Open Access

Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription

  • Authors:
    • Susumu Tanaka
    • Yoshiko Honda
    • Shizuka Takaku
    • Taro Koike
    • Souichi Oe
    • Yukie Hirahara
    • Takashi Yoshida
    • Nae Takizawa
    • Yasuharu Takamori
    • Kiyoshi Kurokawa
    • Tohru Kodama
    • Hisao Yamada
  • View Affiliations

  • Published online on: March 20, 2019     https://doi.org/10.3892/ijmm.2019.4143
  • Pages: 2164-2176
  • Copyright: © Tanaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The hypocretin/orexin neuropeptide system coordinates the regulation of various physiological processes. Our previous study reported that a reduction in the expression of pleomorphic adenoma gene‑like 1 (Plagl1), which encodes a C2H2 zinc‑finger transcription factor, occurs in hypocretin neuron‑ablated transgenic mice, suggesting that PLAGL1 is co‑expressed in hypocretin neurons and regulates hypocretin transcription. The present study examined whether canonical prepro‑hypocretin transcription is functionally modulated by PLAGL1. Double immunostaining indicated that the majority of hypocretin neurons were positive for PLAGL1 immunoreactivity in the nucleus. Notably, PLAGL1 immunoreactivity in hypocretin neurons was altered in response to several conditions affecting hypocretin function. An uneven localization of PLAGL1 was detected in the nuclei of hypocretin neurons following sleep deprivation. Chromatin immunoprecipitation revealed that endogenous PLAGL1 may bind to a putative PLAGL1‑binding site in the proximal region of the hypocretin gene, in the murine hypothalamus. In addition, electroporation of the PLAGL1 expression vector into the fetal hypothalamus promoted hypothalamic hypocretin transcription. These results suggested that PLAGL1 may regulate hypothalamic hypocretin transcription.
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May-2019
Volume 43 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tanaka S, Honda Y, Takaku S, Koike T, Oe S, Hirahara Y, Yoshida T, Takizawa N, Takamori Y, Kurokawa K, Kurokawa K, et al: Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription. Int J Mol Med 43: 2164-2176, 2019
APA
Tanaka, S., Honda, Y., Takaku, S., Koike, T., Oe, S., Hirahara, Y. ... Yamada, H. (2019). Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription. International Journal of Molecular Medicine, 43, 2164-2176. https://doi.org/10.3892/ijmm.2019.4143
MLA
Tanaka, S., Honda, Y., Takaku, S., Koike, T., Oe, S., Hirahara, Y., Yoshida, T., Takizawa, N., Takamori, Y., Kurokawa, K., Kodama, T., Yamada, H."Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription". International Journal of Molecular Medicine 43.5 (2019): 2164-2176.
Chicago
Tanaka, S., Honda, Y., Takaku, S., Koike, T., Oe, S., Hirahara, Y., Yoshida, T., Takizawa, N., Takamori, Y., Kurokawa, K., Kodama, T., Yamada, H."Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription". International Journal of Molecular Medicine 43, no. 5 (2019): 2164-2176. https://doi.org/10.3892/ijmm.2019.4143