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LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins

  • Authors:
    • Rabab Asghar Abdulwahab
    • Ayodele Alaiya
    • Zakia Shinwari
    • Abdul Ameer A. Allaith
    • Hayder A. Giha
  • View Affiliations / Copyright

    Affiliations: Integrated Science Division, College of Health Sciences, University of Bahrain, Manama 32038, Kingdom of Bahrain, Proteomics Unit, Stem Cell and Tissue Re‑Engineering Program, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia, Department of Biology College of Science, University of Bahrain, Zallaq 32038, Kingdom of Bahrain, Department of Medical Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, Manama 26671, Kingdom of Bahrain
    Copyright: © Abdulwahab et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2118-2132
    |
    Published online on: March 8, 2019
       https://doi.org/10.3892/ijmm.2019.4127
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Abstract

Type 2 diabetes mellitus (T2DM) is a disease associated with a number of metabolic disturbances, including protein metabolism. In the present study, blood samples were obtained from Bahraini subjects, including 6 patients with T2DM and 6 age‑ and sex‑matched, non‑diabetic, healthy controls. Depleted and non‑depleted sera were prepared from the collected blood, and the global protein expression changes were evaluated by liquid chromatography tandem mass spectrometry. Only significantly and markedly differentially‑expressed proteins (P<0.05, analysis of variance; maximum fold change ≥1.5) were considered as candidate proteins for informatics analysis. Accordingly, a total of 62 proteins were identified to be differentially expressed in T2DM, compared with control subjects, and they were grouped functionally into 16 classes of proteins. The largest class was that of the immune‑associated proteins. Additionally, ~25 of these proteins (40%) had previously been associated with DM; however, the association of the other 37 proteins with T2DM was a novel observation. The majority of the identified proteins were upregulated in T2DM. The identified proteins could be involved in the pathogenesis of the disease or serve as disease biomarkers. Further validation of the identified proteins in a large study cohort is required, in order to fully access their potential clinical usefulness.
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Copy and paste a formatted citation
Spandidos Publications style
Abdulwahab RA, Alaiya A, Shinwari Z, Allaith AA and Giha HA: LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins . Int J Mol Med 43: 2118-2132, 2019.
APA
Abdulwahab, R.A., Alaiya, A., Shinwari, Z., Allaith, A.A., & Giha, H.A. (2019). LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins . International Journal of Molecular Medicine, 43, 2118-2132. https://doi.org/10.3892/ijmm.2019.4127
MLA
Abdulwahab, R. A., Alaiya, A., Shinwari, Z., Allaith, A. A., Giha, H. A."LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins ". International Journal of Molecular Medicine 43.5 (2019): 2118-2132.
Chicago
Abdulwahab, R. A., Alaiya, A., Shinwari, Z., Allaith, A. A., Giha, H. A."LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins ". International Journal of Molecular Medicine 43, no. 5 (2019): 2118-2132. https://doi.org/10.3892/ijmm.2019.4127
Copy and paste a formatted citation
x
Spandidos Publications style
Abdulwahab RA, Alaiya A, Shinwari Z, Allaith AA and Giha HA: LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins . Int J Mol Med 43: 2118-2132, 2019.
APA
Abdulwahab, R.A., Alaiya, A., Shinwari, Z., Allaith, A.A., & Giha, H.A. (2019). LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins . International Journal of Molecular Medicine, 43, 2118-2132. https://doi.org/10.3892/ijmm.2019.4127
MLA
Abdulwahab, R. A., Alaiya, A., Shinwari, Z., Allaith, A. A., Giha, H. A."LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins ". International Journal of Molecular Medicine 43.5 (2019): 2118-2132.
Chicago
Abdulwahab, R. A., Alaiya, A., Shinwari, Z., Allaith, A. A., Giha, H. A."LC‑MS/MS proteomic analysis revealed novel associations of 37 proteins with T2DM and notable upregulation of immunoglobulins ". International Journal of Molecular Medicine 43, no. 5 (2019): 2118-2132. https://doi.org/10.3892/ijmm.2019.4127
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