Structural characterization and immune regulation of a novel polysaccharide from Maerkang Lactarius deliciosus Gray

Su,Siyuan; Ding,Xiang; Fu,Lei; Hou,Yiling

doi:10.3892/ijmm.2019.4219

Structural characterization and immune regulation of a novel polysaccharide from Maerkang Lactarius deliciosus Gray

Authors:
- Siyuan Su
- Xiang Ding
- Lei Fu
- Yiling Hou
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Affiliations: Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, P.R. China, College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan 637009, P.R. China
Published online on: May 30, 2019 https://doi.org/10.3892/ijmm.2019.4219
Pages: 713-724

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Abstract

The present study investigated the structural characterization and immune regulation of a novel polysaccharide from Maerkang Lactarius deliciosus Gray. Chemical methods, high performance gel permeation chromatography, fourier transform infrared spectroscopy, nuclear magnetic resonance spectrum and gas chromatography‑mass spectrometry were used to characterize the polysaccharide structure. The immunomodulatory abilities of the Maerkang L. deliciosus Gray polysaccharide (LDG‑M) were also investigated. LDG‑M was primarily composed of β‑D‑glucose and α‑D‑lyxose with the ratio of 2:1. The possible structure of LDG‑M had a backbone of 1,6‑linked‑β‑D‑glucose and 1,4,6‑linked‑β‑D‑glucose, with branches primarily composed of one (1→4)‑linked‑α‑D‑lyxose residue. The immunoregulatory activity results demonstrated that LDG‑M promoted the proliferation and phagocytosis of macrophages, and induced cytokine release. LDG‑M also promoted the proliferation of B cells by affecting the G0/G1, S and G2/M phases. The present study introduced LDG‑M as a valuable source with unique immunoregulatory properties.

