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Development of finasteride polymer microspheres for systemic application in androgenic alopecia

  • Authors:
    • Ju Hee Kim
    • Jungtae Na
    • Dong‑Ho Bak
    • Byung Chul Lee
    • Esther Lee
    • Mi Ji Choi
    • Choong Ho Ryu
    • Sangno Lee
    • Seog‑Kyun Mun
    • Byung Cheol Park
    • Beom Joon Kim
    • Hyun‑Shik Lee
  • View Affiliations / Copyright

    Affiliations: KNU‑Center for Nonlinear Dynamics, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Science Kyungpook National University, Daegu 41566, Republic of Korea, Department of Dermatology, College of Medicine, Chung‑Ang University, Seoul 06973, Republic of Korea, Headquarter, Inventage Lab, Inc., Seongnam, Gyeonggi 13403, Republic of Korea, Department of Otorhinolaryngology‑Head and Neck Surgery, College of Medicine, Chung‑Ang University, Seoul 06973, Republic of Korea , Department of Dermatology, Dankook Medical College, Cheonan, Chungcheongnam 31116, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2409-2419
    |
    Published online on: March 28, 2019
       https://doi.org/10.3892/ijmm.2019.4149
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Abstract

The use of finasteride for alleviating hair loss has been investigated, and it has been applied as an oral dose medication. However, due to the inconvenience of daily drug administration over long period of time, novel controllable finasteride delivery has been actively investigated. As a novel method of finasteride delivery, the development of finasteride‑loaded microspheres for subcutaneous administration is becoming increasingly pharmaceutically important. Therefore, the present study aimed to use finasteride‑loaded microspheres in a controlled manner in an attempt to overcome the limitations of the oral administration of finasteride and to cause fewer adverse effects. Finasteride‑loaded microspheres containing poly(lactic‑co‑glycolic acid) and finasteride at a ratio of 4:1 were prepared, and a testosterone‑induced androgenic alopecia mouse model was used. Following observation for 10 weeks, the percentage hair growth was 86.7% (total hair growth 60%, partial hair growth 26.7%) in the orally‑applied finasteride‑treated group as a positive control, and 93.3% (total hair growth 60%, partial hair growth 33.3%) in the finasteride‑loaded microspheres‑treated group. Serum dihydrotestosterone levels began to decrease at week 6 in the orally‑applied finasteride‑ and finasteride‑loaded microsphere‑treated groups. In addition, the finasteride‑loaded microspheres‑treated group exhibited similar follicular number, follicular length, anagen/telogen ratio and hair bulb diameter values to those of the orally‑applied finasteride‑treated group. Furthermore, the finasteride‑loaded microspheres increased the activities of phosphoinositide 3‑kinase/protein kinase B and Wnt/β‑catenin in relation to hair follicle cell growth signaling in mouse skin, and suppressed the apoptosis of hair follicle cells by reducing the expression of transforming growth factor‑β2 and caspase‑3, which are indicators of apoptosis. In conclusion, the administration of a single injection of finasteride‑loaded microspheres was effective in treating testosterone‑induced alopecia. Furthermore, it led to equivalent hair growth effects when compared with orally‑applied finasteride, thus revealing the possibility of effective treatment via different routes of administration.
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Copy and paste a formatted citation
Spandidos Publications style
Kim JH, Na J, Bak DH, Lee BC, Lee E, Choi MJ, Ryu CH, Lee S, Mun SK, Park BC, Park BC, et al: Development of finasteride polymer microspheres for systemic application in androgenic alopecia. Int J Mol Med 43: 2409-2419, 2019.
APA
Kim, J.H., Na, J., Bak, D., Lee, B.C., Lee, E., Choi, M.J. ... Lee, H. (2019). Development of finasteride polymer microspheres for systemic application in androgenic alopecia. International Journal of Molecular Medicine, 43, 2409-2419. https://doi.org/10.3892/ijmm.2019.4149
MLA
Kim, J. H., Na, J., Bak, D., Lee, B. C., Lee, E., Choi, M. J., Ryu, C. H., Lee, S., Mun, S., Park, B. C., Kim, B. J., Lee, H."Development of finasteride polymer microspheres for systemic application in androgenic alopecia". International Journal of Molecular Medicine 43.6 (2019): 2409-2419.
Chicago
Kim, J. H., Na, J., Bak, D., Lee, B. C., Lee, E., Choi, M. J., Ryu, C. H., Lee, S., Mun, S., Park, B. C., Kim, B. J., Lee, H."Development of finasteride polymer microspheres for systemic application in androgenic alopecia". International Journal of Molecular Medicine 43, no. 6 (2019): 2409-2419. https://doi.org/10.3892/ijmm.2019.4149
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JH, Na J, Bak DH, Lee BC, Lee E, Choi MJ, Ryu CH, Lee S, Mun SK, Park BC, Park BC, et al: Development of finasteride polymer microspheres for systemic application in androgenic alopecia. Int J Mol Med 43: 2409-2419, 2019.
APA
Kim, J.H., Na, J., Bak, D., Lee, B.C., Lee, E., Choi, M.J. ... Lee, H. (2019). Development of finasteride polymer microspheres for systemic application in androgenic alopecia. International Journal of Molecular Medicine, 43, 2409-2419. https://doi.org/10.3892/ijmm.2019.4149
MLA
Kim, J. H., Na, J., Bak, D., Lee, B. C., Lee, E., Choi, M. J., Ryu, C. H., Lee, S., Mun, S., Park, B. C., Kim, B. J., Lee, H."Development of finasteride polymer microspheres for systemic application in androgenic alopecia". International Journal of Molecular Medicine 43.6 (2019): 2409-2419.
Chicago
Kim, J. H., Na, J., Bak, D., Lee, B. C., Lee, E., Choi, M. J., Ryu, C. H., Lee, S., Mun, S., Park, B. C., Kim, B. J., Lee, H."Development of finasteride polymer microspheres for systemic application in androgenic alopecia". International Journal of Molecular Medicine 43, no. 6 (2019): 2409-2419. https://doi.org/10.3892/ijmm.2019.4149
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