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Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms

  • Authors:
    • Shan Gao
    • Shiqin Li
    • Qin Li
    • Fuyong Zhang
    • Mengqi Sun
    • Zilin Wan
    • Shurong Wang
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Infectious Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Pharmacy, People's Hospital of Deyang City, Deyang, Sichuan 618000, P.R. China, Department of Pharmacy, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 457-468
    |
    Published online on: May 31, 2019
       https://doi.org/10.3892/ijmm.2019.4227
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Abstract

Salvianolic acid B (Sal B) is a water‑soluble active component of Danshen and has anti‑atherosclerotic effects. The present study aimed to evaluate the cytoprotective effects of Sal B against hydrogen peroxide (H2O2)‑induced oxidative stress damage in human umbilical vein endothelial cells (HUVECs) and investigate the underlying mechanisms. It was revealed that Sal B protected the cells from H2O2‑induced damage, as indicated by MTT results showing enhanced cell viability and by flow cytometric analysis showing reduced apoptosis of cells challenged with H2O2. Furthermore, as an underlying mechanism, the enhancement of autophagy was indicated to be accountable for the decrease in apoptosis, as Sal B caused the upregulation of light chain 3‑Ⅱ and Beclin‑1, and downregulation of p62 under H2O2‑induced oxidative stress. Finally, Sal B increased the phosphorylation of AMP kinase (AMPK) and decreased the phosphorylation of mammalian target of rapamycin (mTOR), but had no effect on the phosphorylation of AKT. In conclusion, the present study revealed that Sal B protects HUVECs from oxidative stress, at least partially by promoting autophagy via activation of the AMPK pathway and downregulation of the mTOR pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Gao S, Li S, Li Q, Zhang F, Sun M, Wan Z and Wang S: Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms. Int J Mol Med 44: 457-468, 2019.
APA
Gao, S., Li, S., Li, Q., Zhang, F., Sun, M., Wan, Z., & Wang, S. (2019). Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms. International Journal of Molecular Medicine, 44, 457-468. https://doi.org/10.3892/ijmm.2019.4227
MLA
Gao, S., Li, S., Li, Q., Zhang, F., Sun, M., Wan, Z., Wang, S."Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms". International Journal of Molecular Medicine 44.2 (2019): 457-468.
Chicago
Gao, S., Li, S., Li, Q., Zhang, F., Sun, M., Wan, Z., Wang, S."Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms". International Journal of Molecular Medicine 44, no. 2 (2019): 457-468. https://doi.org/10.3892/ijmm.2019.4227
Copy and paste a formatted citation
x
Spandidos Publications style
Gao S, Li S, Li Q, Zhang F, Sun M, Wan Z and Wang S: Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms. Int J Mol Med 44: 457-468, 2019.
APA
Gao, S., Li, S., Li, Q., Zhang, F., Sun, M., Wan, Z., & Wang, S. (2019). Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms. International Journal of Molecular Medicine, 44, 457-468. https://doi.org/10.3892/ijmm.2019.4227
MLA
Gao, S., Li, S., Li, Q., Zhang, F., Sun, M., Wan, Z., Wang, S."Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms". International Journal of Molecular Medicine 44.2 (2019): 457-468.
Chicago
Gao, S., Li, S., Li, Q., Zhang, F., Sun, M., Wan, Z., Wang, S."Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms". International Journal of Molecular Medicine 44, no. 2 (2019): 457-468. https://doi.org/10.3892/ijmm.2019.4227
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