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1	Wang M, Yang XB, Zhao JW, Lu CJ and Zhu W: Structural characterization and macrophage immunomodulatory activity of a novel polysaccharide from Smilax glabra Roxb. Carbohydr Polym. 156:390–402. 2017. View Article : Google Scholar
2	Yu Y, Shen MY, Song QQ and Xie J: Biological activities and pharmaceutical applications of polysaccharide from natural resources: A review. Carbohydr Polym. 183:91–101. 2018. View Article : Google Scholar : PubMed/NCBI
3	Cho CW, Han CJ, Rhee YK, Lee YC, Shin KS, Shin JS, Lee KT and Hong HD: Cheonggukjang polysaccharides enhance immune activities and prevent cyclophosphamide-induced immunosuppression. Int J Biol Macromol. 72:519–525. 2015. View Article : Google Scholar
4	Li N, Li L, Fang JC, Wong JH, Ng TB, Jiang Y, Wang CR, Zhang NY, Wen TY, Qu LY, et al: Isolation and identification of a novel polysaccharide-peptide complex with antioxidant, anti-proliferative and hypoglycemic activities from the abalone mushroom. Biosci Rep. 32:221–228. 2012. View Article : Google Scholar
5	Li LJ, Li MY, Li YT, Feng JJ, Hao FQ and Lun Z: Adjuvant activity of Sargassum pallidum polysaccharides against combined newcastle disease, infectious bronchitis and avian influenza inactivated vaccines. Mar Drugs. 10:2648–2660. 2012. View Article : Google Scholar :
6	Huang M, Wang FQ, Zhou XH, Yang H and Wang Y: Hypoglycemic and hypolipidemic properties of polysaccharides from Enterobacter cloacae Z0206 in KKAy mice. Carbohydr Polym. 117:91–98. 2015. View Article : Google Scholar
7	Chen XD, Wu QX, Zhao J, Su T, Lu YM, Zhang WN, Wang Y and Chen Y: Immunomodulatory effect of a polysaccharide fraction on RAW 264.7 macrophages extracted from the wild Lactarius deliciosus. Int J Biol Macromol. 128:732–739. 2019. View Article : Google Scholar : PubMed/NCBI
8	Ding X, Hou YL and Hou WR: Structure feature and antitumor activity of a novel polysaccharide isolated from Lactarius deliciosus Gray. Carbohydr Polym. 89:397–402. 2012. View Article : Google Scholar : PubMed/NCBI
9	Hou Y, Ding X, Hou W, Song B, Wang T, Wang F and Zhong J: Immunostimulant activity of a novel polysaccharide isolated from Lactarius deliciosus (L. ex Fr) Gray. Indian J Pharm Sci. 75:393–399. 2013. View Article : Google Scholar : PubMed/NCBI
10	Hou YL, Wang M, Zhao DQ, Liu L, Ding X and Hou W: Effect on macrophage proliferation of a novel polysaccharide from Lactarius deliciosus (L. ex Fr) Gray. Oncol Lett. 17:2507–2515. 2019.PubMed/NCBI
11	Hou YL, Liu L, Ding X, Zhao D and Hou W: Structure elucidation, proliferation effect on macrophage and its mechanism of a new heteropolysaccharide from Lactarius deliciosus Gray. Carbohydr Polym. 152:648–657. 2016. View Article : Google Scholar : PubMed/NCBI
12	Zhu Y, Ding X, Wang M, Hou Y, Hou W and Yue C: Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea. Mol Med Rep. 14:3947–3954. 2016. View Article : Google Scholar : PubMed/NCBI
13	Ding X, Hou Y, Zhu Y, Wang P, Fu L, Zhu H, Zhang N, Qin H, Qu W, Wang F and Hou W: Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray. Oncol Rep. 33:3162–3170. 2015. View Article : Google Scholar : PubMed/NCBI
14	Ding X, Hou Y, Hou W, Zhu Y, Fu L and Zhu H: Structure elucidation and anti-tumor activities of water-soluble oligosaccharides from Lactarius deliciosus (L. ex Fr) Gray. Pharmacogn Mag. 11:716–723. 2015. View Article : Google Scholar : PubMed/NCBI
15	Ding X, Feng S, Cao M, Li MT, Tang J, Guo CX, Zhang J, Sun Q, Yang ZR and Zhao J: Structure characterization of polysaccharide isolated from the fruiting bodies of Tricholoma matsutake. Carbohydr Polym. 81:942–947. 2010. View Article : Google Scholar
16	Hou Y, Ding X, Hou W, Zhong J, Zhu H, Ma B, Xu T and Li J: Anti-microorganism, anti-tumor, and immune activities of a novel polysaccharide isolated from Tricholoma matsutake. Pharmacogn Mag. 9:244–249. 2013. View Article : Google Scholar : PubMed/NCBI
17	Wang M, Jiang C, Ma L, Zhang Z, Cao L, Liu J and Zeng X: Preparation, preliminary characterization and immunostimulatory activity of polysaccharide fractions from the peduncles of Hovenia dulcis. Food Chem. 138:41–47. 2013. View Article : Google Scholar
18	Zhou X, Wang H, Wang B, Fu L, Yuan M, Liu J, Zhou L and Ding C: Characterization and antioxidant activities of polysaccharides from the leaves of Lilium lancifolium Thunb. Int J Biol Macromol. 92:148–155. 2016. View Article : Google Scholar : PubMed/NCBI
19	Liao W, Luo Z, Liu D, Ning Z, Yang J and Ren J: Structure characterization of a novel polysaccharide from Dictyophora indusiata and its macrophage immunomodulatory activities. J Agric Food Chem. 63:535–544. 2015. View Article : Google Scholar
20	Jing Y, Huang L, Lv W, Tong H, Song L, Hu X and Yu R: Structural characterization of a novel polysaccharide from pulp tissues of Litchi chinensis and its immunomodulatory activity. J Agric Food Chem. 62:902–911. 2014. View Article : Google Scholar
