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Article Open Access

Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats

  • Authors:
    • Shun‑Yuan Li
    • Zhi‑Xiao Li
    • Zhi‑Gang He
    • Qian Wang
    • Yu‑Juan Li
    • Qing Yang
    • Duo‑Zhi Wu
    • Hao‑Long Zeng
    • Hong‑Bing Xiang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Affiliated Quanzhou Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 470030, P.R. China, Department of Emergency Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 470030, P.R. China, College of Life Science, Wuhan University, Wuhan, Hubei 430076, P.R. China, Department of Anesthesiology, Hainan General Hospital, Haikou, Hainan 570311, P.R. China, Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 470030, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1877-1887
    |
    Published online on: September 17, 2019
       https://doi.org/10.3892/ijmm.2019.4341
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Abstract

There is now substantial evidence that myocardial ischemia‑reperfusion (IR) injury affects the spinal cord and brain, and that interactions may exist between these two systems. In the present study, the spinal cord proteomes were systematically analyzed after myocardial IR injury, in an attempt to identify the proteins involved in the processes. The myocardial IR injury rat model was first established by cross clamping the left anterior descending coronary artery for 30‑min ischemia, followed by reperfusion for 2 h, which resulted in a significant histopathological and functional myocardial injury. Then using the stable isotope dimethyl labeling quantitative proteomics strategy, a total of 2,362 shared proteins with a good distribution and correlation were successfully quantified. Among these proteins, 33 were identified which were upregulated and 57 were downregulated in the spinal cord after myocardial IR injury, which were involved in various biological processes, molecular function and cellular components. Based on these proteins, the spinal cord protein interaction network regulated by IR injury, including apoptosis, microtubule dynamics, stress‑activated signaling and cellular metabolism was established. These heart‑spinal cord interactions help explain the apparent randomness of cardiac events and provide new insights into future novel therapies to prevent myocardial I/R injury.
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Copy and paste a formatted citation
Spandidos Publications style
Li SY, Li ZX, He ZG, Wang Q, Li YJ, Yang Q, Wu DZ, Zeng HL and Xiang HB: Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats. Int J Mol Med 44: 1877-1887, 2019.
APA
Li, S., Li, Z., He, Z., Wang, Q., Li, Y., Yang, Q. ... Xiang, H. (2019). Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats. International Journal of Molecular Medicine, 44, 1877-1887. https://doi.org/10.3892/ijmm.2019.4341
MLA
Li, S., Li, Z., He, Z., Wang, Q., Li, Y., Yang, Q., Wu, D., Zeng, H., Xiang, H."Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats". International Journal of Molecular Medicine 44.5 (2019): 1877-1887.
Chicago
Li, S., Li, Z., He, Z., Wang, Q., Li, Y., Yang, Q., Wu, D., Zeng, H., Xiang, H."Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats". International Journal of Molecular Medicine 44, no. 5 (2019): 1877-1887. https://doi.org/10.3892/ijmm.2019.4341
Copy and paste a formatted citation
x
Spandidos Publications style
Li SY, Li ZX, He ZG, Wang Q, Li YJ, Yang Q, Wu DZ, Zeng HL and Xiang HB: Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats. Int J Mol Med 44: 1877-1887, 2019.
APA
Li, S., Li, Z., He, Z., Wang, Q., Li, Y., Yang, Q. ... Xiang, H. (2019). Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats. International Journal of Molecular Medicine, 44, 1877-1887. https://doi.org/10.3892/ijmm.2019.4341
MLA
Li, S., Li, Z., He, Z., Wang, Q., Li, Y., Yang, Q., Wu, D., Zeng, H., Xiang, H."Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats". International Journal of Molecular Medicine 44.5 (2019): 1877-1887.
Chicago
Li, S., Li, Z., He, Z., Wang, Q., Li, Y., Yang, Q., Wu, D., Zeng, H., Xiang, H."Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats". International Journal of Molecular Medicine 44, no. 5 (2019): 1877-1887. https://doi.org/10.3892/ijmm.2019.4341
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