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Fatty liver and alteration of the gut microbiome induced by diallyl disulfide

  • Authors:
    • Yanhong Yang
    • Fei Yang
    • Miaoling Huang
    • Huijuan Wu
    • Changyuan Yang
    • Xinyue Zhang
    • Lanxiang Yang
    • Guibin Chen
    • Shuqi Li
    • Qianyu Wang
    • Shaomin Liu
    • Yanyan Liu
    • Yuting Lei
    • Zili Lei
    • Jiao Guo
  • View Affiliations / Copyright

    Affiliations: School of Clinical Medicine, The First Affiliated Hospital, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China, Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine, Institute of Chinese Medicinal Sciences, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1908-1920
    |
    Published online on: September 24, 2019
       https://doi.org/10.3892/ijmm.2019.4350
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Abstract

Diallyl disulfide (DADS) is one of the primary components of garlic and it exhibits a broad range of biological activities. In the present study, the effects of DADS on lipid metabolism and its potential role in the modulation of the gut microbiome were determined. Hematoxylin and eosin and oil‑red O staining were used to assess the liver and intestinal tissues of mice treated with DADS. The expression of lipid metabolism‑associated genes was measured using reverse transcription‑quantitative PCR (RT‑qPCR). The effects of DADS on the gut microbiome were measured using 16S recombinant (r)DNA gene analysis. The results revealed that the serum non‑esterified free fatty acids, high density lipoprotein‑cholesterol, low density lipoprotein‑cholesterol, serum total cholesterol, liver triglyceride and total cholesterol levels of the mice fed with a low‑dose of DADS was significantly higher when compared with the control. Hematoxylin and eosin and oil‑red O staining demonstrated that DADS induced fatty liver in mice. The results of the RT‑qPCR revealed that the expression levels of a number of lipid metabolism‑associated genes were altered in the livers of mice treated with DADS. The 16S rDNA gene analysis demonstrated that the mice fed on a normal diet treated with a low‑dose of DADS had decreased levels of bacteria from the Bacteroidetes phyla and increased levels of bacteria from the Firmicutes phyla. The Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed the top 20 pathways enriched in the low‑dose DADS group of mice fed with a normal diet. In the present study, low‑dose DADS induced fatty liver and altered the gut microbiota, similar to the phenotype induced by a high fat diet, by regulating the expression of lipid metabolism associated genes.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Yang F, Huang M, Wu H, Yang C, Zhang X, Yang L, Chen G, Li S, Wang Q, Wang Q, et al: Fatty liver and alteration of the gut microbiome induced by diallyl disulfide. Int J Mol Med 44: 1908-1920, 2019.
APA
Yang, Y., Yang, F., Huang, M., Wu, H., Yang, C., Zhang, X. ... Guo, J. (2019). Fatty liver and alteration of the gut microbiome induced by diallyl disulfide. International Journal of Molecular Medicine, 44, 1908-1920. https://doi.org/10.3892/ijmm.2019.4350
MLA
Yang, Y., Yang, F., Huang, M., Wu, H., Yang, C., Zhang, X., Yang, L., Chen, G., Li, S., Wang, Q., Liu, S., Liu, Y., Lei, Y., Lei, Z., Guo, J."Fatty liver and alteration of the gut microbiome induced by diallyl disulfide". International Journal of Molecular Medicine 44.5 (2019): 1908-1920.
Chicago
Yang, Y., Yang, F., Huang, M., Wu, H., Yang, C., Zhang, X., Yang, L., Chen, G., Li, S., Wang, Q., Liu, S., Liu, Y., Lei, Y., Lei, Z., Guo, J."Fatty liver and alteration of the gut microbiome induced by diallyl disulfide". International Journal of Molecular Medicine 44, no. 5 (2019): 1908-1920. https://doi.org/10.3892/ijmm.2019.4350
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Yang F, Huang M, Wu H, Yang C, Zhang X, Yang L, Chen G, Li S, Wang Q, Wang Q, et al: Fatty liver and alteration of the gut microbiome induced by diallyl disulfide. Int J Mol Med 44: 1908-1920, 2019.
APA
Yang, Y., Yang, F., Huang, M., Wu, H., Yang, C., Zhang, X. ... Guo, J. (2019). Fatty liver and alteration of the gut microbiome induced by diallyl disulfide. International Journal of Molecular Medicine, 44, 1908-1920. https://doi.org/10.3892/ijmm.2019.4350
MLA
Yang, Y., Yang, F., Huang, M., Wu, H., Yang, C., Zhang, X., Yang, L., Chen, G., Li, S., Wang, Q., Liu, S., Liu, Y., Lei, Y., Lei, Z., Guo, J."Fatty liver and alteration of the gut microbiome induced by diallyl disulfide". International Journal of Molecular Medicine 44.5 (2019): 1908-1920.
Chicago
Yang, Y., Yang, F., Huang, M., Wu, H., Yang, C., Zhang, X., Yang, L., Chen, G., Li, S., Wang, Q., Liu, S., Liu, Y., Lei, Y., Lei, Z., Guo, J."Fatty liver and alteration of the gut microbiome induced by diallyl disulfide". International Journal of Molecular Medicine 44, no. 5 (2019): 1908-1920. https://doi.org/10.3892/ijmm.2019.4350
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