Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
December-2019 Volume 44 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2019 Volume 44 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection

  • Authors:
    • Ting Zhang
    • Yuguo Yuan
    • Rui Lu
    • Sheng Xu
    • Minya Zhou
    • Tingting Yuan
    • Yaoyao Lu
    • Kunning Yan
    • Yong Cheng
  • View Affiliations / Copyright

    Affiliations: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China, Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2057-2064
    |
    Published online on: October 24, 2019
       https://doi.org/10.3892/ijmm.2019.4382
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

There is growing interest in the application of lactoferrin (LF) as a drug or food additive for animals and humans. The objective of this study was to produce transgenic cloned goats that would serve as living bioreactors, expressing high levels of recombinant human LF (rhLF) in their milk. We designed a pCL25 expression vector containing goat β‑casein/CMV chimeric promoter in order to facilitate rhLF expression. This pCL25‑rhLF‑Neo vector was microinjected into goat fetal fibroblasts. G418 selection and PCR analysis were used to identify transgenic donor cells suitable for somatic cell nuclear transfer (SCNT). After SCNT and embryo transplantation, goats harboring the hLF gene were produced, as confirmed via PCR and southern blotting. The average rhLF concentration in milk from this transgenic goat was 3.89 mg/ml as determined via ELISA. We also used an optimized buffer in order to effectively elute high‑purity (95.8%) rhLF from a cation‑exchange column, with the recovered rhLF exhibiting high biological activity. Findings from this study demonstrated that it is possible to generate a transgenic goat harboring the hLF transgene driven by the goat β‑casein/CMV chimeric promoter. It represents an initial step towards the production of rhLF, potentially allowing for industrialized purification in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

García-Montoya IA, Cendón TS, Arévalo-Gallegos S and Rascón-Cruz Q: Lactoferrin a multiple bioactive protein: An overview. Biochim Biophys Acta. 1820:226–236. 2012. View Article : Google Scholar

2 

Wu J, Hu Y, Du C, Piao J, Yang L and Yang X: The effect of recombinant human lactoferrin from the milk of transgenic cows on Salmonella enterica serovar typhimurium infection in mice. Food Funct. 7:308–314. 2016. View Article : Google Scholar

3 

Fliedl L, Grillari J and Grillari-Voglauer R: human cell lines for the production of recombinant proteins: On the horizon. New Biotechnol. 32:673–679. 2015. View Article : Google Scholar

4 

Fu Z, Baker D, Cheng A, Leighton J, Appelbaum E and Aon J: Characterization of a saccharomyces cerevisiae fermentation process for production of a therapeutic recombinant protein using a multivariate bayesian approach. Biotechnol Prog. 32:799–812. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Gupta SK and Shukla P: Advanced technologies for improved expression of recombinant proteins in bacteria: Perspectives and applications. Crit Rev Biotechnol. 36:1089–1098. 2016. View Article : Google Scholar

6 

Kim H, Yoo SJ and Kang HA: Yeast synthetic biology for the production of recombinant therapeutic proteins. FEMS Yeast Res. 15:1–16. 2015.

7 

Song S, Ge X and Cheng Y, Lu R, Zhang T, Yu B, Ji X, Qi Z, Rong Y, Yuan Y and Cheng Y: high-level expression of a novel recombinant human plasminogen activator (rhPA) in the milk of transgenic rabbits and its thrombolytic bioactivity in vitro. Mol Biol Rep. 43:775–783. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Lavine G: FDA approves first biological product derived from transgenic animal. Am J Health Syst Pharm. 66:5182009. View Article : Google Scholar : PubMed/NCBI

9 

Davis B and Bernstein JA: Conestat alfa for the treatment of angioedema attacks. Ther Clin Risk Manag. 7:265–273. 2011.PubMed/NCBI

