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Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway

  • Authors:
    • Min Wang
    • Ying Liu
    • Rui‑Le Pan
    • Rui‑Ying Wang
    • Shi‑Lan Ding
    • Wan‑Rui Dong
    • Gui‑Bo Sun
    • Jing‑Xue Ye
    • Xiao‑Bo Sun
  • View Affiliations / Copyright

    Affiliations: Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine (Natural Medicine) and Translational Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, P.R. China, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China, Harbin University of Commerce, Harbin, Heilongjiang 150076, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2133-2143
    |
    Published online on: March 12, 2019
       https://doi.org/10.3892/ijmm.2019.4131
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Abstract

Myrica rubra is well known for its delicious taste and high nutritional value. The present study investigated the potential protective effects and mechanisms of M. rubra flavonoids (MRF) extract on isoproterenol (ISO)‑induced myocardial injury in rats and hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes. An in vivo study revealed that MRF decreased serum cardiac enzyme levels, ameliorated pathological heart alterations and increased the antioxidant potential. The in vitro investigation demonstrated that MRF inhibited cell death, reactive oxygen species (ROS) accumulation, mitochondrial membrane depolarization, apoptosis rate and caspase‑3 activation and enhanced the Bcl‑2/Bax ratio during H/R injury. These effects were accompanied by the phosphorylation of protein kinase B (Akt) and glycogen synthase kinase (GSK)‑3β. Further mechanism studies demonstrated that LY294002, a specific inhibitor of phosphoinositide 3‑kinase (PI3K), abolished the MRF‑mediated cardioprotection against H/R‑induced apoptosis and ROS overproduction. Collectively, these results suggested that MRF exerts cardioprotective effects by attenuating oxidative damage and cardiomyocyte apoptosis most likely via a PI3K/Akt/GSK3β‑dependent mechanism.
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Copy and paste a formatted citation
Spandidos Publications style
Wang M, Liu Y, Pan RL, Wang RY, Ding SL, Dong WR, Sun GB, Ye JX and Sun XB: Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway. Int J Mol Med 43: 2133-2143, 2019.
APA
Wang, M., Liu, Y., Pan, R., Wang, R., Ding, S., Dong, W. ... Sun, X. (2019). Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway. International Journal of Molecular Medicine, 43, 2133-2143. https://doi.org/10.3892/ijmm.2019.4131
MLA
Wang, M., Liu, Y., Pan, R., Wang, R., Ding, S., Dong, W., Sun, G., Ye, J., Sun, X."Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway". International Journal of Molecular Medicine 43.5 (2019): 2133-2143.
Chicago
Wang, M., Liu, Y., Pan, R., Wang, R., Ding, S., Dong, W., Sun, G., Ye, J., Sun, X."Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway". International Journal of Molecular Medicine 43, no. 5 (2019): 2133-2143. https://doi.org/10.3892/ijmm.2019.4131
Copy and paste a formatted citation
x
Spandidos Publications style
Wang M, Liu Y, Pan RL, Wang RY, Ding SL, Dong WR, Sun GB, Ye JX and Sun XB: Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway. Int J Mol Med 43: 2133-2143, 2019.
APA
Wang, M., Liu, Y., Pan, R., Wang, R., Ding, S., Dong, W. ... Sun, X. (2019). Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway. International Journal of Molecular Medicine, 43, 2133-2143. https://doi.org/10.3892/ijmm.2019.4131
MLA
Wang, M., Liu, Y., Pan, R., Wang, R., Ding, S., Dong, W., Sun, G., Ye, J., Sun, X."Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway". International Journal of Molecular Medicine 43.5 (2019): 2133-2143.
Chicago
Wang, M., Liu, Y., Pan, R., Wang, R., Ding, S., Dong, W., Sun, G., Ye, J., Sun, X."Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3β pathway". International Journal of Molecular Medicine 43, no. 5 (2019): 2133-2143. https://doi.org/10.3892/ijmm.2019.4131
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