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Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer

  • Authors:
    • Qing Han
    • Bangxing Huang
    • Zaiju Huang
    • Jing Cai
    • Lanqing Gong
    • Yifan Zhang
    • Jiahong Jiang
    • Weihong Dong
    • Zehua Wang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2245-2255
    |
    Published online on: October 2, 2019
       https://doi.org/10.3892/ijmm.2019.4361
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Abstract

Ascitic multicellular aggregates (MCAs) promote peritoneal metastasis of ovarian cancer. The aim of the present study was to elucidate the role of cancer‑associated fibroblasts (CAFs) in MCA formation and metastasis in patients with high‑grade serous ovarian cancer (HGSOC). Immunohistochemistry was used to identify the cell phenotypes and the presence of CAFs in ascitic MCAs. The role of CAFs in tumor‑cell MCA formation was assessed by co‑culture in suspension. Primary ascitic tumor cells and omental CAFs were used to generate ex vivo MCAs in hanging drops, and the invasiveness of MCAs was evaluated by mesothelial clearance and adhesion assays in vitro and in vivo. MCAs containing CAFs and tumor cells were identified in the ascitic fluid. CAFs facilitated tumor cell aggregation and compaction to form MCAs, and enhanced the mesothelial clearance and adhesion abilities of tumor‑cell MCAs. These findings suggest that ascitic CAFs promote peritoneal metastasis by forming heterotypic aggregates with tumor cells, and that they may serve as potential targets for the treatment of HGSOC.
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Copy and paste a formatted citation
Spandidos Publications style
Han Q, Huang B, Huang Z, Cai J, Gong L, Zhang Y, Jiang J, Dong W and Wang Z: Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer. Int J Mol Med 44: 2245-2255, 2019.
APA
Han, Q., Huang, B., Huang, Z., Cai, J., Gong, L., Zhang, Y. ... Wang, Z. (2019). Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer. International Journal of Molecular Medicine, 44, 2245-2255. https://doi.org/10.3892/ijmm.2019.4361
MLA
Han, Q., Huang, B., Huang, Z., Cai, J., Gong, L., Zhang, Y., Jiang, J., Dong, W., Wang, Z."Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer". International Journal of Molecular Medicine 44.6 (2019): 2245-2255.
Chicago
Han, Q., Huang, B., Huang, Z., Cai, J., Gong, L., Zhang, Y., Jiang, J., Dong, W., Wang, Z."Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer". International Journal of Molecular Medicine 44, no. 6 (2019): 2245-2255. https://doi.org/10.3892/ijmm.2019.4361
Copy and paste a formatted citation
x
Spandidos Publications style
Han Q, Huang B, Huang Z, Cai J, Gong L, Zhang Y, Jiang J, Dong W and Wang Z: Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer. Int J Mol Med 44: 2245-2255, 2019.
APA
Han, Q., Huang, B., Huang, Z., Cai, J., Gong, L., Zhang, Y. ... Wang, Z. (2019). Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer. International Journal of Molecular Medicine, 44, 2245-2255. https://doi.org/10.3892/ijmm.2019.4361
MLA
Han, Q., Huang, B., Huang, Z., Cai, J., Gong, L., Zhang, Y., Jiang, J., Dong, W., Wang, Z."Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer". International Journal of Molecular Medicine 44.6 (2019): 2245-2255.
Chicago
Han, Q., Huang, B., Huang, Z., Cai, J., Gong, L., Zhang, Y., Jiang, J., Dong, W., Wang, Z."Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer". International Journal of Molecular Medicine 44, no. 6 (2019): 2245-2255. https://doi.org/10.3892/ijmm.2019.4361
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