10 

The farmyard drug store: Nature. 443:16–17. 2006. View Article : Google Scholar

11 

Bösze Z, Baranyi M and Whitelaw CB: Producing recombinant human milk proteins in the milk of livestock species. Adv Exp Med Biol. 606:357–393. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Pantano T, Rival-Gervier S, Prince S, Menck-Le Bourhis C, Maeder C, Viglietta C, Houdebine LM and Jolivet G: In vitro and in vivo effects of a multimerized alphas 1-casein enhancer on whey acidic protein gene promoter activity. Mol Reprod Dev. 65:262–268. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Huma N, Ghaffar F, Rafiq S, Pasha I, Sameen A, Hayat I and Hussain I: Characterization of milk proteins from different animal species through Gel electrophoresis. Pak J Zool. 50:1983–1986. 2018. View Article : Google Scholar

14 

Dayal R, Hurlimann J, Suard YM and Kraehenbuhl JP: Chemical and immunochemical characterization of caseins and the major whey proteins of rabbit milk. Biochem J. 201:71–79. 1982. View Article : Google Scholar : PubMed/NCBI

15 

Kung MH, Lee YJ, Hsu JT, Huang MC and JU YT: A functional study of proximal goat β-casein promoter and intron 1 in immortalized goat mammary epithelial cells. J Dairy Sci. 98:3859–3875. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Giraldo P and Montoliu L: Size matters: Use of YACs, BACs and PACs in transgenic animals. Transgenic Res. 10:83–103. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Fujiwara Y, Miwa M, Takahashi R, Kodaira K, Hirabayashi M, Suzuki T and Ueda M: high-level expressing YAC vector for transgenic animal bioreactors. Mol Reprod Dev. 52:414–420. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Sharp JA, Cane Kn, Mailer SL, Oosthuizen WH, Arnould JP and Nicholas KR: Species-specific cell-matrix interactions are essential for differentiation of alveoli like structures and milk gene expression in primary mammary cells of the Cape fur seal (Arctocephalus pusillus pusillus). Matrix Biol. 25:430–442. 2006. View Article : Google Scholar : PubMed/NCBI

19 

McKnight RA, Shamay A, Sankaran L, Wall RJ and Hennighausen L: Matrix-attachment regions can impart position- independent regulation of a tissue-specific gene in transgenic mice. Proc Natl Acad Sci USA. 89:6943–6947. 1992. View Article : Google Scholar

20 

Zarrin AA, Malkin L, Fong I, Luk KD, Ghose A and Berinstein NL: Comparison of CMV, RSV, SV40 viral and Vlambda1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochim Biophys Acta. 1446:135–139. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Law BA and Reiter B: The isolation and bacteriostatic properties of lactoferrin from bovine milk whey. J Dairy Res. 44:595–599. 1977. View Article : Google Scholar : PubMed/NCBI

22 

Yoshida S, Wei Z, Shinmura Y and Fukunaga N: Separation of lactoferrin-a and -b from bovine colostrum. J Dairy Sci. 83:2211–2215. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Nuijens JH, van Berkel PH and Schanbacher FL: Structure and biological actions of lactoferrin. J Mammary Gland Biol neoplasia. 1:285–295. 1996. View Article : Google Scholar : PubMed/NCBI

24 

Aguila A, Herrera AG, Toledo W, Rodríguez AA, Garcia HM, Hernández J, Cádiz A, Sierra G and Brock JH: Isolation and structure-functional characterization of human colostral lacto-ferrin. Biotecnol Appl. 17:177–182. 2000.

25 

Cheng Y, An LY, Yuan YG, Wang Y, Du FL, Yu BL, Zhang ZH, Huang YZ and Yang TJ: hybrid expression cassettes consisting of a milk protein promoter and a cytomegalovirus enhancer significantly increase mammary-specific expression of human lactoferrin in transgenic mice. Mol Reprod Dev. 79:573–585. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Yuan YG, An L, Yu B, Song S, Zhou F, Zhang L, Gu Y, Yu M and Cheng Y: Expression of recombinant human alpha-lactalbumin in the milk of transgenic goats using a hybrid pomoter/enhancer. J Anal Methods Chem. 2014:2810312014. View Article : Google Scholar : PubMed/NCBI

