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Article Open Access

Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy

  • Authors:
    • Yaoxu Chen
    • Huimin Beng
    • Hao Su
    • Fuping Han
    • Zhuo Fan
    • Nanying Lv
    • Aleksandar Jovanović
    • Wen Tan
  • View Affiliations / Copyright

    Affiliations: Department of Innovative Drugs and Medicine, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong 510641, P.R. China, Department of Basic and Clinical Sciences, University of Nicosia Medical School, CY‑1700 Nicosia, Cyprus
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1932-1942
    |
    Published online on: September 17, 2019
       https://doi.org/10.3892/ijmm.2019.4342
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Abstract

Isosteviol sodium (STVNa), which is a derivate of the natural sweet‑tasting glycoside stevioside, has recently been developed and it has been determined that this compound exhibits neuro‑ and cardio‑protective properties. In the current study, whether STVNa interferes with the development of cardiac hypertrophy, which is induced by isoprenaline (Iso), was investigated in an experimental rat model. Rats were treated with a vehicle (0.9% NaCl; control), isoprenaline (Iso; 5 mg/kg) or Iso (5 mg/kg) with STVNa (4 mg/kg; Iso + STVNa). Cardiomyocytes were isolated using enzymatic dissociation and were treated with 5 µM Iso for 24 h and co‑treated with 5 µM STVNa. Brain natriuretic peptide (BNP) mRNA expression was determined using PCR analysis. Cell surface area, intracellular reactive oxygen species (ROS), mitochondrial transmembrane potential (ΔΨm), cytoplasmic Ca2+ and Ca2+ and contractile function were examined using a laser scanning confocal microscope. The current study demonstrated that STVNa inhibited Iso‑induced cardiac hypertrophy by inhibiting cardiomyocyte size. STVNa significantly reduced cell surface area and decreased BNP mRNA expression in ventricular cardiomyocyte Iso‑induced hypertrophy. STVNa was also revealed to restore ΔΨm and reduce ROS generation and intracellular Ca2+ concentration when compared with the Iso‑treated group. Additionally, STVNa preserved Ca2+ transients in hypertrophic cardiomyocytes. In conclusion, the present study demonstrated that STVNa protects against Iso‑induced myocardial hypertrophy by reducing oxidative stress, restoring ΔΨm and maintaining Ca2+ homeostasis.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Beng H, Su H, Han F, Fan Z, Lv N, Jovanović A and Tan W: Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy. Int J Mol Med 44: 1932-1942, 2019.
APA
Chen, Y., Beng, H., Su, H., Han, F., Fan, Z., Lv, N. ... Tan, W. (2019). Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy. International Journal of Molecular Medicine, 44, 1932-1942. https://doi.org/10.3892/ijmm.2019.4342
MLA
Chen, Y., Beng, H., Su, H., Han, F., Fan, Z., Lv, N., Jovanović, A., Tan, W."Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy". International Journal of Molecular Medicine 44.5 (2019): 1932-1942.
Chicago
Chen, Y., Beng, H., Su, H., Han, F., Fan, Z., Lv, N., Jovanović, A., Tan, W."Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy". International Journal of Molecular Medicine 44, no. 5 (2019): 1932-1942. https://doi.org/10.3892/ijmm.2019.4342
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Beng H, Su H, Han F, Fan Z, Lv N, Jovanović A and Tan W: Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy. Int J Mol Med 44: 1932-1942, 2019.
APA
Chen, Y., Beng, H., Su, H., Han, F., Fan, Z., Lv, N. ... Tan, W. (2019). Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy. International Journal of Molecular Medicine, 44, 1932-1942. https://doi.org/10.3892/ijmm.2019.4342
MLA
Chen, Y., Beng, H., Su, H., Han, F., Fan, Z., Lv, N., Jovanović, A., Tan, W."Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy". International Journal of Molecular Medicine 44.5 (2019): 1932-1942.
Chicago
Chen, Y., Beng, H., Su, H., Han, F., Fan, Z., Lv, N., Jovanović, A., Tan, W."Isosteviol prevents the development of isoprenaline‑induced myocardial hypertrophy". International Journal of Molecular Medicine 44, no. 5 (2019): 1932-1942. https://doi.org/10.3892/ijmm.2019.4342
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