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Article

Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species

  • Authors:
    • Blendi Ura
    • Lorenzo Monasta
    • Giorgio Arrigoni
    • Danilo Licastro
    • Giovanni Di Lorenzo
    • Federico Romano
    • Bartolomea Gaita
    • Federica Scrimin
    • Giuseppe Ricci
  • View Affiliations / Copyright

    Affiliations: Institute for Maternal and Child Health‑IRCCS ‘Burlo Garofolo’, I‑34137 Trieste, Italy, Department of Biomedical Sciences, University of Padova, I‑35121 Padova, Italy, Centro di Biomedicina Molecolare, Area Science Park, I‑34149 Trieste, Italy
  • Pages: 2329-2335
    |
    Published online on: October 21, 2019
       https://doi.org/10.3892/ijmm.2019.4377
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Abstract

Uterine leiomyomas are benign smooth muscle cell tumors originating from the myometrium. The present study focused on leiomyoma and myometrium phosphoproteome enrichment by using immobilized metal affinity chromatography (IMAC). The phosphoproteome was analyzed by two‑dimensional gel electrophoresis coupled with mass spectrometry. Western blotting was used for data validation. The results from IMAC identified 26 proteins significantly differentially phosphorylated in leiomyomas compared with normal myometrium. Three upregulated proteins (peroxiredoxin 2, protein disulfide isomerase family A member 3 and peroxiredoxin 4) were further validated by western blotting. Ingenuity pathway analysis revealed that four phosphoproteins were involved in the inhibition of oxidative stress and synthesis of reactive oxygen species. The present results demonstated for the first time an association between oxidative stress and phosphorylation in leiomyoma development.
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Copy and paste a formatted citation
Spandidos Publications style
Ura B, Monasta L, Arrigoni G, Licastro D, Di Lorenzo G, Romano F, Gaita B, Scrimin F and Ricci G: Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species. Int J Mol Med 44: 2329-2335, 2019.
APA
Ura, B., Monasta, L., Arrigoni, G., Licastro, D., Di Lorenzo, G., Romano, F. ... Ricci, G. (2019). Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species. International Journal of Molecular Medicine, 44, 2329-2335. https://doi.org/10.3892/ijmm.2019.4377
MLA
Ura, B., Monasta, L., Arrigoni, G., Licastro, D., Di Lorenzo, G., Romano, F., Gaita, B., Scrimin, F., Ricci, G."Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species". International Journal of Molecular Medicine 44.6 (2019): 2329-2335.
Chicago
Ura, B., Monasta, L., Arrigoni, G., Licastro, D., Di Lorenzo, G., Romano, F., Gaita, B., Scrimin, F., Ricci, G."Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species". International Journal of Molecular Medicine 44, no. 6 (2019): 2329-2335. https://doi.org/10.3892/ijmm.2019.4377
Copy and paste a formatted citation
x
Spandidos Publications style
Ura B, Monasta L, Arrigoni G, Licastro D, Di Lorenzo G, Romano F, Gaita B, Scrimin F and Ricci G: Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species. Int J Mol Med 44: 2329-2335, 2019.
APA
Ura, B., Monasta, L., Arrigoni, G., Licastro, D., Di Lorenzo, G., Romano, F. ... Ricci, G. (2019). Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species. International Journal of Molecular Medicine, 44, 2329-2335. https://doi.org/10.3892/ijmm.2019.4377
MLA
Ura, B., Monasta, L., Arrigoni, G., Licastro, D., Di Lorenzo, G., Romano, F., Gaita, B., Scrimin, F., Ricci, G."Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species". International Journal of Molecular Medicine 44.6 (2019): 2329-2335.
Chicago
Ura, B., Monasta, L., Arrigoni, G., Licastro, D., Di Lorenzo, G., Romano, F., Gaita, B., Scrimin, F., Ricci, G."Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species". International Journal of Molecular Medicine 44, no. 6 (2019): 2329-2335. https://doi.org/10.3892/ijmm.2019.4377
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