27 

Gomez HF, Ochoa TJ, Herrera-Insua I, Carlin LG and Cleary TG: Lactoferrin protects rabbits from Shigella flexneri-induced inflammatory enteritis. Infect Immun. 70:7050–7053. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Ochoa TJ, Brown EL, Guion CE, Chen JZ, McMahon RJ and Cleary TG: Effect of lactoferrin on enteroaggregative E. coli (EAEC). Biochem Cell Biol. 84:369–376. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Ochoa TJ, Noguera-Obenza M, Ebel F, Guzman CA, Gomez HF and Cleary TG: Lactoferrin impairs type III secretory system function in enteropathogenic Escherichia coli. Infect Immun. 71:5149–5155. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Farnaud S and Evans RW: Lactoferrin-a multifunctional protein with antimicrobial properties. Mol Immunol. 40:395–405. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Mosquito S, Ochoa TJ, Cok J and Cleary TG: Effect of bovine lactoferrin in Salmonella ser. Typhimurium infection in mice. Biometals. 23:515–521. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Nuijens JH, van Berkel PH, Geerts ME, Hartevelt PP, de Boer HA, van Veen HA and Pieper FR: Characterization of recombinant human lactoferrin secreted in milk of transgenic mice. J Biol Chem. 272:8802–8807. 1997. View Article : Google Scholar : PubMed/NCBI

33 

Li L, Shen W, Min L, Dong H, Sun Y and Pan Q: human lactoferrin transgenic rabbits produced efficiently using dimethylsulfoxide- sperm-mediated gene transfer. Reprod Fertil Dev. 18:689–695. 2006. View Article : Google Scholar

34 

van Berkel PH, Welling MM, Geerts M, van Veen HA, Ravensbergen B, Salaheddine M, Pauwels EK, Pieper F, Nuijens JH and Nibbering PH: Large scale production of recombinant human lactoferrin in the milk of transgenic cows. Nat Biotechnol. 20:484–487. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Pastuszka R, Barłowska J and Litwińczuk Z: Allergenicity of milk of different animal species in relation to human milk. Postepy Hig Med Dosw (online). 70:1451–1459. 2016. View Article : Google Scholar

36 

Høst A, Husby S, Gjesing B, Larsen JN and Løwenstein H: Prospective estimation of IgG, IgG subclass and IgE antibodies to dietary proteins in infants with cow milk allergy. Levels of antibodies to whole milk protein, BLG and ovalbumin in relation to repeated milk challenge and clinical course of cow milk allergy. Allergy. 47:218–229. 1992. View Article : Google Scholar

37 

Pisanu S, Marogna G, Pagnozzi D, Piccinini M, Leo G, Tanca A, Roggio AM, Roggio T, Uzzau S and Addis MF: Characterization of size and composition of milk fat globules from Sarda and Saanen dairy goats. Small Ruminant Res. 109:141–151. 2013. View Article : Google Scholar

38 

Juvarajah T, Wan-Ibrahim WI, Ashrafzadeh A, Othman S, Hashim OH, Fung SY and Abdul-Rahman PS: Human milk fat globule membrane contains hundreds of abundantly expressed and nutritionally beneficial proteins that are generally lacking in caprine milk. Breastfeed Med. 13:631–637. 2018. View Article : Google Scholar : PubMed/NCBI

39 

Schramm FR: The Dolly case, the Polly drug, and the morality of human cloning. Cad Saude Publica. 15(Suppl 1): 51–64. 1999. View Article : Google Scholar : PubMed/NCBI

40 

An LY, Yuan YG, Yu BL, Yang TJ and Cheng Y: Generation of human lactoferrin transgenic cloned goats using donor cells with dual markers and a modified selection procedure. Theriogenology. 78:1303–1311. 2012. View Article : Google Scholar : PubMed/NCBI

41 

Ohkoshi K, Takahashi S, Koyama S, Akagi S, Adachi N, Furusawa T, Fujimoto J, Takeda K, Kubo M, Izaike Y and Tokunaga T: In vitro oocyte culture and somatic cell nuclear transfer used to produce a live-born cloned goat. Cloning Stem Cells. 5:109–115. 2003. View Article : Google Scholar : PubMed/NCBI

42 

Wang M, Sun Z, Yu T, Ding F, Li L, Wang X, Fu M, Wang H, Huang J, Li N and Dai Y: Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows. Sci Rep. 7:107332017. View Article : Google Scholar : PubMed/NCBI