21	Lee JS, Synytsya A, Kim HB, Choi DJ, Lee S, Lee J, Kim WJ, Jang S and Park YI: Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.). Int Immunopharmacol. 17:858–866. 2013. View Article : Google Scholar : PubMed/NCBI
22	Kamnev AA, Tugarova AV, Dyatlova YA, Tarantilis PA, Grigoryeva OP, Fainleib AM and De Luca S: Methodological effects in Fourier transform infrared (FTIR) spectroscopy: Implications for structural analyses of biomacromolecular samples. Spectrochim Acta A Mol Biomol Spectrosc. 193:558–564. 2018. View Article : Google Scholar : PubMed/NCBI
23	Tong H, Mao D, Zhai M, Zhang Z, Sun G and Jiang G: Macrophage activation induced by the polysaccharides isolated from the roots of Sanguisorba officinalis. Pharm Biol. 53:1511–1515. 2015. View Article : Google Scholar : PubMed/NCBI
24	van Leeuwen SS, Kuipers BJH, Dijkhuizen L and Kamerling JP: Development of a (1)H NMR structural-reporter-group concept for the analysis of prebiotic galacto-oligosaccharides of the [β-d-Galp-(1→x)]n-d-Glcp type. Carbohyd Res. 400:54–58. 2014. View Article : Google Scholar
25	Maity P, Nandi AK, Manna DK, Pattanayak M, Sen IK, Bhanja SK, Samanta S, Panda BC, Paloi S, Acharya K and Islam SS: Structural characterization and antioxidant activity of a glucan from Meripilus giganteus. Carbohydr Polym. 157:1237–1245. 2017. View Article : Google Scholar
26	Liu L, Jia J, Zeng G, Zhao Y, Qi X, He C, Guo W, Fan D, Han G and Li Z: Studies on immunoregulatory and anti-tumor activities of a polysaccharide from Salvia miltiorrhiza Bunge. Carbohydr Polym. 92:479–483. 2013. View Article : Google Scholar
27	Zhu H, Ding X, Hou Y, Li Y and Wang M: Structure elucidation and bioactivities of a new polysaccharide from Xiaojin Boletus speciosus Frost. Int J Biol Macromol. 126:697–716. 2019. View Article : Google Scholar
28	Haneef J, Parvathy M, Thankayyan RSK, Sithul H and Sreeharshan S: Bax translocation mediated mitochondrial apoptosis and caspase dependent photosensitizing effect of ficus religiosa on cancer cells. PLoS One. 7:e400552012. View Article : Google Scholar : PubMed/NCBI
29	Zhang W, Huang J, Wang W, Li Q, Chen Y, Feng W, Zheng D, Zhao T, Mao G, Yang L and Wu X: Extraction, purification, characterization and antioxidant activities of polysaccharides from Cistanche tubulosa. Int J Biol Macromol. 93:448–458. 2016. View Article : Google Scholar : PubMed/NCBI
30	Liu J, Wen XY, Zhang XQ, Pu HM, Kan J and Jin CH: Extraction, characterization and in vitro antioxidant activity of polysaccharides from black soybean. Int J Biol Macromol. 72:1182–1190. 2015. View Article : Google Scholar
31	Liu L, Ding X, Hou Y, Song B, Zhao D and Hou W: Structural characterization and immunological activity of a novel hetero-polysaccharide from Lactikporus supharells (Fr) murr. Latin Am J Pharm. 36:2386–2396. 2017.
32	Gomez Perez M, Fourcade L, Mateescu MA and Paquin J: Neutral Red versus MTT assay of cell viability in the presence of copper compounds. Anal Biochem. 535:43–46. 2017. View Article : Google Scholar : PubMed/NCBI
33	Dulger B, Yilmaz F and Gucin F: Antimicrobial activity of some Lactarius species. Pharm Biol. 40:304–306. 2002. View Article : Google Scholar
34	Fernandes Â, Antonio AL, Barreira J, Botelho L, Oliveira MB, Martins A and Ferreira I: Effects of gamma irradiation on the chemical composition and antioxidant activity of Lactarius deliciosus L. wild edible mushroom. Food Bioproc Technol. 6:2895–2903. 2013. View Article : Google Scholar
35	Dwek RA: Glycobiology: Toward understanding the function of sugars. Chem Rev. 96:683–720. 1996. View Article : Google Scholar : PubMed/NCBI
36	Fan S, Zhang J, Nie W, Zhou W, Jin L, Chen X and Lu J: Antitumor effects of polysaccharide from Sargassum fusiforme against human hepatocellular carcinoma HepG2 cells. Food Chem Toxicol. 102:53–62. 2017. View Article : Google Scholar : PubMed/NCBI
37	Zong A, Cao H and Wang F: Anticancer polysaccharides from natural resources: A review of recent research. Carbohydr Polym. 90:1395–1410. 2012. View Article : Google Scholar : PubMed/NCBI
38	Xie JH, Liu X, Shen MY, Nie SP, Zhang H, Li C, Gong DM and Xie MY: Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves. Food Chem. 136:1453–1460. 2013. View Article : Google Scholar
39	Li S, Gao A, Dong S, Chen Y, Sun S, Lei Z and Zhang Z: Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta. Int J Biol Macromol. 96:26–34. 2017. View Article : Google Scholar
40	Li QM, Wang JF, Zha XQ, Pan LH, Zhang HL and Luo JP: Structural characterization and immunomodulatory activity of a new polysaccharide from jellyfish. Carbohydr Polym. 159:188–194. 2017. View Article : Google Scholar : PubMed/NCBI
41	Zhang Y, Li S, Wang X, Zhang L and Cheung PCK: Advances in lentinan: Isolation, structure, chain conformation and bioactivities. Food Hydrocoll. 25:196–206. 2011. View Article : Google Scholar
42	Bisen PS, Baghel RK, Sanodiya BS, Thakur GS and Prasad GB: Lentinus edodes: A macrofungus with pharmacological activities. Curr Med Chem. 17:2419–2430. 2010. View Article : Google Scholar : PubMed/NCBI