Circular RNA profile in liver tissue of EpCAM knockout mice

Yang,Yanhong; Liu,Shaomin; Lei,Zili; Chen,Guibin; Huang,Li; Yang,Fei; Lei,Yuting; Liu,Yanyan; Yang,Lanxiang; Liu,Wanwan; Lai,Liangxue; Guo,Jiao

doi:10.3892/ijmm.2019.4270

Circular RNA profile in liver tissue of EpCAM knockout mice

Authors:
- Yanhong Yang
- Shaomin Liu
- Zili Lei
- Guibin Chen
- Li Huang
- Fei Yang
- Yuting Lei
- Yanyan Liu
- Lanxiang Yang
- Wanwan Liu
- Liangxue Lai
- Jiao Guo
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Affiliations: The First Affiliated Hospital (School of Clinical Medicine), Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China, Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine, Institute of Chinese Medicinal Sciences, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, P.R. China, Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, P.R. China
Published online on: July 8, 2019 https://doi.org/10.3892/ijmm.2019.4270
Pages: 1063-1077
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epithelial cell adhesion molecule (EpCAM) is highly expressed during liver development and carcinogenesis, However, its functions and underlying mechanisms remain unclear. Clustered regularly interspaced short palindromic repeats (CRISPRs)/CRISPR‑associated protein 9 (Cas9) technology was used in the current study to establish EpCAM‑/‑ mice. The expression of EpCAM in the livers of the mice at embryonic day (E)18.5 and post‑natal day (P)0 was detected by immunofluorescence staining. The expression of genes associated with the development and glycogen metabolism was also assessed by reverse transcription‑quantitative PCR. Additionally, the liver tissue of the EpCAM‑/‑ and wild‑type mice was used for non‑coding RNA sequencing. The results of RNA sequencing revealed 11 up‑regulated and 12 downregulated circular RNAs (circRNAs). Kyoto Encyclopedia of Genes and Genomes analysis for resource genes determined that the top altered pathways included cell junctions, cell cycle, immune signaling and metabolism. This analysis was also utilized to predict the target association of the circRNA‑microRNA‑mRNA network. The comprehensive liver tissue circRNA expression profiles produced in the present study may help to elucidate the functions and mechanisms of EpCAM during liver development.

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