43 

Gordon JW: Studies of foreign genes transmitted through the germ lines of transgenic mice. J Exp Zool. 228:313–324. 1983. View Article : Google Scholar : PubMed/NCBI

44 

Hu J, Peng X, Schell TD, Budgeon LR, Cladel NM and Christensen ND: An hLA-A2.1-transgenic rabbit model to study immunity to papillomavirus infection. J Immunol. 177:8037–8045. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Yanagimachi R: Cloning: Experience from the mouse and other animals. Mol Cell Endocrinol. 187:241–248. 2002. View Article : Google Scholar : PubMed/NCBI

46 

Oback B and Wells D: Donor cells for nuclear cloning: Many are called, but few are chosen. Cloning Stem Cells. 4:147–168. 2002. View Article : Google Scholar : PubMed/NCBI

47 

Westhusin ME, Long CR, Shin T, Hill JR, Looney CR, Pryor JH and Piedrahita JA: Cloning to reproduce desired genotypes. Theriogenology. 55:35–49. 2001. View Article : Google Scholar : PubMed/NCBI

48 

Robertson G, Garrick D, Wu W, Kearns M, Martin D and Whitelaw E: Position-dependent variegation of globin transgene expression in mice. Proc Natl Acad Sci USA. 92:5371–5375. 1995. View Article : Google Scholar : PubMed/NCBI

49 

Dobie KW, Lee M, Fantes JA, Graham E, Clark AJ, Springbett A, Lathe R and McClenaghan M: Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus. Proc Natl Acad Sci USA. 93:6659–6664. 1996. View Article : Google Scholar : PubMed/NCBI

50 

Zhang J, Li L, Cai Y, Xu X, Chen J, Wu Y, Yu H, Yu G, Liu S, Zhang A, et al: Expression of active recombinant human lacto-ferrin in the milk of transgenic goats. Protein Expr Purif. 57:127–135. 2008. View Article : Google Scholar

51 

Tomita M, Wakabayashi H, Yamauchi K, Teraguchi S and Hayasawa H: Bovine lactoferrin and lactoferricin derived from milk: Production and applications. Biochem Cell Biol. 80:109–112. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang T, Yuan Y, Lu R, Xu S, Zhou M, Yuan T, Lu Y, Yan K and Cheng Y: The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection. Int J Mol Med 44: 2057-2064, 2019.
APA
Zhang, T., Yuan, Y., Lu, R., Xu, S., Zhou, M., Yuan, T. ... Cheng, Y. (2019). The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection. International Journal of Molecular Medicine, 44, 2057-2064. https://doi.org/10.3892/ijmm.2019.4382
MLA
Zhang, T., Yuan, Y., Lu, R., Xu, S., Zhou, M., Yuan, T., Lu, Y., Yan, K., Cheng, Y."The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection". International Journal of Molecular Medicine 44.6 (2019): 2057-2064.
Chicago
Zhang, T., Yuan, Y., Lu, R., Xu, S., Zhou, M., Yuan, T., Lu, Y., Yan, K., Cheng, Y."The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection". International Journal of Molecular Medicine 44, no. 6 (2019): 2057-2064. https://doi.org/10.3892/ijmm.2019.4382
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang T, Yuan Y, Lu R, Xu S, Zhou M, Yuan T, Lu Y, Yan K and Cheng Y: The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection. Int J Mol Med 44: 2057-2064, 2019.
APA
Zhang, T., Yuan, Y., Lu, R., Xu, S., Zhou, M., Yuan, T. ... Cheng, Y. (2019). The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection. International Journal of Molecular Medicine, 44, 2057-2064. https://doi.org/10.3892/ijmm.2019.4382
MLA
Zhang, T., Yuan, Y., Lu, R., Xu, S., Zhou, M., Yuan, T., Lu, Y., Yan, K., Cheng, Y."The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection". International Journal of Molecular Medicine 44.6 (2019): 2057-2064.
Chicago
Zhang, T., Yuan, Y., Lu, R., Xu, S., Zhou, M., Yuan, T., Lu, Y., Yan, K., Cheng, Y."The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection". International Journal of Molecular Medicine 44, no. 6 (2019): 2057-2064. https://doi.org/10.3892/ijmm.2019.4382
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team