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Article

Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion

  • Authors:
    • Xiuling Zhang
    • Xianxian Cao
    • Mengqiu Dang
    • Hongxia Wang
    • Buxing Chen
    • Fenghe Du
    • Huihua Li
    • Xiangjun Zeng
    • Caixia Guo
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100070, P.R. China, Department of Physiology and Pathophysiology, Capital Medical University, Beijing 100069, P.R. China, Department of Cardiology, Institute of Cardiovascular Disease, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 2507-2515
    |
    Published online on: March 29, 2019
       https://doi.org/10.3892/ijmm.2019.4152
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Abstract

The current study investigated the role of sRAGE in the production of IFN‑γ in macrophages with I/R treatment. The number of macrophages in myocardial tissues treated with I/R with or without sRAGE was determined via immunohistochemical staining. Proliferative activity of macrophages was analyzed by a 5‑BrdU incorporation assay. Differentiation of macrophages was detected via immunofluorescence staining of iNOS (M1 macrophage marker). IFN‑γ production, due to sRAGE stimulation, in Raw 264.7 macrophages and the NF‑κB signaling pathway were measured using western blotting. A ChIP assay was used to examine the interactions between NF‑κB and the promoter of IFN‑γ. The results showed that the number of macrophages in I/R‑treated myocardial tissues was increased following sRAGE infusion. Proliferation of macrophages was increased significantly in the presence of sRAGE; after I/R treatment, the cells preferred to differentiate into M1 macrophages. IFN‑γ expression in Raw 264.7 macrophages was suppressed by an NF‑κB inhibitor (Bay117082) but enhanced by sRAGE, with or without I/R treatment. Furthermore, sRAGE increased the phosphorylation of IκB, IKK and NF‑κB, as well as the translocation of NF‑κB into the nucleus of Raw 264.7 macrophages, with or without I/R treatment. ChIP results showed that sRAGE promoted NF‑κB binding to the promoter of IFN‑γ in Raw 264.7 macrophages. Therefore, the findings of the present study indicated that sRAGE protected the heart from I/R injuries, which might be mediated by promoting infiltration and the differentiation of macrophages into M1, which would then synthesize and secrete IFN‑γ through activating the NF‑κB signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Cao X, Dang M, Wang H, Chen B, Du F, Li H, Zeng X and Guo C: Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion. Int J Mol Med 43: 2507-2515, 2019.
APA
Zhang, X., Cao, X., Dang, M., Wang, H., Chen, B., Du, F. ... Guo, C. (2019). Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion. International Journal of Molecular Medicine, 43, 2507-2515. https://doi.org/10.3892/ijmm.2019.4152
MLA
Zhang, X., Cao, X., Dang, M., Wang, H., Chen, B., Du, F., Li, H., Zeng, X., Guo, C."Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion". International Journal of Molecular Medicine 43.6 (2019): 2507-2515.
Chicago
Zhang, X., Cao, X., Dang, M., Wang, H., Chen, B., Du, F., Li, H., Zeng, X., Guo, C."Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion". International Journal of Molecular Medicine 43, no. 6 (2019): 2507-2515. https://doi.org/10.3892/ijmm.2019.4152
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Cao X, Dang M, Wang H, Chen B, Du F, Li H, Zeng X and Guo C: Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion. Int J Mol Med 43: 2507-2515, 2019.
APA
Zhang, X., Cao, X., Dang, M., Wang, H., Chen, B., Du, F. ... Guo, C. (2019). Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion. International Journal of Molecular Medicine, 43, 2507-2515. https://doi.org/10.3892/ijmm.2019.4152
MLA
Zhang, X., Cao, X., Dang, M., Wang, H., Chen, B., Du, F., Li, H., Zeng, X., Guo, C."Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion". International Journal of Molecular Medicine 43.6 (2019): 2507-2515.
Chicago
Zhang, X., Cao, X., Dang, M., Wang, H., Chen, B., Du, F., Li, H., Zeng, X., Guo, C."Soluble receptor for advanced glycation end-products enhanced the production of IFN-γ through the NF-κB pathway in macrophages recruited by ischemia/reperfusion". International Journal of Molecular Medicine 43, no. 6 (2019): 2507-2515. https://doi.org/10.3892/ijmm.2019.4152
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