Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats

Chen,Sansan; Zhu,Jianbin; Wang,Mingzhu; Huang,Yanting; Qiu,Zhuolin; Li,Jingjing; Chen,Xinglu; Chen,Huiying; Xu,Mingyu; Liu,Jun; She,Miaoqin; Li,Hongwei; Yang,Xiaorong; Wang,Yi; Cai,Xiangsheng

doi:10.3892/ijmm.2019.4254

Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats

Authors:
- Sansan Chen
- Jianbin Zhu
- Mingzhu Wang
- Yanting Huang
- Zhuolin Qiu
- Jingjing Li
- Xinglu Chen
- Huiying Chen
- Mingyu Xu
- Jun Liu
- Miaoqin She
- Hongwei Li
- Xiaorong Yang
- Yi Wang
- Xiangsheng Cai
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Affiliations: Department of Urology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China, Technology Center, Guangdong Vitalife Bio‑Tech Co., Ltd., Foshan, Guangdong 528200, P.R. China, Reproductive Center of Obstetrics and Gynecology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Clinical Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China, Institute of Biotherapy, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Dermatology Hospital of Southern Medical University, Guangzhou, Guangdong 510091, P.R. China, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510660, P.R. China, Central Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China
Published online on: June 24, 2019 https://doi.org/10.3892/ijmm.2019.4254
Pages: 1006-1014
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to compare the effects of adipose‑derived mesenchymal stem cell (ADSC) and bone marrow mesenchymal stem cell (BMSC) transplantation into the corpora cavernosa of diabetic rats with erectile function. ADSCs and BMSCs were isolated and identified by flow cytometry. Rats with streptozocin‑induced diabetes were screened using apomorphine to obtain a rat model of diabetic erectile dysfunction, followed by transplantation of ADSCs and BMSCs into the corpora cavernosa. Two weeks later, the rats were again injected with apomorphine, the intracavernous pressure (ICP) and mean arterial pressure (MAP) of the penile tissue were measured, and the corpus cavernosum tissues were harvested. Angiogenic endothelial nitric oxide synthase (eNOS) expression was detected by western blotting and immunofluorescence analysis. The blood vessels in the corpus cavernosum were observed following hematoxylin and eosin (H&E) staining, and the expression of collagen was detected by Sirius Red staining. The cellular ultrastructure was examined by transmission electron microscopy. Intracavernous injection of ADSCs significantly increased ICP and ICP/MAP. Western blotting and immunofluorescence results revealed that ADSC treatment improved the expression of eNOS in the penile tissue of diabetic rats. The H&E staining results demonstrated that ADSC treatment promoted revascularization of the corpus cavernosum, and the results of Sirius Red staining revealed that ADSC treatment reduced penile collagen in diabetic rats. Transmission electron microscopy examination revealed that the ultrastructure of the tissues in the ADSC‑treated group was more complete compared with that in the untreated diabetic model group. In conclusion, ADSCs were found to be more effective compared with BMSCs in treating diabetes‑related erectile dysfunction.

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