar

50 

Taneike M, Nishida K, Omiya S, Zarrinpashneh E, Misaka T, Kitazume-Taneike R, Austin R, Takaoka M, Yamaguchi O, Gambello MJ, et al: mTOR hyperactivation by ablation of tuberous sclerosis complex 2 in the mouse heart induces cardiac dysfunction with the increased number of small mitochondria mediated through the down-regulation of autophagy. PLoS One. 11:e01526282016. View Article : Google Scholar : PubMed/NCBI

51 

Hirata Y and Oku Y: TRP channels are involved in mediating hypercapnic Ca2+ responses in rat glia-rich medullary cultures independent of extracellular pH. Cell Calcium. 48:124–132. 2010. View Article : Google Scholar : PubMed/NCBI

52 

Takahashi K, Yokota M and Ohta T: Molecular mechanism of 2- APB- induced Ca(2)(+) influx in external acidification in PC12. Exp Cell Res. 323:337–345. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gao WF, Xu YY, Ge JF and Chen FH: Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes. Int J Mol Med 43: 1778-1788, 2019.
APA
Gao, W., Xu, Y., Ge, J., & Chen, F. (2019). Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes. International Journal of Molecular Medicine, 43, 1778-1788. https://doi.org/10.3892/ijmm.2019.4085
MLA
Gao, W., Xu, Y., Ge, J., Chen, F."Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes". International Journal of Molecular Medicine 43.4 (2019): 1778-1788.
Chicago
Gao, W., Xu, Y., Ge, J., Chen, F."Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes". International Journal of Molecular Medicine 43, no. 4 (2019): 1778-1788. https://doi.org/10.3892/ijmm.2019.4085
Copy and paste a formatted citation
x
Spandidos Publications style
Gao WF, Xu YY, Ge JF and Chen FH: Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes. Int J Mol Med 43: 1778-1788, 2019.
APA
Gao, W., Xu, Y., Ge, J., & Chen, F. (2019). Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes. International Journal of Molecular Medicine, 43, 1778-1788. https://doi.org/10.3892/ijmm.2019.4085
MLA
Gao, W., Xu, Y., Ge, J., Chen, F."Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes". International Journal of Molecular Medicine 43.4 (2019): 1778-1788.
Chicago
Gao, W., Xu, Y., Ge, J., Chen, F."Inhibition of acid‑sensing ion channel 1a attenuates acid‑induced activation of autophagy via a calcium signaling pathway in articular chondrocytes". International Journal of Molecular Medicine 43, no. 4 (2019): 1778-1788. https://doi.org/10.3892/ijmm.2019.4085
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team