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Article Open Access

miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1

Retraction in: /10.3892/ijmm.2025.5525
  • Authors:
    • Lihong Zhu
    • Shumei Yang
    • Jianfeng Wang
  • View Affiliations / Copyright

    Affiliations: The Second Clinical Medical College, Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi 712046, P.R. China, Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710038, P.R. China, Clinical Laboratory, People's Hospital of Tongchuan, Tongchuan, Shaanxi 727031, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1824-1832
    |
    Published online on: September 2, 2019
       https://doi.org/10.3892/ijmm.2019.4328
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Abstract

MicroRNA (miR)‑217 serves a pivotal role in the progression of colorectal cancer, renal cell carcinoma and glioma, however, the role of miR‑217 in cervical cancer (CC) remains unclear. In the present study, the mechanism of miR‑217 in cervical cancer was explored. The mRNA expression of miR‑217 and mitogen‑activated protein kinase 1 (MAPK1) were assessed using reverse transcription‑quantitative polymerase chain reaction analysis. Cell Counting‑Kit 8, wound‑healing and Transwell assays were performed to detect cell viability, migration and invasion, respectively. Apoptosis and cell cycle were determined by flow cytometry. TargetScan 7.2 and dual‑luciferase reporter assays were respectively used to determine miR‑217 target genes and their binding capacities. The protein expression levels of MAPK1, phosphorylated (p)‑extracellular signal‑regulated kinase 1/2 (ERK1/2)/ERK1/2, Bcl‑2, Bax and cleaved caspase‑3 were quantified by western blotting. It was found that miR‑217 was downregulated in patients with CC and in CC cells. The viability, migration and invasion of cells were suppressed by a miR‑217 mimic. It was also found that apoptosis was increased and cell cycle was inhibited by the miR‑217mimic, which was supported by changes in Bcl‑2, Bax and cleaved caspase‑3. MAPK1 was upregulated in patients with CC and was a target gene of miR‑217. MAPK1 reversed the inhibition of miR‑217 on cell viability, migration, invasion and apoptosis. The protein levels of MAPK1 and p‑ERK1/2, which were higher in the mimic MAPK1 group than those in the control or mimic groups, were ameliorated by PD98059. The results of the present study demonstrated that miR‑217 had an anti‑CC effect and may be effectively used in the treatment of CC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu L, Yang S and Wang J: miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525. Int J Mol Med 44: 1824-1832, 2019.
APA
Zhu, L., Yang, S., & Wang, J. (2019). miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525. International Journal of Molecular Medicine, 44, 1824-1832. https://doi.org/10.3892/ijmm.2019.4328
MLA
Zhu, L., Yang, S., Wang, J."miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525". International Journal of Molecular Medicine 44.5 (2019): 1824-1832.
Chicago
Zhu, L., Yang, S., Wang, J."miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525". International Journal of Molecular Medicine 44, no. 5 (2019): 1824-1832. https://doi.org/10.3892/ijmm.2019.4328
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu L, Yang S and Wang J: miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525. Int J Mol Med 44: 1824-1832, 2019.
APA
Zhu, L., Yang, S., & Wang, J. (2019). miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525. International Journal of Molecular Medicine, 44, 1824-1832. https://doi.org/10.3892/ijmm.2019.4328
MLA
Zhu, L., Yang, S., Wang, J."miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525". International Journal of Molecular Medicine 44.5 (2019): 1824-1832.
Chicago
Zhu, L., Yang, S., Wang, J."miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1 Retraction in /10.3892/ijmm.2025.5525". International Journal of Molecular Medicine 44, no. 5 (2019): 1824-1832. https://doi.org/10.3892/ijmm.2019.4328
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