Open Access

Biological effects of melatonin on human adipose‑derived mesenchymal stem cells

  • Authors:
    • June Seok Heo
    • Sangshin Pyo
    • Ja‑Yun Lim
    • Dae Wui Yoon
    • Bo Yong Kim
    • Jin‑Hee Kim
    • Gi Jin Kim
    • Seung Gwan Lee
    • Jinkwan Kim
  • View Affiliations

  • Published online on: September 27, 2019     https://doi.org/10.3892/ijmm.2019.4356
  • Pages: 2234-2244
  • Copyright: © Heo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Mesenchymal stem cells (MSCs) are capable of differentiating into other cell types and exhibit immunomodulatory effects. MSCs are affected by several intrinsic and extrinsic signaling modulators, including growth factors, cytokines, extracellular matrix and hormones. Melatonin, produced by the pineal gland, is a hormone that regulates sleep cycles. Recent studies have shown that melatonin improves the therapeutic effects of stem cells. The present study aimed to investigate whether melatonin enhances the biological activities of human adipose‑derived MSCs. The results demonstrated that treatment with melatonin promoted cell proliferation by inducing SRY‑box transcription factor 2 gene expression and preventing replicative senescence. In addition, melatonin exerted anti‑adipogenic effects on MSCs. PCR analysis revealed that the expression of the CCAAT enhancer binding protein a gene, a key transcription factor in adipogenesis, was decreased following melatonin treatment, resulting in reduced adipogenic differentiation in an in vitro assay. The present study also examined the effect of melatonin on the immunomodulatory response using a co‑culture system of human peripheral blood mononuclear cells and MSCs. Activated T cells were strongly inhibited following melatonin exposure compared with those in the control group. Finally, the favorable effects of melatonin on MSCs were confirmed using luzindole, a selective melatonin receptor antagonist. The proliferation‑promoting, anti‑inflammatory effects of melatonin suggested that melatonin‑treated MSCs may be used for effective cell therapy.
View Figures
View References

Related Articles

Journal Cover

December-2019
Volume 44 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Heo JS, Pyo S, Lim JY, Yoon DW, Kim BY, Kim JH, Kim GJ, Lee SG and Kim J: Biological effects of melatonin on human adipose‑derived mesenchymal stem cells. Int J Mol Med 44: 2234-2244, 2019.
APA
Heo, J.S., Pyo, S., Lim, J., Yoon, D.W., Kim, B.Y., Kim, J. ... Kim, J. (2019). Biological effects of melatonin on human adipose‑derived mesenchymal stem cells. International Journal of Molecular Medicine, 44, 2234-2244. https://doi.org/10.3892/ijmm.2019.4356
MLA
Heo, J. S., Pyo, S., Lim, J., Yoon, D. W., Kim, B. Y., Kim, J., Kim, G. J., Lee, S. G., Kim, J."Biological effects of melatonin on human adipose‑derived mesenchymal stem cells". International Journal of Molecular Medicine 44.6 (2019): 2234-2244.
Chicago
Heo, J. S., Pyo, S., Lim, J., Yoon, D. W., Kim, B. Y., Kim, J., Kim, G. J., Lee, S. G., Kim, J."Biological effects of melatonin on human adipose‑derived mesenchymal stem cells". International Journal of Molecular Medicine 44, no. 6 (2019): 2234-2244. https://doi.org/10.3892/ijmm.2019.4356