Open Access

The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects

  • Authors:
    • Xiaoyan Yuan
    • Lu Han
    • Hai Lin
    • Zeyou Guo
    • Yanling Huang
    • Shasha Li
    • Ting Long
    • Wei Tang
    • Weidong Tian
    • Jie Long
  • View Affiliations

  • Published online on: June 20, 2019     https://doi.org/10.3892/ijmm.2019.4249
  • Pages: 857-870
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue‑engineered bone grafts remains unclear. We previously reported that microRNA (miR)‑26a‑5p inhibited the osteogenic differentiation of adipose‑derived mesenchymal stem cells (ADSCs), and that antimiR‑26a‑5p exerted the opposite effect. In the present study, the role of miR‑26a‑5p‑ and antimiR‑26a‑5p‑modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR‑26a‑5p in bone regeneration in vivo, as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv‑miR‑26a‑5p, Lv‑miR‑NC, Lv‑antimiR‑26a‑5p or Lv‑antimiR‑NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X‑rays, micro‑computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR‑26a‑5p and the lowest in the miR‑26a‑5p treatment group. The expression levels of osteocalcin, collagen I, Runt‑related transcription factor 2, Wnt family member 5A and calmodulin‑dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR‑26a‑5p inhibited bone formation while antimiR‑26a‑5p accelerated bone formation via the Wnt/Ca2+ signaling pathway. Therefore, antimiR‑26a‑5p‑modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue‑engineering bone graft for bone repair and reconstruction.
View Figures
View References

Related Articles

Journal Cover

September-2019
Volume 44 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yuan X, Han L, Lin H, Guo Z, Huang Y, Li S, Long T, Tang W, Tian W, Long J, Long J, et al: The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects. Int J Mol Med 44: 857-870, 2019
APA
Yuan, X., Han, L., Lin, H., Guo, Z., Huang, Y., Li, S. ... Long, J. (2019). The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects. International Journal of Molecular Medicine, 44, 857-870. https://doi.org/10.3892/ijmm.2019.4249
MLA
Yuan, X., Han, L., Lin, H., Guo, Z., Huang, Y., Li, S., Long, T., Tang, W., Tian, W., Long, J."The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects". International Journal of Molecular Medicine 44.3 (2019): 857-870.
Chicago
Yuan, X., Han, L., Lin, H., Guo, Z., Huang, Y., Li, S., Long, T., Tang, W., Tian, W., Long, J."The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects". International Journal of Molecular Medicine 44, no. 3 (2019): 857-870. https://doi.org/10.3892/ijmm.2019.4249